{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T22:46:29Z","timestamp":1791758789512,"version":"4.3.4"},"reference-count":57,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T00:00:00Z","timestamp":1750118400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T00:00:00Z","timestamp":1750118400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Shanghai Pudong New Area Science and Technology Development Fund Special Project for People's Livelihood Scientific Research","award":["PKJ2024-Y10"],"award-info":[{"award-number":["PKJ2024-Y10"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSystems"],"published-print":{"date-parts":[[2025,6,17]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Cervical intraepithelial neoplasia (CIN) is a common gynecological condition often associated with persistent human papillomavirus (HPV) infections. Although the loop electrosurgical excision procedure (LEEP) is effective in removing lesions, some patients remain HPV positive post-treatment. In this prospective study, we enrolled reproductive-age women diagnosed with HPV-related CIN and employed a multi-omics analysis of cervicovaginal secretion and cervical tissue microbiomes, alongside non-targeted and targeted metabolomic assessments. We aim to explore the role of the cervicovaginal and intratissue microbiota and associated metabolites on HPV clearance following LEEP. We observed significant shifts in bacterial diversity and composition in both cervicovaginal secretion and cervical tissue samples. Notably, distinct bacterial species, such as\n                      <jats:italic toggle=\"yes\">Lactobacillus<\/jats:italic>\n                      and certain anaerobes (e.g.,\n                      <jats:italic toggle=\"yes\">Prevotella bivia<\/jats:italic>\n                      ), were correlated with HPV clearance post-LEEP. Metabolomic profiling revealed that the HPV-cleared group exhibited elevated acetic acid levels and significant alterations in glycerophospholipid metabolism, suggesting a potential role in promoting HPV clearance. Correlation analyses demonstrated significant associations between altered bacteria and metabolites with HPV status, with models incorporating both achieving high predictive accuracy. Overall, our findings highlight the importance of the cervicovaginal and intratissue microbiomes and metabolites in facilitating HPV clearance, suggesting potential therapeutic targets for patients with CIN.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      The clearance of human papillomavirus (HPV) after local excisional treatment for cervical intraepithelial neoplasia is crucial for patient health. This study reveals significant alterations in the cervicovaginal secretion and cervical tissue microbiomes, alongside metabolomic changes, which are associated with HPV clearance. Through a comprehensive multi-omics approach, we identified specific bacterial species and metabolic changes that correlate with successful HPV clearance post-loop electrosurgical excision procedure. Notably, the presence of beneficial\n                      <jats:italic toggle=\"yes\">Lactobacillus<\/jats:italic>\n                      species and elevated levels of acetic acid linked to glycerophospholipid metabolism emerged as potential biomarkers for HPV status, suggesting that these factors play a pivotal role in improving treatment outcomes. These findings highlight the potential for microbiome-targeted therapies to enhance HPV clearance and provide insights into the microbial and metabolic mechanisms involved in cervical health.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/msystems.00511-25","type":"journal-article","created":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T09:00:18Z","timestamp":1747731618000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Microbiome and metabolomic changes associated with HPV clearance in women undergoing local excisional treatment for cervical intraepithelial neoplasia"],"prefix":"10.1128","volume":"10","author":[{"given":"Xiaowen","family":"Pu","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03rc6as71","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Gynecology, Shanghai First Maternity and Infant Hospital, School of Medicine, 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