{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T21:20:15Z","timestamp":1793049615937,"version":"4.5.0"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T00:00:00Z","timestamp":1719878400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T00:00:00Z","timestamp":1719878400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100010804","name":"Faculty of Medicine, Prince of Songkla University","doi-asserted-by":"crossref","award":["63-557-4-7"],"award-info":[{"award-number":["63-557-4-7"]}],"id":[{"id":"10.13039\/501100010804","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,7,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Broad-spectrum ampicillin-resistant and third-generation cephalosporin-resistant Enterobacteriaceae, particularly\n              <jats:italic>Escherichia coli<\/jats:italic>\n              and\n              <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n              that have pathological features in humans, have become a global concern. This study aimed to investigate the prevalence, antimicrobial susceptibility, and molecular genetic features of extended-spectrum beta-lactamase (ESBL)-producing\n              <jats:italic>E. coli<\/jats:italic>\n              and\n              <jats:italic>K. pneumoniae<\/jats:italic>\n              isolates in Southern Thailand. Between January and August 2021, samples (\n              <jats:italic>n<\/jats:italic>\n              = 199) were collected from a tertiary care hospital in Southern Thailand. ESBL and AmpC-lactamase genes were identified using multiplex polymerase chain reaction (PCR). The genetic relationship between ESBL-producing\n              <jats:italic>E. coli<\/jats:italic>\n              and\n              <jats:italic>K. pneumoniae<\/jats:italic>\n              was determined using the enterobacterial repetitive intergenic consensus (ERIC) polymerase chain reaction. ESBL-producing\n              <jats:italic>E. coli<\/jats:italic>\n              and\n              <jats:italic>K. pneumoniae<\/jats:italic>\n              isolates were mostly collected from catheter urine samples of infected female patients. The ESBL production prevalence was highest in the medical wards (\n              <jats:italic>n<\/jats:italic>\n              = 75, 37.7%), followed by that in surgical wards (\n              <jats:italic>n<\/jats:italic>\n              = 64, 32.2%) and operating rooms (\n              <jats:italic>n<\/jats:italic>\n              = 19, 9.5%). Antimicrobial susceptibility analysis revealed that all isolates were resistant to ampicillin, cefotaxime, ceftazidime, ceftriaxone, and cefuroxime; 79.4% were resistant to ciprofloxacin; and 64.3% were resistant to trimethoprim-sulfamethoxazole. In ESBL-producing\n              <jats:italic>K. pneumoniae<\/jats:italic>\n              and\n              <jats:italic>E. coli<\/jats:italic>\n              ,\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>TEM<\/jats:sub>\n              (\n              <jats:italic>n<\/jats:italic>\n              = 57, 72.2%) and\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>CTX-M<\/jats:sub>\n              (\n              <jats:italic>n<\/jats:italic>\n              = 61, 50.8%) genes were prominent; however, no\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>VEB<\/jats:sub>\n              ,\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>GES<\/jats:sub>\n              , or\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>PER<\/jats:sub>\n              were found in any of these isolates. Furthermore, only ESBL-producing\n              <jats:italic>K. pneumoniae<\/jats:italic>\n              had co-harbored\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>TEM<\/jats:sub>\n              and\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>SHV<\/jats:sub>\n              genes at 11.6%. The ERIC-PCR pattern of multidrug-resistant ESBL-producing strains demonstrated that the isolates were clonally related (95%). Notably, the presence of multidrug-resistant and extremely resistant ESBL producers was 83.4% and 16.6%, respectively. This study highlights the presence of\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>TEM<\/jats:sub>\n              ,\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>CTX-M<\/jats:sub>\n              , and co-harbored genes in ESBL-producing bacterial isolates from hospitalized patients, which are associated with considerable resistance to beta-lactamase and third-generation cephalosporins.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>We advocate for evidence-based guidelines and antimicrobial stewardship programs to encourage rational and appropriate antibiotic use, ultimately reducing the selection pressure for drug-resistant bacteria and lowering the likelihood of ESBL-producing bacterial infections.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.00213-24","type":"journal-article","created":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T13:19:47Z","timestamp":1716988787000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Extended-spectrum beta-lactamase-producing\n            <i>Escherichia coli<\/i>\n            and\n            <i>Klebsiella pneumoniae<\/i>\n            : insights from a tertiary hospital in Southern Thailand"],"prefix":"10.1128","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1403-1628","authenticated-orcid":false,"given":"Chonticha","family":"Romyasamit","sequence":"first","affiliation":[{"name":"School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6992-033X","authenticated-orcid":false,"given":"Phoomjai","family":"Sornsenee","sequence":"additional","affiliation":[{"name":"Department of Family Medicine and Preventive Medicine, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0122-796X","authenticated-orcid":false,"given":"Soontara","family":"Kawila","sequence":"additional","affiliation":[{"name":"Microbiology Unit, Department of Pathology, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9982-1524","authenticated-orcid":true,"given":"Phanvasri","family":"Saengsuwan","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-7020-6285-8.00180-5"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.4.589"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph10126235"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.4172\/1948-5948.1000377"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.4103\/0974-777X.68531"},{"key":"e_1_3_5_7_2","unstructured":"Prevention CfDCa. 2019. 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