{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T02:53:28Z","timestamp":1788836008898,"version":"build-2803163510"},"reference-count":61,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:00:00Z","timestamp":1783382400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:00:00Z","timestamp":1783382400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100014103","name":"Shandong Province Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2024CXPT061"],"award-info":[{"award-number":["2024CXPT061"]}],"id":[{"id":"10.13039\/100014103","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Outstanding Youth Found of Jiangsu Provincial Natural Science Foundation","award":["BK20240090"],"award-info":[{"award-number":["BK20240090"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,7,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Vibrio parahaemolyticus<\/jats:italic>\n                      is an important zoonotic pathogen that poses a persistent threat to aquaculture systems and food safety. The rapid emergence of multidrug resistance, driven by increasing antibiotic selection pressure, highlights the urgent need for alternative antimicrobial strategies, among which phage therapy has emerged as a promising candidate. Here, we reported the isolation of a bacteriophage, designated \u03a6170, targeting multidrug-resistant\n                      <jats:italic toggle=\"yes\">Vibrio parahaemolyticus<\/jats:italic>\n                      from sewage collected at a shrimp farm. \u03a6170 exhibited a latent period of 10 min, an eclipse period of approximately 60 min, and an average burst size of 426 PFU per cell. The phage also showed broad tolerance to pH and temperature. \u03a6170 displayed rapid and potent lytic activity, effectively suppressing host bacterial growth within 6 h post-infection. To further assess its in\n                      <jats:italic toggle=\"yes\">vivo<\/jats:italic>\n                      efficacy, we evaluated the prophylactic and therapeutic potential of phage administration by immersion in zebrafish challenged with\n                      <jats:italic toggle=\"yes\">Vibrio parahaemolyticus<\/jats:italic>\n                      . Prophylactic treatment with \u03a6170 significantly increased zebrafish survival within 72 h. Moreover, combined treatment with \u03a6170 and doxycycline exerted a synergistic effect, effectively reducing bacterial loads, alleviating liver and intestine tissue damage, and suppressing excessive inflammatory responses. Collectively, these findings indicated that phage \u03a6170 represented a promising prophylactic agent against\n                      <jats:italic toggle=\"yes\">Vibrio parahaemolyticus<\/jats:italic>\n                      infection and that combined phage therapy with antibiotics provided an effective strategy for treating drug-resistant\n                      <jats:italic toggle=\"yes\">Vibrio parahaemolyticus<\/jats:italic>\n                      infections.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Phage therapy is a promising strategy for combating bacterial infections, serving as a complementary therapy to antibiotics and a synergistic approach in combination treatments with antimicrobial agents. To address infections by multidrug-resistant (MDR)\n                        <jats:italic toggle=\"yes\">Vibrio parahaemolyticus<\/jats:italic>\n                        , we isolated and characterized bacteriophage \u03a6170, which is capable of lysing MDR\n                        <jats:italic toggle=\"yes\">Vibrio parahaemolyticus<\/jats:italic>\n                        . We evaluated its prophylactic and therapeutic potential in a zebrafish infection model. We found that phage \u03a6170 exhibited strong lytic activity both\n                        <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                        and\n                        <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                        , effectively preventing\n                        <jats:italic toggle=\"yes\">Vibrio parahaemolyticus<\/jats:italic>\n                        infection and displaying synergistic antibacterial effects when combined with antibiotics. Phage-antibiotic combination therapy further enhanced therapeutic efficacy in infected zebrafish. Our results provide experimental support for phage-antibiotic combination strategies against MDR bacterial infections and highlight the potential of phage therapy for the prevention and treatment of\n                        <jats:italic toggle=\"yes\">Vibrio parahaemolyticus<\/jats:italic>\n                        infections.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.00089-26","type":"journal-article","created":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T13:01:42Z","timestamp":1779800502000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Phage \u03a6170 biocontrol of multidrug-resistant\n                    <i>Vibrio parahaemolyticus<\/i>\n                    in a zebrafish model"],"prefix":"10.1128","volume":"14","author":[{"given":"Wanqiu","family":"Lin","sequence":"first","affiliation":[{"name":"MOE Joint 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