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Here, we have used the genetically and biochemically tractable infection model system\n                    <jats:italic>Dictyostelium discoideum<\/jats:italic>\n                    \/\n                    <jats:italic>Mycobacterium marinum<\/jats:italic>\n                    to apply a genome-wide transposon-sequencing experimental strategy to reveal comprehensively which mutations confer a fitness advantage or disadvantage during infection and compare these to a similar experiment performed using the murine microglial BV2 cells as host for\n                    <jats:italic>M. marinum<\/jats:italic>\n                    to identify conservation of virulence pathways between hosts.\n                  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