{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T15:42:59Z","timestamp":1789054979452,"version":"build-2803163510"},"reference-count":70,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T00:00:00Z","timestamp":1734393600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T00:00:00Z","timestamp":1734393600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"School Science and Technology Team Project of Inner Mongolia Medcal University","award":["YKD2022TD026"],"award-info":[{"award-number":["YKD2022TD026"]}]},{"name":"General Project of Inner Mongolia Medical University","award":["YKD2021MS011"],"award-info":[{"award-number":["YKD2021MS011"]}]},{"name":"Inner Mongolia \u201cPrairie Elite\u201d Project Youth Innovation and Entrepreneurship Talent Training Program","award":["ZY0130013"],"award-info":[{"award-number":["ZY0130013"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["mSystems"],"published-print":{"date-parts":[[2024,12,17]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Carbapenem-resistant hypervirulent\n              <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n              (CR-hvKP) strains present a significant global public health threat due to their high mortality rates. This study investigated the genomic characteristics of seven ST11-K1 CR-hvKP isolates harboring highly homologous KPC-2-encoding multidrug-resistance plasmids. The strains were isolated from a Chinese tertiary hospital between 2017 and 2020. Whole-genome sequencing and bioinformatic analysis revealed various antibiotic resistance genes (ARGs) and virulence determinants. The\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>KPC-2<\/jats:sub>\n              -bearing plasmids that contain multiple antibiotic-resistance genes were also identified in these strains. ISfinder and Orifinder were applied to identify insertion sequences (IS) and conjugation-related factors among these\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>KPC-2<\/jats:sub>\n              -bearing plasmids. The\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>KPC-2<\/jats:sub>\n              was highly consistent in seven\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>KPC-2<\/jats:sub>\n              -bearing plasmids (IS\n              <jats:italic>Kpn6-bla<\/jats:italic>\n              <jats:sub>KPC-2<\/jats:sub>\n              -IS\n              <jats:italic>Kpn27<\/jats:italic>\n              -IS\n              <jats:italic>Yps3<\/jats:italic>\n              -IS\n              <jats:italic>26<\/jats:italic>\n              ). In addition, we found a region composed of IS\n              <jats:italic>IR<\/jats:italic>\n              , Tn\n              <jats:italic>5393<\/jats:italic>\n              , and IS\n              <jats:italic>26<\/jats:italic>\n              . It was located upstream of the\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>CTX-M-15<\/jats:sub>\n              gene and presented in six\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>KPC-2<\/jats:sub>\n              -bearing plasmids, with pCR-hvKP221-KPC-P3 as an exception. Conjugation experiments demonstrated the horizontal transfer of resistance plasmids pCR-hvKP128-KPC-P1 and pCR-hvKP132-KPC-P1 across species. Notably, pLVPK-like virulence plasmids carrying virulence gene clusters pCR-hvKP173-Vir-P1, and pCR-hvKP221-Vir-P1 were also detected. A fusional plasmid pCR-hvKP221-Vir-P2, which carries virulence gene clusters and ARGs, was also identified. Five CR-hvKP strains displayed enhanced biofilm formation and high virulence\n              <jats:italic>in vivo<\/jats:italic>\n              infection models. Phylogenetic and single nucleotide polymorphism (SNP) analyses indicated a close genetic relationship among the isolates, suggesting a subclade. These findings highlight the complex genetic profiles and potential transmission mechanisms of CR-hvKP strains.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              We reported seven CR-hvKP strains all carried a highly homologous\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>KPC-2<\/jats:sub>\n              integrated IncF\u2161-resistant plasmid, and two strains harbored virulence plasmids. Conjugation experiments confirmed the transferability of these plasmids, indicating a potential for resistance spread. Phylogenetic analysis clarified the relationship among the CR-hvKP isolates. This study provides insights into the phenotypic and genomic characteristics of seven ST11-K1 CR-hvKP strains. The high prevalence and potential for local outbreaks emphasize the need for effective control measures.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/msystems.01101-24","type":"journal-article","created":{"date-parts":[[2024,11,18]],"date-time":"2024-11-18T14:00:11Z","timestamp":1731938411000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Phenotypic and genomic characterization of ST11-K1 CR-hvKP with highly homologous\n            <i>bla<\/i>\n            <sub>KPC-2<\/sub>\n            -bearing plasmids in China"],"prefix":"10.1128","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-5239-3074","authenticated-orcid":false,"given":"Yu-Ling","family":"Han","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, The Affiliated Hospital of Inner Mongolia Medical University","place":["Hohhot, China"]},{"name":"Department of Parasitology, The Basic 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