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While N-acetylglucosamine (GlcNAc), an ubiquitous amino sugar, serves as a vital nutrient, its catabolic pathway in\n                      <jats:italic toggle=\"yes\">A. fumigatus<\/jats:italic>\n                      remains unexplored. Here, we identified core components of this pathway, including GlcNAc-6-phosphate deacetylase (DacA), glucosamine-6-phosphate deaminase (NagA), and the transcription factor RonA. The expressions of\n                      <jats:italic toggle=\"yes\">dacA<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">nagA<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">ronA<\/jats:italic>\n                      were strongly induced when GlcNAc was the sole carbon source. Both \u0394\n                      <jats:italic toggle=\"yes\">dacA<\/jats:italic>\n                      and \u0394\n                      <jats:italic toggle=\"yes\">nagA<\/jats:italic>\n                      mutants exhibited abolished growth under GlcNAc condition, whereas the \u0394\n                      <jats:italic toggle=\"yes\">ronA<\/jats:italic>\n                      mutant exhibited pleiotropic defects, including severe growth defects, impaired polarity, delayed development, reduced cell wall integrity, and decreased virulence in a\n                      <jats:italic toggle=\"yes\">Galleria mellonella<\/jats:italic>\n                      infection model. The deletion of\n                      <jats:italic toggle=\"yes\">ronA<\/jats:italic>\n                      resulted in enhanced immune clearance and exacerbated inflammatory responses. Conidial cell wall analysis revealed that \u0394\n                      <jats:italic toggle=\"yes\">ronA<\/jats:italic>\n                      conidia displayed aberrant cell wall architecture, primarily characterized by increased surface protein exposure and significantly reduced melanin. Collectively, our findings highlight RonA\u2019s critical role in GlcNAc catabolism, conidial cell wall integrity, and the pathogenesis of\n                      <jats:italic toggle=\"yes\">A. fumigatus<\/jats:italic>\n                      , providing novel insights into antifungal drug development.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        <jats:italic toggle=\"yes\">Aspergillus fumigatus<\/jats:italic>\n                        is a major human fungal pathogen known for its ability to cause a wide range of diseases, primarily due to its exceptional adaptability to diverse environments. This study identifies DacA and NagA as key enzymes in GlcNAc catabolism, while the transcription factor RonA is essential for growth, sporulation, and cell wall stress response on GlcNAc. Beyond regulating GlcNAc catabolism, RonA was found to play a pivotal role in modifying the conidial cell wall structure, influencing host-pathogen interactions, including immune modulation and pathogenicity. These findings highlight RonA as a potential therapeutic target for treating\n                        <jats:italic toggle=\"yes\">A. fumigatus<\/jats:italic>\n                        infections.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.00122-25","type":"journal-article","created":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T10:48:31Z","timestamp":1759920511000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Transcription factor RonA-driven GlcNAc catabolism is essential for growth, cell wall integrity, and pathogenicity in\n                    <i>Aspergillus fumigatus<\/i>"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-1471-4813","authenticated-orcid":false,"given":"Xiufang","family":"Gong","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/054x1kd82","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Biological Sciences and Technology, Guangxi Academy of Sciences","place":["Nanning, China"]},{"id":[{"id":"https:\/\/ror.org\/00yd0p282","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinwei","family":"Ge","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/054x1kd82","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Biological Sciences and Technology, Guangxi Academy of Sciences","place":["Nanning, China"]},{"id":[{"id":"https:\/\/ror.org\/02c9qn167","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Life Science and Technology, Guangxi University","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qijian","family":"Qin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/054x1kd82","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Biological Sciences and Technology, Guangxi Academy of Sciences","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Wang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/054x1kd82","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Biological Sciences and Technology, Guangxi Academy of Sciences","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5243-341X","authenticated-orcid":true,"given":"Linqi","family":"Wang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00yd0p282","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1514-6374","authenticated-orcid":true,"given":"Cheng","family":"Jin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00yd0p282","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8055-2072","authenticated-orcid":true,"given":"Wenxia","family":"Fang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/054x1kd82","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Biological Sciences and Technology, Guangxi Academy of Sciences","place":["Nanning, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myad129"},{"key":"e_1_3_3_3_2","unstructured":"WHO. 2022. 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