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The metabolomic capabilities of this phylum remain poorly characterized, with few experimentally confirmed metabolites described. To address these gaps, we combined untargeted metabolomic profiling with comparative genomic analyses to explore the functional potential of newly isolated\n                      <jats:italic toggle=\"yes\">Acidobacteriaceae<\/jats:italic>\n                      strains. Genome mining across the\n                      <jats:italic toggle=\"yes\">Acidobacteriota<\/jats:italic>\n                      phylum revealed the presence and taxon-specific enrichment of genes associated with plant-related traits, including phytohormone biosynthesis. In parallel, metabolomic analyses of OSMAC-derived extracts uncovered pronounced condition-dependent metabolic variation. Tryptophan supplementation was associated with marked metabolomic reprogramming, including changes in indole-derived metabolites, such as indole-3-acetic acid. Subsequent analyses linked these metabolic shifts to the suppression of phytopathogenic fungi and enabled the identification of malassezindoles and pityriacitrins as active compounds, confirmed by structure elucidation using NMR spectroscopy. Overall, these findings shed light on the previously unexplored metabolic potential of the\n                      <jats:italic toggle=\"yes\">Acidobacteriota<\/jats:italic>\n                      phylum, emphasizing its ecological importance for phytosanitary applications.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      Despite their ubiquity and genomic diversity, the functional metabolism of members of the\n                      <jats:italic toggle=\"yes\">Acidobacteriota<\/jats:italic>\n                      has largely remained uncharacterized. This study links genomic predictions to experimentally verified metabolomic outputs of\n                      <jats:italic toggle=\"yes\">Acidobacteriaceae<\/jats:italic>\n                      , demonstrating tryptophan-responsive metabolic shifts translating to phytohormones and metabolites suppressing fungal growth. Our work underscores the emerging role of\n                      <jats:italic toggle=\"yes\">Acidobacteriota<\/jats:italic>\n                      as important contributors to soil ecosystem functioning and plant\u2013microbe interactions.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/msystems.00828-26","type":"journal-article","created":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T13:01:05Z","timestamp":1789390865000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Tryptophan-driven metabolomic shift in\n                    <i>Acidobacteriaceae<\/i>\n                    reveals phytohormones and antifungal metabolites"],"prefix":"10.1128","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7906-575X","authenticated-orcid":false,"given":"Celine M.","family":"Zumkeller","sequence":"first","affiliation":[{"name":"Institute for Insect Biotechnology with Focus on 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