{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T15:34:25Z","timestamp":1790091265021,"version":"4.0.1"},"reference-count":71,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T00:00:00Z","timestamp":1756771200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T00:00:00Z","timestamp":1756771200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,9,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Vaginal dysbiosis is linked to recurrent infections and reproductive complications. Probiotics may restore vaginal microbiota, but there is modest evidence to support vaginal colonization after oral administration. This work aimed to screen a vaginal lactobacilli collection (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 45) and assess vaginal colonization of selected candidates.\n                      <jats:italic toggle=\"yes\">Lactobacillus gasseri<\/jats:italic>\n                      CECT 30648 (Lg) and\n                      <jats:italic toggle=\"yes\">Lactobacillus crispatus<\/jats:italic>\n                      CECT 30647 (Lc) showed excellent\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      attributes, including antagonism against 10 urogenital pathogens, resistance to gastrointestinal tract and vaginal environment\u2014including high concentrations of biogenic amines\u2014and adhesion to vaginal epithelium. Forty-eight healthy women (18\u201345 years old) were randomly allocated to consume daily a capsule containing Lg (10\n                      <jats:sup>9<\/jats:sup>\n                      CFU), a combination of Lg plus Lc (1.5 \u00d7 10\n                      <jats:sup>9<\/jats:sup>\n                      CFU), or placebo for up to 18 days (between menses). Volunteers collected vaginal samples every 3 days. The primary endpoint was the presence of probiotic strains in vaginal swabs confirmed by strain-specific quantitative PCR. Secondary variables included vaginal microbiota composition and tolerability, among others. Probiotics were well tolerated. Lg but not Lc was detected in 55.9% of participants in active groups (9 in Lg and 10 in Lg + Lc) throughout the study (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.005 aggregated probiotic groups vs placebo). The sum of relative abundances of non-lactobacilli genera was significantly reduced in the probiotic group after intervention (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.047 vs day 0), and there was a positive transition toward lactobacilli-dominated community state types (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.039 vs day 0). Overall, we show that\n                      <jats:italic toggle=\"yes\">L. gasseri<\/jats:italic>\n                      CECT 30648 exerts a broad-spectrum antimicrobial activity and can colonize the vaginal tract of healthy women, thus showing potential to promote vaginal health. The trial was registered in clinicaltrials.gov (\n                      <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"uri\" xlink:href=\"https:\/\/clinicaltrials.gov\/study\/NCT05688397\" xlink:type=\"simple\">NCT05688397<\/jats:ext-link>\n                      ).\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        The use of probiotics to promote vaginal health is increasing because vaginal dysbiosis has been linked to numerous gynecological and reproductive complications. While vaginal administration of probiotics using devices or creams has been widely investigated, there is limited evidence supporting vaginal colonization of a probiotic administered orally. It is therefore imperative to perform extensive\n                        <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                        characterization to select a vaginal probiotic that can survive the gastrointestinal transit and effectively colonize the vaginal tract of consumers through the oral-gut-vaginal route. We have identified the strain\n                        <jats:italic toggle=\"yes\">L. gasseri<\/jats:italic>\n                        CECT 30648, which shows great probiotic properties, including antagonism against several relevant urogenital pathogens, can colonize the vaginal tract of &gt;55% of participants, and can modulate vaginal microbiota toward a lactobacillus-dominated status in a randomized controlled clinical trial in healthy premenopausal women. These results suggest that oral consumption of\n                        <jats:italic toggle=\"yes\">L. gasseri<\/jats:italic>\n                        CECT 30648 might be effective in promoting vaginal health.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.00211-25","type":"journal-article","created":{"date-parts":[[2025,8,7]],"date-time":"2025-08-07T14:34:31Z","timestamp":1754577271000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["<i>Lactobacillus gasseri<\/i>\n                    CECT 30648 shows probiotic characteristics and colonizes the vagina of healthy women after oral administration"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1059-3677","authenticated-orcid":false,"given":"Marta","family":"Perez","sequence":"first","affiliation":[{"name":"R&D Department, AB-Biotics S.A. (Part of Kaneka Corporation)","place":["Barcelona, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Armengol","sequence":"additional","affiliation":[{"name":"R&D Department, AB-Biotics S.A. (Part of Kaneka Corporation)","place":["Barcelona, Spain"]},{"name":"Basic Sciences Department, Universitat Internacional de Catalunya","place":["Barcelona, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Del Casale","sequence":"additional","affiliation":[{"name":"Open Innovation Department, MICROBION srl","place":["Verona, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilenia","family":"Campedelli","sequence":"additional","affiliation":[{"name":"Open Innovation Department, MICROBION srl","place":["Verona, Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Aldea-Perona","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04n0g0b29","id-type":"ROR","asserted-by":"publisher"}],"name":"Clinical Pharmacology, Hospital del Mar","place":["Barcelona, Spain"]},{"id":[{"id":"https:\/\/ror.org\/03a8gac78","id-type":"ROR","asserted-by":"publisher"}],"name":"Hospital del Mar Medical Research Institute","place":["Barcelona, Spain"]},{"id":[{"id":"https:\/\/ror.org\/04n0g0b29","id-type":"ROR","asserted-by":"publisher"}],"name":"Universitat Pompeu Fabra","place":["Barcelona, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"P\u00e9rez Otero","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04n0g0b29","id-type":"ROR","asserted-by":"publisher"}],"name":"Clinical Pharmacology, Hospital del Mar","place":["Barcelona, Spain"]},{"id":[{"id":"https:\/\/ror.org\/03a8gac78","id-type":"ROR","asserted-by":"publisher"}],"name":"Hospital del Mar Medical Research Institute","place":["Barcelona, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Rodriguez-Palmero","sequence":"additional","affiliation":[{"name":"R&D Department, AB-Biotics S.A. (Part of Kaneka Corporation)","place":["Barcelona, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordi","family":"Espadaler-Mazo","sequence":"additional","affiliation":[{"name":"R&D Department, AB-Biotics S.A. (Part of Kaneka Corporation)","place":["Barcelona, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2933-0586","authenticated-orcid":true,"given":"Pol","family":"Huedo","sequence":"additional","affiliation":[{"name":"R&D Department, AB-Biotics S.A. (Part of Kaneka Corporation)","place":["Barcelona, Spain"]},{"name":"Basic Sciences Department, Universitat Internacional de Catalunya","place":["Barcelona, Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1002611107"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-020-00934-6"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.819958"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-022-01083-2"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-023-01500-0"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1080\/1040841X.2022.2049696"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1097\/LGT.0000000000000644"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000000620"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.643422"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.3205\/id000046"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.3390\/children10050907"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12978-021-01292-8"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.838405"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1232825"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2014.66"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.26355\/eurrev_202201_27752"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/1471-0528.15910"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2001.tb01549.x"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1153894"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/1471-0528.15675"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajogmf.2024.101322"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13668-023-00462-3"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsyr\/foz008"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.741391"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.11.4026-4031.2001"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2013.01.071"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2016.06.031"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00297-08"},{"key":"e_1_3_5_30_2","unstructured":"Aureli P Fiore A Scalfaro C Franciosa G. 2008. Metodi microbiologici tradizionali e metodi molecolari per l\u2019analisi degli integratori alimentari a base di o con probiotici per uso umano.Rapp ISTISAN. Istituto Superiore Di Sanit\u00e0. Rome Italy"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/mra.00794-24"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky383"},{"key":"e_1_3_5_33_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms4030034"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msab293"},{"key":"e_1_3_5_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac993"},{"key":"e_1_3_5_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_5_37_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-528-2_2"},{"key":"e_1_3_5_38_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00404-015-3711-4"},{"key":"e_1_3_5_39_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00404-018-4771-z"},{"key":"e_1_3_5_40_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008841022998"},{"key":"e_1_3_5_41_2","doi-asserted-by":"crossref","unstructured":"Ruiz MA Pardo A Rejas J Soto J Villasante F Aranguren JL. 2008. Development and validation of the \u201cTreatment satisfaction with medicines questionnaire\u201d (SATMED-Q)\u00a9. value heal. Available from: https:\/\/pubmed.ncbi.nlm.nih.gov\/18494753\/","DOI":"10.1111\/j.1524-4733.2008.00323.x"},{"key":"e_1_3_5_42_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1224910"},{"key":"e_1_3_5_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2010.133"},{"key":"e_1_3_5_44_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm864"},{"key":"e_1_3_5_45_2","article-title":"Scikit-learn: machine learning in python","author":"Pedregosa F","year":"2011","unstructured":"Pedregosa F, Varoquaux G, Gramfort A, Michel V, Thirion B, Grisel O, et al.. 2011. Scikit-learn: machine learning in python. J Mach Learn Res","journal-title":"J Mach Learn Res"},{"key":"e_1_3_5_46_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-020-03803-z"},{"key":"e_1_3_5_47_2","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v034.i02"},{"key":"e_1_3_5_48_2","doi-asserted-by":"publisher","DOI":"10.3390\/jcm10071461"},{"key":"e_1_3_5_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02286-07"},{"key":"e_1_3_5_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0958-6946(99)00024-2"},{"key":"e_1_3_5_51_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1125239"},{"key":"e_1_3_5_52_2","doi-asserted-by":"publisher","DOI":"10.3920\/BM2022.0091"},{"key":"e_1_3_5_53_2","doi-asserted-by":"publisher","unstructured":"Dellino M Daniele A Vagno GD Malvasi A Zaccaro R Zaccaro K Cazzato G Cascardi E Scacco S Tinelli R De Luca A Giacomino ME De Nicol\u00f2 M Vinciguerra M Loizzi V Cicinelli E Cormio G Pinto V. 2022. The Lactobacillus crispatus M247 oral administration: it really is an effective strategy in the management of papillomavirus-infected women? Infect Agent Cancer. doi:10.21203\/rs.3.rs-1480641\/v1","DOI":"10.21203\/rs.3.rs-1480641\/v1"},{"key":"e_1_3_5_54_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.03146"},{"key":"e_1_3_5_55_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26154538"},{"key":"e_1_3_5_56_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12934-021-01563-w"},{"key":"e_1_3_5_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2024.114078"},{"key":"e_1_3_5_58_2","doi-asserted-by":"publisher","DOI":"10.23736\/S2724-606X.22.05087-4"},{"key":"e_1_3_5_59_2","doi-asserted-by":"publisher","DOI":"10.1111\/apm.13261"},{"key":"e_1_3_5_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2017.08.003"},{"key":"e_1_3_5_61_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsec\/fiaa084"},{"key":"e_1_3_5_62_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.669901"},{"key":"e_1_3_5_63_2","doi-asserted-by":"publisher","DOI":"10.3945\/an.114.007229"},{"key":"e_1_3_5_64_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00558-07"},{"key":"e_1_3_5_65_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3003605"},{"key":"e_1_3_5_66_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2025.114007"},{"key":"e_1_3_5_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2019.03.011"},{"key":"e_1_3_5_68_2","doi-asserted-by":"publisher","unstructured":"Vinerbi E Chillotti F Maschio A Lenarduzzi S Camarda S Crobu F Zhernakova DV Faro VL Vriz GB Incollu S Spreckels J Kuzub N Kadric A Gacesa R Zhernakova A De Seta F Mazz\u00e0 D Busonero F Ferrando ML Girotto G Sanna S. 2025. Vaginal microbiota changes during a natural menstrual cycle in the Women4Health cohort highlights specific features of the Italian population. Microbiology. doi:10.1101\/2025.03.12.642767","DOI":"10.1101\/2025.03.12.642767"},{"key":"e_1_3_5_69_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-024-01870-5"},{"key":"e_1_3_5_70_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-015-0351-6"},{"key":"e_1_3_5_71_2","doi-asserted-by":"publisher","DOI":"10.3390\/nu12041141"},{"key":"e_1_3_5_72_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11020499"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00211-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00211-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T13:04:27Z","timestamp":1756818267000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.00211-25"}},"subtitle":[],"editor":[{"given":"Eva","family":"Pericolini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,9,2]]},"references-count":71,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,9,2]]}},"alternative-id":["10.1128\/spectrum.00211-25"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.00211-25","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,2]]},"assertion":[{"value":"2025-01-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00211-25"}}