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The genetic environment, transcriptome, mobile, and resistome were characterized. The MicroScan system, disc diffusion, and broth microdilution were used to determine the resistance profile of the isolate. A multiplex PCR assay was also used to screen for carbapenemases and\n              <jats:italic>mcr<\/jats:italic>\n              -1 to -5 resistance genes. Efflux-pump inhibitors were used to evaluate efflux activity. The resistome, mobilome, epigenome, and transcriptome were characterized. There was phenotypic resistance to 22 of the 25 antibiotics tested, intermediate resistance to levofloxacin and nalidixic acid, and susceptibility to tigecycline, which corresponded to the 27 resistance genes found in the genome, most of which occurred in multiple copies through replicative transposition. A plasmid-borne (pR-B2.MM_C3)\n              <jats:italic>mcr-<\/jats:italic>\n              1 and chromosomal\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>PER-7<\/jats:sub>\n              <jats:italic>, bla<\/jats:italic>\n              <jats:sub>OXA-69<\/jats:sub>\n              <jats:italic>, bla<\/jats:italic>\n              <jats:sub>OXA-23<\/jats:sub>\n              (three copies),\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>ADC-25<\/jats:sub>\n              ,\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>TEM-1B<\/jats:sub>\n              , and\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>NDM-1<\/jats:sub>\n              were found within composite transposons, ISs, and\/or class 1 and 2 integrons on genomic islands. Types I and II methylases and restriction endonucleases were in close synteny to these resistance genes within the genomic islands; chromosomal genomic islands aligned with known plasmids. There was a closer evolutionary relationship between the strain and global strains but not local or regional strains; the resistomes also differed. Significantly expressed\/repressed genes (6.2%) included resistance genes, hypothetical proteins, mobile elements, methyltransferases, transcription factors, and membrane and efflux proteins. The genomic evolution observed in this strain explains its adaptability and pandrug resistance and shows its genomic plasticity on exposure to antibiotics.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>A pandrug-resistant pathogen that was susceptible to only a single antibiotic, tigecycline, was isolated from a middle-aged patient in an ICU. This pathogen contained two plasmids and had a chromosome that contained portions that were integrated externally from plasmids. These genomic islands were rich with resistance genes, mobile genetic elements, and restriction-modification systems that protected the pathogen and facilitated gene regulation. The strain contained 35 resistance genes and 12 virulence genes. The strain was of closer evolutionary distance to several international strains suggesting that it was imported into South Africa. However, its resistome was unique, suggesting an independent evolution on exposure to antibiotic therapy mediated by epigenomic factors and MGE transposition events. The varied mechanisms available to this strain to overcome antibiotic resistance and spread to other areas and\/or transfer its resistance determinants are worrying. This is ultimately a risk to public health, evincing the need for antibiotic stewardship.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/msystems.01683-24","type":"journal-article","created":{"date-parts":[[2025,2,24]],"date-time":"2025-02-24T14:01:10Z","timestamp":1740405670000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["Plasmid-borne\n            <i>mcr-1<\/i>\n            and replicative transposition of episomal and chromosomal\n            <i>bla<\/i>\n            <sub>NDM-1<\/sub>\n            ,\n            <i>bla<\/i>\n            <sub>OXA-69<\/sub>\n            , and\n            <i>bla<\/i>\n            <sub>OXA-23<\/sub>\n            carbapenemases in a clinical\n            <i>Acinetobacter baumannii<\/i>\n            isolate"],"prefix":"10.1128","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5238-454X","authenticated-orcid":true,"given":"Masego","family":"Mmatli","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, School of Medicine, University of Pretoria","place":["Pretoria, South Africa"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nontombi Marylucy","family":"Mbelle","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, School of Medicine, University of Pretoria","place":["Pretoria, South Africa"]},{"name":"Department of Medical Microbiology, Tshwane Academic Division, National Health Laboratory Service","place":["Pretoria, South Africa"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9508-984X","authenticated-orcid":false,"given":"John","family":"Osei Sekyere","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, School of Medicine, University of Pretoria","place":["Pretoria, South Africa"]},{"name":"Institute of Biomarker Research and Clinical Development, Medical Diagnostic Laboratories, Genesis Biotechnology Group","place":["Hamilton Township, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2010.04.196"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra070741"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjid.2019.09.004"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/500202"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2020.101451"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01388-10"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1590\/0037-8682-0237-2019"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.7196\/AJTCCM.2021.v27i1.122"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00897-20"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/1756-0500-5-215"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1086\/533545"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/7380740"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.01280"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2019.11.021"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01083-19"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00133-19"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkz413"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2409.171386"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2019.0243"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2022.0112"},{"key":"e_1_3_5_22_2","volume-title":"Performance standards for antimicrobial susceptibility testing","author":"CLSI & Clinical and Laboratory Standards Institute (CLSI)","year":"2023","unstructured":"CLSI & Clinical and Laboratory Standards Institute (CLSI). 2023. 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