{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T01:18:42Z","timestamp":1785460722778,"version":"3.56.0"},"reference-count":99,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2021,10,31]],"date-time":"2021-10-31T00:00:00Z","timestamp":1635638400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,10,31]],"date-time":"2021-10-31T00:00:00Z","timestamp":1635638400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2021,10,31]]},"abstract":"<jats:p>Previous evidence showed that intrahost genetic diversity in arboviruses may be linked to viral pathogenesis and that one or a few amino acid replacements within a single protein are enough to modify a biological feature of an RNA virus. To assess 2 dengue virus determinants potentially involved in pathogenesis, we previously analyzed the intrahost genetic diversity of the virus in patients with different clinical outcomes and identified a set of 141 mutations that deserved further study. Thus, through a molecular modeling approach, we showed that disruptive variants were identified primarily among cases with mild dengue fever, while potential immune-escape variants were mainly associated with cases of greater severity.<\/jats:p>","DOI":"10.1128\/spectrum.00256-21","type":"journal-article","created":{"date-parts":[[2021,9,1]],"date-time":"2021-09-01T14:31:09Z","timestamp":1630506669000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["<i>In Silico<\/i>Analysis of Dengue Virus Serotype 2 Mutations Detected at the Intrahost Level in Patients with Different Clinical Outcomes"],"prefix":"10.1128","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8955-8972","authenticated-orcid":true,"given":"Maria Celeste","family":"Torres","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Flaviv\u00edrus, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Luiza","family":"Martins Karl","sequence":"additional","affiliation":[{"name":"Grupo de Modelagem Molecular de Sistemas Biol\u00f3gicos, Laborat\u00f3rio Nacional de Computa\u00e7\u00e3o Cient\u00edfica, GMMSB\/LNCC-MCTI, Petr\u00f3polis, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matheus","family":"M\u00fcller Pereira da Silva","sequence":"additional","affiliation":[{"name":"Grupo de Modelagem Molecular de Sistemas Biol\u00f3gicos, Laborat\u00f3rio Nacional de Computa\u00e7\u00e3o Cient\u00edfica, GMMSB\/LNCC-MCTI, Petr\u00f3polis, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent Emmanuel","family":"Dardenne","sequence":"additional","affiliation":[{"name":"Grupo de Modelagem Molecular de Sistemas Biol\u00f3gicos, Laborat\u00f3rio Nacional de Computa\u00e7\u00e3o Cient\u00edfica, GMMSB\/LNCC-MCTI, Petr\u00f3polis, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Maria","family":"Bispo de Filippis","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Flaviv\u00edrus, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","unstructured":"World Health Organization. 2009. Dengue guidelines for diagnosis treatment prevention and control. https:\/\/apps.who.int\/iris\/handle\/10665\/44188. Accessed 17 June 2019."},{"key":"e_1_3_3_3_2","first-page":"1101","volume-title":"Fields virology","author":"Lindenbarch BD","year":"2007","unstructured":"Lindenbarch BD, Thiel HJ, Rice CM. 2007. Flaviviridae: their viruses and their replication, p 1101\u20131152. In Knipe DM, Howley PM (ed), Fields virology, 5th ed. Lippincott-Raven Publishers, Philadelphia, PA, USA.","edition":"5"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01788-07"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2018.04.008"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02704-06"},{"key":"e_1_3_3_7_2","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1093\/oso\/9780199211128.003.0004","volume-title":"The evolution and emergence of RNA viruses. Oxford Series in Ecology and Evolution","author":"Holmes E","year":"2009","unstructured":"Holmes E. 2009. The RNA virus quasispecies, p 87\u2013103. In Harvey PH, May RM (ed), The evolution and emergence of RNA viruses. Oxford Series in Ecology and Evolution. Oxford University Press, Oxford, UK."},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.11.028"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2008.10.009"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.2174\/092986610791498984"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.2006459"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1000240"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1086\/518895"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-019-0465-y"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0030201"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1232294100"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.610428"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005277"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00206-16"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.3390\/v13020349"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.csbj.2020.11.020"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature04388"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2017.88"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0305892101"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2003056117"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000632"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1153263"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01805-15"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(01)00515-1"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-015-6538-9"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0832193100"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.9.4268-4275.2001"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2004.06.019"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.070813-0"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1200566109"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature14130"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2018.00621"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1247749"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1322036111"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.090936"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M500588200"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20051767"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1805195115"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002934"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-020-0312-x"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.20.9633-9643.2001"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0012836"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2010.06.039"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/21.11.2757"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1514978112"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004682"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.3390\/v9040091"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01239-15"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02283-06"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M204977200"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02343-10"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02483-10"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02424-12"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2015.06.010"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2017.10.016"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201606609"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02882-14"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01836-16"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1021\/jp504911r"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.03011-14"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1302675"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0090704"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12985-015-0456-4"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01900-12"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00546-15"},{"key":"e_1_3_3_71_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.03453-14"},{"key":"e_1_3_3_72_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00553-15"},{"key":"e_1_3_3_73_2","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.001462"},{"key":"e_1_3_3_74_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.03454-14"},{"key":"e_1_3_3_75_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02774-12"},{"key":"e_1_3_3_76_2","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.1835"},{"key":"e_1_3_3_77_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01647-08"},{"key":"e_1_3_3_78_2","doi-asserted-by":"publisher","DOI":"10.3390\/v3091739"},{"key":"e_1_3_3_79_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004604"},{"key":"e_1_3_3_80_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.9.7510-7522.1998"},{"key":"e_1_3_3_81_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1444206"},{"key":"e_1_3_3_82_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02677-14"},{"key":"e_1_3_3_83_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02506-18"},{"key":"e_1_3_3_84_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006265"},{"key":"e_1_3_3_85_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.4.2309-2324.2005"},{"key":"e_1_3_3_86_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2012.10.008"},{"key":"e_1_3_3_87_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003172"},{"key":"e_1_3_3_88_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003412"},{"key":"e_1_3_3_89_2","doi-asserted-by":"publisher","DOI":"10.1002\/cpbi.3"},{"key":"e_1_3_3_90_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3213"},{"key":"e_1_3_3_91_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1999.3091"},{"key":"e_1_3_3_92_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-10-159"},{"key":"e_1_3_3_93_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-11994-0"},{"key":"e_1_3_3_94_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889892009944"},{"key":"e_1_3_3_95_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560020916"},{"key":"e_1_3_3_96_2","doi-asserted-by":"publisher","DOI":"10.1038\/356083a0"},{"key":"e_1_3_3_97_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0046688"},{"key":"e_1_3_3_98_2","doi-asserted-by":"publisher","DOI":"10.1021\/jm051256o"},{"key":"e_1_3_3_99_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10822-013-9644-8"},{"key":"e_1_3_3_100_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0401799101"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/Spectrum.00256-21","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/Spectrum.00256-21","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,8]],"date-time":"2023-11-08T04:32:34Z","timestamp":1699417954000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/Spectrum.00256-21"}},"subtitle":[],"editor":[{"given":"Yun Young","family":"Go","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,10,31]]},"references-count":99,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,10,31]]}},"alternative-id":["10.1128\/Spectrum.00256-21"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.00256-21","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,31]]},"assertion":[{"value":"2021-05-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-08-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00256-21"}}