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Mass medication with antibiotics is a common practice to control BRD in dairy cattle. However, the emergence of multidrug-resistant BRD pathogens highlights the importance of developing alternatives to antibiotics for BRD mitigation. Using a targeted approach, we recently identified 6<jats:italic>Lactobacillus<\/jats:italic>strains originating from the bovine respiratory microbiota as candidates to be used as bacterial therapeutics (BTs) for the mitigation of the BRD pathogen<jats:named-content content-type=\"genus-species\">Mannheimia haemolytica<\/jats:named-content>. Here, we demonstrated that intranasal inoculation of the BT strains reduced nasal colonization by<jats:italic>M. haemolytica<\/jats:italic>in dairy calves experimentally challenged with this pathogen. This study, for the first time, shows the potential use of intranasal BTs as an alternative to mitigate BRD pathogens in cattle.<\/jats:p>","DOI":"10.1128\/msystems.00629-19","type":"journal-article","created":{"date-parts":[[2020,2,29]],"date-time":"2020-02-29T10:09:46Z","timestamp":1582970986000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Intranasal Bacterial Therapeutics Reduce Colonization by the Respiratory Pathogen Mannheimia haemolytica in Dairy Calves"],"prefix":"10.1128","volume":"5","author":[{"given":"Samat","family":"Amat","sequence":"first","affiliation":[{"name":"Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, Alberta, Canada"},{"name":"Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trevor W.","family":"Alexander","sequence":"additional","affiliation":[{"name":"Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5306-3732","authenticated-orcid":false,"given":"Devin B.","family":"Holman","sequence":"additional","affiliation":[{"name":"Lacombe Research and Development Centre, Agriculture and Agri-Food Canada, Lacombe, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy","family":"Schwinghamer","sequence":"additional","affiliation":[{"name":"Agriculture and Agri-Food Canada, Lethbridge Research and Development Centre, Lethbridge, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edouard","family":"Timsit","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Calgary, Calgary, Alberta, Canada"},{"name":"Simpson Ranch Chair in Beef Cattle Health and Wellness, University of Calgary, Calgary, Alberta, Canada"},{"name":"CEVA Sant\u00e9 Animale, Libourne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2018-14774"},{"key":"e_1_3_2_3_2","volume-title":"Dairy heifer raiser, 2011","author":"USDA","year":"2012","unstructured":"USDA. 2012. 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