{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T21:24:19Z","timestamp":1773523459304,"version":"3.50.1"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T00:00:00Z","timestamp":1727913600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T00:00:00Z","timestamp":1727913600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,10,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              The minimum inhibitory concentration (MIC) of echinocandins against\n              <jats:italic>Aspergillus<\/jats:italic>\n              spp. does not represent the actual inhibition threshold of echinocandins. Therefore, the recommended method to evaluate their activity is determining the minimum effective concentration (MEC) in broth microdilution, a method that is less common in clinical settings. This study aimed to assess a user-friendly commercial method, Sensititre YeastOne (SYO), to determine the effectiveness of echinocandins (caspofungin, anidulafungin and micafungin) against\n              <jats:italic>Aspergillus<\/jats:italic>\n              spp. Echinocandins MEC was determined against 23 isolates of\n              <jats:italic>Aspergillus<\/jats:italic>\n              spp. using SYO and the reference Clinical and Laboratory Standards Institute (CLSI) method. MECs were read with an inverted microscope and a reading mirror. Essential agreement (EA) between the tested methods was defined as a \u00b1twofold dilution difference. There was a high EA (91%\u2013100%) between the reference method and SYO in determining echinocandins MEC against\n              <jats:italic>Aspergillus<\/jats:italic>\n              isolates using inverted microscopy. A high EA was also observed between SYO MEC determined by inverted microscopy and a reading mirror, but different incubation times were required. SYO is a reliable, simple method for determining the MEC of echinocandins against\n              <jats:italic>Aspergillus<\/jats:italic>\n              isolates, preferably with an inverted microscope, and can be easily used in clinical laboratories when echinocandin susceptibility testing is required.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                Using a commercial method such as Sensititre YeastOne (SYO) to determine the minimum effective concentration (MEC) of echinocandins against\n                <jats:italic>Aspergillus<\/jats:italic>\n                spp. has been shown to be a reliable alternative to the Clinical and Laboratory Standards Institute (CLSI) reference method. This makes it more suitable for high-volume clinical laboratories. SYO provides accurate results comparable to the standard method and could potentially improve patient care by guiding more optimal antifungal treatment choices for patients with\n                <jats:italic>Aspergillus<\/jats:italic>\n                infections.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.00280-24","type":"journal-article","created":{"date-parts":[[2024,8,20]],"date-time":"2024-08-20T13:05:46Z","timestamp":1724159146000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Comparative evaluation of Sensititre YeastOne and CLSI M38-Ed3 reference method for determining echinocandin minimum effective concentrations against\n            <i>Aspergillus<\/i>\n            isolates"],"prefix":"10.1128","volume":"12","author":[{"given":"Assaf","family":"Potruch","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel"},{"name":"Department of Nephrology and Hypertension, Hadassah Medical Center, Jerusalem, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hila","family":"Elinav","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel"},{"name":"Department of Microbiology and Infectious Diseases, Hadassah Medical Center, Jerusalem, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matan J.","family":"Cohen","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel"},{"name":"Clalit Health Services, Jerusalem, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Rouvinski","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada, Hebrew University-Hadassah Medical School, Jerusalem, Israel"},{"name":"The Kuvin Center for the Study of Infectious and Tropical Diseases, Hebrew University of Jerusalem, Jerusalem, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Itzhack","family":"Polacheck","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3276-0963","authenticated-orcid":true,"given":"Maya","family":"Korem","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Hebrew University of Jerusalem, Jerusalem, Israel"},{"name":"Department of Microbiology and Infectious Diseases, Hadassah Medical Center, Jerusalem, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00140-18"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/imj.15591"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw326"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009711"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00399-19"},{"key":"e_1_3_2_7_2","volume-title":"CLSI document M38Ed3","author":"CLSI","unstructured":"CLSI. n.d. Reference method for broth dilution antifungal susceptibility testing of filamentous fungi. In CLSI document M38Ed3. Clinical and Laboratory Standards Institute."},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkaa102"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00287-07"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.3906\/sag-1909-97"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.riam.2017.11.001"},{"key":"e_1_3_2_12_2","volume-title":"CLSI document M38M51S","author":"CLSI","unstructured":"CLSI. n.d. Performance standards for antifungal susceptibility testing of filamentous fungi. In CLSI document M38M51S, 3rd ed. Clinical and Laboratory Standards Institute.","edition":"3"},{"key":"e_1_3_2_13_2","volume-title":"Instructions for use: Thermo scientific Sensititre Yeastone susceptibility plates, manual, Thermo Fisher scientific Inc","year":"2015","unstructured":"2015. Instructions for use: Thermo scientific Sensititre Yeastone susceptibility plates, manual, Thermo Fisher scientific Inc. TREK Diagnostic Systems: West Sussex, UK."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.3390\/jof4010005"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00190-08"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2006.03.004"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01187-06"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.9.4358-4360.2004"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00044-17"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-009-7108-9"},{"key":"e_1_3_2_21_2","unstructured":"The European Committee on Antimicrobial Susceptibility Testing. 2020. Clinical breakpoints for fungi. Version 10.0. Available from: http;\/\/eucast.org\/astoffungi\/clinicalbreakpointsforantifungals"},{"key":"e_1_3_2_22_2","volume-title":"Epidemiological values of antifungal susceptibility testing","author":"CLSI","year":"2022","unstructured":"CLSI. 2022. M57S. In Epidemiological values of antifungal susceptibility testing. Clinical and Laboratory Standards Institutes."},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/myc.13620"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.3390\/jof7010017"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00280-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00280-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T13:10:53Z","timestamp":1727961053000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.00280-24"}},"subtitle":[],"editor":[{"given":"Paul M.","family":"Luethy","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2024,10,3]]},"references-count":23,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2024,10,3]]}},"alternative-id":["10.1128\/spectrum.00280-24"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.00280-24","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,3]]},"assertion":[{"value":"2024-01-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00280-24"}}