{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T13:19:03Z","timestamp":1753881543271,"version":"3.41.2"},"reference-count":58,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T00:00:00Z","timestamp":1727913600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T00:00:00Z","timestamp":1727913600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"USDA | National Institute of Food and Agriculture","award":["2018-67017-27632"],"award-info":[{"award-number":["2018-67017-27632"]}]},{"name":"USDA | National Institute of Food and Agriculture","award":["2021-67012-35154"],"award-info":[{"award-number":["2021-67012-35154"]}]},{"name":"USDA | National Institute of Food and Agriculture","award":["2023-67011-40521"],"award-info":[{"award-number":["2023-67011-40521"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,10,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic>Salmonella enterica<\/jats:italic>\n              serotype Cerro (\n              <jats:italic>S<\/jats:italic>\n              . Cerro) is an emerging\n              <jats:italic>Salmonella<\/jats:italic>\n              serotype isolated from cattle, but the association of\n              <jats:italic>S<\/jats:italic>\n              . Cerro with disease is not well understood. While comparative genomic analyses of bovine\n              <jats:italic>S<\/jats:italic>\n              . Cerro isolates have indicated mutations in elements associated with virulence, the correlation of\n              <jats:italic>S<\/jats:italic>\n              . Cerro fecal shedding with clinical disease in cattle varies between epidemiologic studies. The primary objective of this study was to characterize the infection-relevant phenotypes of\n              <jats:italic>S<\/jats:italic>\n              . Cerro fecal isolates obtained from neonatal calves born on a dairy farm in Wisconsin, USA. The\n              <jats:italic>S<\/jats:italic>\n              . Cerro isolates varied in biofilm production and sensitivity to the bile salt deoxycholate. All\n              <jats:italic>S<\/jats:italic>\n              . Cerro isolates were sensitive to sodium hypochlorite, hydrogen peroxide, and acidic shock. However,\n              <jats:italic>S<\/jats:italic>\n              . Cerro isolates were resistant to nitric oxide stress. Two\n              <jats:italic>S<\/jats:italic>\n              . Cerro isolates were unable to compete with\n              <jats:italic>S<\/jats:italic>\n              . Typhimurium during infection of calf ligated intestinal loops, indicating decreased fitness\n              <jats:italic>in vivo<\/jats:italic>\n              . Together, our data suggest that\n              <jats:italic>S<\/jats:italic>\n              . Cerro is sensitive to some innate antimicrobial defenses present in the gut, many of which are also used to control\n              <jats:italic>Salmonella<\/jats:italic>\n              in the environment. The observed phenotypic variation in\n              <jats:italic>S<\/jats:italic>\n              . Cerro isolates from a single farm suggest phenotypic plasticity that could impact infectious potential, transmission, and persistence on a farm.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                <jats:italic>Salmonella enterica<\/jats:italic>\n                is a zoonotic pathogen that threatens both human and animal health.\n                <jats:italic>Salmonella enterica<\/jats:italic>\n                serotype Cerro is being isolated from cattle at increasing frequency over the past two decades; however, its association with clinical disease is unclear. The goal of this study was to characterize infection-relevant phenotypes of\n                <jats:italic>S<\/jats:italic>\n                . Cerro isolates obtained from dairy calves from a single farm. Our work shows that there can be variation among temporally related\n                <jats:italic>S<\/jats:italic>\n                . Cerro isolates and that these isolates are sensitive to killing by toxic compounds of the innate immune system and those used for environmental control of\n                <jats:italic>Salmonella<\/jats:italic>\n                . This work contributes to our understanding of the pathogenic potential of the emerging pathogen\n                <jats:italic>S<\/jats:italic>\n                . Cerro.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.00212-24","type":"journal-article","created":{"date-parts":[[2024,8,15]],"date-time":"2024-08-15T13:01:17Z","timestamp":1723726877000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Sensitivity of dairy calf\n            <i>Salmonella enterica<\/i>\n            serotype Cerro isolates to infection-relevant stressors"],"prefix":"10.1128","volume":"12","author":[{"given":"Sarah M.","family":"Raabis","sequence":"first","affiliation":[{"name":"Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, Wisconsin, USA"},{"name":"Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Trina 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