{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T22:35:54Z","timestamp":1792794954914,"version":"4.5.0"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T00:00:00Z","timestamp":1738627200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T00:00:00Z","timestamp":1738627200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"MOST | National Key Research and Development Program of China","award":["No.2022YFC2303600"],"award-info":[{"award-number":["No.2022YFC2303600"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,2,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              The clustered regularly interspaced short palindromic repeats (CRISPR)\u2012CRISPR-associated protein (Cas) and restriction\u2012modi\ufb01cation (R\u2013M) systems are important immune systems in bacteria. Information about the distributions of these two systems in\n              <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n              from different hosts and their mutual effect on antibiotic resistance and virulence is still limited. In this study, the whole genomes of 520 strains of\n              <jats:italic>K. pneumoniae<\/jats:italic>\n              from GenBank, including 325 from humans and 195 from animals, were collected for CRISPR\u2012Cas systems and type I R-M systems, virulence genes, antibiotic resistance genes, and multilocus sequence typing detection. The results showed that host origin had no obvious influence on the distributions of the two systems (CRISPR\u2012Cas systems in 29.8% and 24.1%, type I R-M systems in 9.8% and 11.8% of human-origin and animal-origin strains, respectively) in\n              <jats:italic>K. pneumoniae<\/jats:italic>\n              . Identical spacer sequences from different hosts demonstrated there was a risk of human\u2013animal transmission. All virulence genes (yersiniabactin, colibactin, aerobactin, salmochelin,\n              <jats:italic>rmpADC<\/jats:italic>\n              , and\n              <jats:italic>rmpA2<\/jats:italic>\n              ) detection rates were higher when only the CRISPR\u2012Cas systems were present but were all reduced when coexisting with type I R-M systems. However, a lower prevalence of most antibiotic-resistance genes was found when the CRISPR\u2012Cas systems were alone, and when type I R-M systems were coexisting, some of the antibiotic resistance gene incidence rates were even lower (quinolones, macrolides, tetracyclines and carbapenems), and some of them were higher instead (aminoglycosides, clindamycins, rifampicin-associated, sulfonamides, methotrexates, beta-lactamases and ultrabroad-spectrum beta-lactamases). The synergistic and opposed effects of the two systems on virulence and antibiotic-resistance genes need further study.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                <jats:italic>K. pneumoniae<\/jats:italic>\n                is an important opportunistic pathogen responsible for both human and animal infections, and the emergence of hypervirulent and multidrug-resistant\n                <jats:italic>K. pneumoniae<\/jats:italic>\n                has made it difficult to control this pathogen worldwide. Here, we find that CRISPR\u2012Cas and restriction\u2013modification systems, which function as adaptive and innate immune systems in bacteria, have synergistic and opposed effects on virulence and antibiotic resistance genes in\n                <jats:italic>K. pneumoniae<\/jats:italic>\n                . Moreover, this study provides insights into the distributions of the two systems in\n                <jats:italic>K. pneumoniae<\/jats:italic>\n                from different hosts, and there is no significant difference in the prevalence of the two systems among\n                <jats:italic>K. pneumoniae<\/jats:italic>\n                spp. In addition, this study also characterizes the CRISPR arrays of\n                <jats:italic>K. pneumoniae<\/jats:italic>\n                from different hosts, suggesting that the strains sharing the same spacer sequences have the potential to spread between humans and animals.\n              <\/jats:p>\n            <\/jats:sec>\n          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