{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T02:22:10Z","timestamp":1784600530647,"version":"3.55.0"},"reference-count":61,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2020,12,22]],"date-time":"2020-12-22T00:00:00Z","timestamp":1608595200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,12,22]],"date-time":"2020-12-22T00:00:00Z","timestamp":1608595200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81630057"],"award-info":[{"award-number":["81630057"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"MOST | National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2018YFA0507304"],"award-info":[{"award-number":["2018YFA0507304"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSystems"],"published-print":{"date-parts":[[2020,12,22]]},"abstract":"<jats:p>\n            Studies on the trophozoite of\n            <jats:named-content content-type=\"genus-species\">Entamoeba histolytica<\/jats:named-content>\n            suggested this organism could accumulate polyploid cells in its proliferative phase and differentiate its cell cycle from that of other eukaryotes. Therefore, a single-cell sequencing technique was used to study the switching of the RNA transcription profiles of single amoebic trophozoites.\n          <\/jats:p>","DOI":"10.1128\/msystems.01095-20","type":"journal-article","created":{"date-parts":[[2020,12,22]],"date-time":"2020-12-22T20:56:55Z","timestamp":1608670615000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Single-Cell RNA Sequencing Reveals that the Switching of the Transcriptional Profiles of Cysteine-Related Genes Alters the Virulence of Entamoeba histolytica"],"prefix":"10.1128","volume":"5","author":[{"given":"Meng","family":"Feng","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongfeng","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1807-9090","authenticated-orcid":false,"given":"Hiroshi","family":"Tachibana","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Tokai University School of Medicine, Isehara, Kanagawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8851-6903","authenticated-orcid":false,"given":"Xunjia","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Fudan University, Shanghai, China"},{"name":"Department of Infectious Diseases, Tokai University School of Medicine, Isehara, Kanagawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005616"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005110"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)12830-9"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/9781444354690"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2009.06.008"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2005.03.020"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep35852"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2019.00034"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-091313-103550"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-15923-3"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature13242"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004360050462"},{"key":"e_1_3_3_14_2","first-page":"159","article-title":"A monoclonal antibody against the 150-kDa surface antigen of Entamoeba histolytica inhibits adherence and cytotoxicity to mammalian cells","volume":"25","author":"Cheng XJ","year":"1997","unstructured":"Cheng XJ, Kaneda Y, Tachibana H. 1997. A monoclonal antibody against the 150-kDa surface antigen of Entamoeba histolytica inhibits adherence and cytotoxicity to mammalian cells. Med Sci Res 25:159\u2013161.","journal-title":"Med Sci Res"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1999.60.1.9988319"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.69.9.5892-5898.2001"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182007003459"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.4.4.722-732.2005"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0040008"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2009.12.007"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00025-06"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0092-8674(00)00114-8"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.18.012502.083431"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M102381200"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2017.07.003"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2017.07.002"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.48994"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2017.01.023"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2017.07.006"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.43803"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2018.04.017"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-01415-5"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.02.001"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2017-315631"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1717871115"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.28415"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-03243-7"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature24280"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2006.02.007"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2008.12.007"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003824"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2008.01236.x"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000266"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2011.06.003"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2005.12.012"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-012-0349-1"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01335-06"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2013.04.021"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/cmr.15.3.329-341.2002"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004419"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms16027"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2015.09.011"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03814.x"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1038\/357689a0"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.03029.x"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0001-706x(02)00237-1"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.2.801"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004360000323"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4236"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2012.016"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.04758-14"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr070378x"}],"container-title":["mSystems"],"original-title":[],"contributor":[{"given":"Mario Alberto","family":"Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"reviewer"}]}],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mSystems.01095-20","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/mSystems.01095-20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,14]],"date-time":"2022-03-14T19:55:56Z","timestamp":1647287756000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/mSystems.01095-20"}},"subtitle":[],"editor":[{"given":"Marta M.","family":"Gaglia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,12,22]]},"references-count":61,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2020,12,22]]}},"alternative-id":["10.1128\/mSystems.01095-20"],"URL":"https:\/\/doi.org\/10.1128\/msystems.01095-20","relation":{},"ISSN":["2379-5077"],"issn-type":[{"value":"2379-5077","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,12,22]]},"assertion":[{"value":"2020-10-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01095-20"}}