{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T13:16:28Z","timestamp":1785417388725,"version":"3.56.0"},"reference-count":50,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2025,8,19]],"date-time":"2025-08-19T00:00:00Z","timestamp":1755561600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,8,19]],"date-time":"2025-08-19T00:00:00Z","timestamp":1755561600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"the Key Research and Development Project of Jiangsu Provincial Scinence and Technology Department","award":["BE2023704"],"award-info":[{"award-number":["BE2023704"]}]},{"name":"the Project of MOE Key Laboratory of Geriatric Diseases and Immunology of Soochow University","award":["KJS2403"],"award-info":[{"award-number":["KJS2403"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSystems"],"published-print":{"date-parts":[[2025,8,19]]},"abstract":"ABSTRACT<\/jats:title>\n \n \n \n The emergence of metallo-beta-lactamase gene variants, such as\n bla<\/jats:italic>\n IMP-26<\/jats:sub>\n , has posed a significant challenge to bacterial infection treatment, drawing considerable attention in public health. This study aims to investigate the resistance mechanism of\n bla<\/jats:italic>\n IMP-26<\/jats:sub>\n -carrying clinical\n Enterobacter xiangfangensis<\/jats:italic>\n in China.\n bla<\/jats:italic>\n IMP-26<\/jats:sub>\n -harboring carbapenem-resistant\n Enterobacteriaceae<\/jats:italic>\n (CRE) isolates were collected from a multicenter study across China. The\n bla<\/jats:italic>\n IMP-26<\/jats:sub>\n was identified by polymerase chain reaction (PCR). Multi-locus sequence typing (MLST) and phylogenetic analysis were conducted to investigate the genetic evolutionary characterization of\n Enterobacteriaceae<\/jats:italic>\n carrying\n bla<\/jats:italic>\n IMP-26<\/jats:sub>\n . The genomic contexts of these strains were explored by whole genome sequencing, while conjugation and plasmid stability assays were conducted to assess the transferability and maintenance of the resistance plasmids. Furthermore, the antibiotic susceptibility of IMP-26 to \u03b2-lactams was evaluated through antimicrobial susceptibility testing, enzyme kinetic analysis, and molecular docking. Five\n bla<\/jats:italic>\n IMP-26<\/jats:sub>\n -carrying strains were collected, and all were multidrug-resistant\n Enterobacter xiangfangensis. bla<\/jats:italic>\n IMP-26<\/jats:sub>\n was located either on IncHI2\/2A or the novel Inc\n pKPC-CAV1321<\/jats:sub>\n plasmid, both exhibiting high and stable self-transfer frequency. In addition,\n bla<\/jats:italic>\n IMP-26<\/jats:sub>\n was identified within a novel class 1 integron In437, or the classical integron In837. Antimicrobial susceptibility testing and enzyme kinetic assay further showed that IMP-26 could mediate high levels of resistance to common carbapenem and cephalosporin antibiotics. This study characterized the genomic and clinical characteristics of\n bla<\/jats:italic>\n IMP-26<\/jats:sub>\n -carrying clinical\n Enterobacter xiangfangensis<\/jats:italic>\n in China. The high carbapenem resistance and transmission capacity of\n bla<\/jats:italic>\n IMP-26<\/jats:sub>\n highlight the need for enhanced surveillance and preventive measures to contain the spread of\n bla<\/jats:italic>\n IMP-26<\/jats:sub>\n .\n <\/jats:p>\n \n IMPORTANCE<\/jats:title>\n \n Our research has led to the documentation of a novel IncpKPC-CAV1321 plasmid and the discovery of a novel integron In437, both carrying the\n bla<\/jats:italic>\n IMP-26<\/jats:sub>\n gene. A comprehensive analysis of the carbapenem resistance levels and enzymatic kinetics exhibited by IMP-26 revealed that IMP-26 could mediate high levels of resistance to common carbapenem and cephalosporin antibiotics. Our findings underscore the critical need for enhanced surveillance and preventive measures to curtail the dissemination of\n bla<\/jats:italic>\n IMP-26<\/jats:sub>\n .\n <\/jats:p>\n <\/jats:sec>\n <\/jats:sec>","DOI":"10.1128\/msystems.00578-25","type":"journal-article","created":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T13:02:41Z","timestamp":1753966961000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["High level of carbapenem resistance and transmission ability of\n bla<\/i>\n IMP-26<\/sub>\n in multidrug-resistant\n Enterobacter xiangfangensis<\/i>\n isolates from China"],"prefix":"10.1128","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0363-3001","authenticated-orcid":false,"given":"Qizhao","family":"Gao","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02xjrkt08","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University","place":["Suzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yicheng","family":"Wen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02xjrkt08","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University","place":["Suzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaxuan","family":"Zhai","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02xjrkt08","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University","place":["Suzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feinan","family":"Qian","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02xjrkt08","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University","place":["Suzhou, China"]},{"name":"Department of Clinical Laboratory, Zhenjiang First People's Hospital","place":["Zhenjiang, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangxiang","family":"Tian","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02xjrkt08","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University","place":["Suzhou, China"]},{"name":"Department of Blood Transfusion, Anhui Chest Hospital","place":["Hefei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhichen","family":"Zhu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02xjrkt08","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University","place":["Suzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Zhu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02xjrkt08","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University","place":["Suzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Jia","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02h8a1848","id-type":"ROR","asserted-by":"publisher"}],"name":"Center of Medical Laboratory, General Hospital of Ningxia Medical University","place":["Yinchuan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Tao","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02h8a1848","id-type":"ROR","asserted-by":"publisher"}],"name":"Center of Medical Laboratory, General Hospital of Ningxia Medical University","place":["Yinchuan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Yu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04qr3zq92","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Laboratory Medicine and Sichuan Provincial Key Laboratory for Human Disease Gene Study, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofang","family":"Xie","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02xjrkt08","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University","place":["Suzhou, China"]},{"id":[{"id":"https:\/\/ror.org\/02xjrkt08","id-type":"ROR","asserted-by":"publisher"}],"name":"MOE Key Laboratory of Geriatric Diseases and Immunology, The Second Affiliated Hospital of Soochow University","place":["Suzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Chen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01y64my43","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pharmacy Practice, School of Pharmacy and Pharmaceutical Sciences, University at Buffalo","place":["Buffalo, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2973-1523","authenticated-orcid":true,"given":"Hong","family":"Du","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02xjrkt08","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University","place":["Suzhou, China"]},{"id":[{"id":"https:\/\/ror.org\/02xjrkt08","id-type":"ROR","asserted-by":"publisher"}],"name":"MOE Key Laboratory of Geriatric Diseases and Immunology, The Second Affiliated Hospital of Soochow University","place":["Suzhou, China"]},{"name":"Key Laboratory of Alkene-Carbon Fibres-Based Technology and Application for Detection of Major Infectious Diseases","place":["Suzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00296-11"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2020.12.009"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2013.01.005"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkad326"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01905-09"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt269"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dky303"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.3343\/alm.2012.32.4.270"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01177-16"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.01610"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2017.03.002"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.hlife.2024.10.003"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.hlife.2023.11.004"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2406.171648"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph192214812"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkae008"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.03529-23"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2022.2148562"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.000760"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab902"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/0471250953.bi1003s00"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy096"},{"key":"e_1_3_5_24_2","unstructured":"Lewis JS Weinstein MP Bobenchik AM Campeau S Cullen SK Galas MF Gold H Humphries RM Kirn TJ Limbago B Mathers AJ Mazzulli T Richter SS Satlin M Schuetz AN Sharp S Simner PJ Tamma PD Weinstein MP. 2025. Performance standards for antimicrobial susceptibility testing"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2018.1475261"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07487-w"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/Spectrum.01305-21"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep08365"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt282"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-3167-5_2"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1189"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw1004"},{"key":"e_1_3_5_33_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks261"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/mlf2.12046"},{"key":"e_1_3_5_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp105"},{"key":"e_1_3_5_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj014"},{"key":"e_1_3_5_37_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-12-402"},{"key":"e_1_3_5_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku316"},{"key":"e_1_3_5_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2015.053"},{"key":"e_1_3_5_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.hlife.2024.06.005"},{"key":"e_1_3_5_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2019.03.005"},{"key":"e_1_3_5_42_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.586504"},{"key":"e_1_3_5_43_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9050947"},{"key":"e_1_3_5_44_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13099-021-00458-w"},{"key":"e_1_3_5_45_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkg169"},{"key":"e_1_3_5_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01801-14"},{"key":"e_1_3_5_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.hlife.2024.04.001"},{"key":"e_1_3_5_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scib.2023.10.038"},{"key":"e_1_3_5_49_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jctc.3c00300"},{"key":"e_1_3_5_50_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciz826"},{"key":"e_1_3_5_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02011-21"}],"container-title":["mSystems"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/msystems.00578-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/msystems.00578-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,19]],"date-time":"2025-08-19T13:01:06Z","timestamp":1755608466000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/msystems.00578-25"}},"subtitle":[],"editor":[{"given":"Youjun","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,8,19]]},"references-count":50,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,8,19]]}},"alternative-id":["10.1128\/msystems.00578-25"],"URL":"https:\/\/doi.org\/10.1128\/msystems.00578-25","relation":{},"ISSN":["2379-5077"],"issn-type":[{"value":"2379-5077","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,19]]},"assertion":[{"value":"2025-04-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00578-25"}}