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Sodium houttuyfonate (SH) and sodium new houttuyfonate (SNH) are stable derivatives of houttuynin (decyl aldehyde) extracted from\n                      <jats:italic toggle=\"yes\">Houttuynia cordata<\/jats:italic>\n                      , both possessing antifungal and antibacterial pharmacological activities. However, the inhibitory effects of SH and SNH on\n                      <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                      remain unclear. Therefore, this study aims to evaluate the potential activity and possible mechanisms of SH and SNH as antifungal agents against\n                      <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                      . First, our results showed that SH and SNH exhibit significantly inhibitory activity against fluconazole-resistant\n                      <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                      strains, but do not possess effective fungicidal activity. In addition, transcriptome and RT-qPCR studies revealed that SH and SNH can repress the expression of genes related to adhesion, aggregation, and biofilm formation. Next, we observed that SH and SNH can disrupt the adhesion and aggregation of early-stage\n                      <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                      . Furthermore, using the XTT assay, crystal violet staining, and confocal laser scanning microscopy, we found that the biofilm formation ability of\n                      <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                      was disrupted by SH and SNH. We also found that SH and SNH can potentially increase chitin content and expose \u03b2-1,3-glucan in the cell wall. Finally, infection models using\n                      <jats:italic toggle=\"yes\">Galleria mellonella<\/jats:italic>\n                      larvae and mice with systemic candidiasis demonstrated that SH and SNH significantly inhibited the colonization and pathological damage of\n                      <jats:italic toggle=\"yes\">C. auris in vivo<\/jats:italic>\n                      . Therefore, our presented results suggest that SH and SNH can effectively inhibit the growth, adhesion, aggregation, and biofilm formation to treat its colonization and pathological damage to the host of\n                      <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      Recently, the annual proportion of non-\n                      <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                      infections has been rising. The most notable characteristic of\n                      <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                      is its resistance to drugs, including multidrug resistance, which results in treatment failures and poses significant challenges in controlling its spread. Sodium houttuyfonate (SH) and sodium new houttuyfonate (SNH) are effective and stable derivatives of houttuynin (decyl aldehyde) extracted from traditional Chinese herbal medicine Houttuynia cordata, both possessing antifungal and antibacterial pharmacological activities. However, the inhibitory effects of SH and SNH on\n                      <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                      remain unclear. Through\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                      approaches, we have demonstrated that SH and SNH can effectively inhibit the growth, adhesion, aggregation, and biofilm formation to treat its colonization and pathological damage to the host of\n                      <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                      . Thus, our findings provide new insights into possible options for clinical applications in the anti-\n                      <jats:italic toggle=\"yes\">C. auris<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.00222-25","type":"journal-article","created":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T07:22:43Z","timestamp":1750231363000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["<i>In vitro<\/i>\n                    and\n                    <i>in vivo<\/i>\n                    activity of sodium houttuyfonate and sodium new houttuyfonate against\n                    <i>Candida auris<\/i>\n                    infection by affecting adhesion, aggregation, and biofilm formation 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