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TB infections present on a spectrum from active to latent disease. In the human host,\n                      <jats:italic toggle=\"yes\">Mtb<\/jats:italic>\n                      faces hostile environments, such as nutrient deprivation, hypoxia, and low pH. Under these conditions,\n                      <jats:italic toggle=\"yes\">Mtb<\/jats:italic>\n                      can enter a dormant, but viable, state characterized by a lack of cell replication and increased resistance to antibiotics. Dormant\n                      <jats:italic toggle=\"yes\">Mtb<\/jats:italic>\n                      poses a major challenge to curing infections and eradicating TB globally. We subjected\n                      <jats:italic toggle=\"yes\">Mtb<\/jats:italic>\n                      mc\n                      <jats:sup>2<\/jats:sup>\n                      6020 (\u0394\n                      <jats:italic toggle=\"yes\">lysA<\/jats:italic>\n                      and \u0394\n                      <jats:italic toggle=\"yes\">panCD<\/jats:italic>\n                      ), a double auxotrophic strain, to carbon starvation (CS), a culture condition that induces growth stasis and mimics environmental conditions associated with dormancy\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      . We provide a detailed analysis of the proteome in CS compared to replicating samples. We observed extensive proteomic reprogramming, with 36% of identified proteins significantly altered in CS. Many enzymes involved in oxidative phosphorylation and lipid metabolism were retained or more abundant in CS. The cell wall biosynthetic machinery was present in CS, although numerous changes in the abundance of peptidoglycan, arabinogalactan, and mycolic acid biosynthetic enzymes likely result in pronounced remodeling of the cell wall. Many clinically approved anti-TB drugs target cell wall biosynthesis, and we found that these enzymes were largely retained in CS. Lastly, we compared our results to those of other dormancy models and propose that CS produces a physiologically distinct state of stasis compared to hypoxia in\n                      <jats:italic toggle=\"yes\">Mtb<\/jats:italic>\n                      .\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Tuberculosis is a devastating human disease that kills over 1.2 million people a year. This disease is caused by the bacterial pathogen\n                        <jats:italic toggle=\"yes\">Mycobacterium tuberculosis<\/jats:italic>\n                        (\n                        <jats:italic toggle=\"yes\">Mtb<\/jats:italic>\n                        ).\n                        <jats:italic toggle=\"yes\">Mtb<\/jats:italic>\n                        excels at surviving in the human host by entering a non-replicating, dormant state. The current work investigated the proteomic changes that\n                        <jats:italic toggle=\"yes\">Mtb<\/jats:italic>\n                        undergoes in response to carbon starvation, a culture condition that models dormancy. The authors found broad effects of carbon starvation on the proteome, with the relative abundance of 37% of proteins significantly altered. Protein changes related to cell wall biosynthesis, metabolism, and drug susceptibility are discussed. Proteins associated with a carbon starvation phenotype are identified, and results are compared to other dormancy models, including hypoxia.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/msystems.01530-24","type":"journal-article","created":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T09:00:16Z","timestamp":1744707616000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Proteomic characterization of\n                    <i>Mycobacterium tuberculosis<\/i>\n                    subjected to carbon starvation"],"prefix":"10.1128","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5842-0591","authenticated-orcid":false,"given":"Kaylyn L.","family":"Devlin","sequence":"first","affiliation":[{"name":"Department of Chemical Physiology and Biochemistry, Oregon Health and Science University","place":["Portland, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4752-0380","authenticated-orcid":false,"given":"Damon T.","family":"Leach","sequence":"additional","affiliation":[{"name":"Biological Sciences Division, Pacific Northwest National Laboratory","place":["Richland, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1721-9688","authenticated-orcid":false,"given":"Kelly G.","family":"Stratton","sequence":"additional","affiliation":[{"name":"Biological Sciences Division, Pacific Northwest National Laboratory","place":["Richland, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2214-0114","authenticated-orcid":true,"given":"Gyanu","family":"Lamichhane","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Johns Hopkins University","place":["Baltimore, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9159-0031","authenticated-orcid":true,"given":"Vivian S.","family":"Lin","sequence":"additional","affiliation":[{"name":"Biological Sciences Division, Pacific Northwest National Laboratory","place":["Richland, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2303-8779","authenticated-orcid":true,"given":"Kimberly E.","family":"Beatty","sequence":"additional","affiliation":[{"name":"Department of Chemical Physiology and Biochemistry, Oregon Health and Science University","place":["Portland, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","unstructured":"World Health Organization. 2024. 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