{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T19:14:59Z","timestamp":1787771699065,"version":"build-2784847793"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2021,10,31]],"date-time":"2021-10-31T00:00:00Z","timestamp":1635638400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,10,31]],"date-time":"2021-10-31T00:00:00Z","timestamp":1635638400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2021,10,31]]},"abstract":"<jats:p>To meet the challenges imposed by the COVID-19 pandemic, research laboratories shifted their focus and clinical diagnostic laboratories developed and utilized new assays. Nucleic acid-based testing became widespread and, for the first time, was used as a prophylactic measure.<\/jats:p>","DOI":"10.1128\/spectrum.00313-21","type":"journal-article","created":{"date-parts":[[2021,9,15]],"date-time":"2021-09-15T14:35:53Z","timestamp":1631716553000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Laboratory-Generated DNA Can Cause Anomalous Pathogen Diagnostic Test Results"],"prefix":"10.1128","volume":"9","author":[{"given":"Lindsey R.","family":"Robinson-McCarthy","sequence":"first","affiliation":[{"name":"Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander J.","family":"Mijalis","sequence":"additional","affiliation":[{"name":"Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel T.","family":"Filsinger","sequence":"additional","affiliation":[{"name":"Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helena","family":"de Puig","sequence":"additional","affiliation":[{"name":"Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA"},{"name":"Institute for Medical Engineering and Science, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nina M.","family":"Donghia","sequence":"additional","affiliation":[{"name":"Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas E.","family":"Schaus","sequence":"additional","affiliation":[{"name":"Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert A.","family":"Rasmussen","sequence":"additional","affiliation":[{"name":"Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raphael","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA"},{"name":"Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeantine E.","family":"Lunshof","sequence":"additional","affiliation":[{"name":"Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA"},{"name":"Department of Global Health and Social Medicine, Harvard Center for Bioethics, Harvard Medical School, Boston, Massachusetts, USA"},{"name":"European Research Institute for the Biology of Ageing, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George","family":"Chao","sequence":"additional","affiliation":[{"name":"Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitry","family":"Ter-Ovanesyan","sequence":"additional","affiliation":[{"name":"Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Dodd","sequence":"additional","affiliation":[{"name":"Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA"},{"name":"Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erkin","family":"Kuru","sequence":"additional","affiliation":[{"name":"Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA"},{"name":"Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adama M.","family":"Sesay","sequence":"additional","affiliation":[{"name":"Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua","family":"Rainbow","sequence":"additional","affiliation":[{"name":"Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA"},{"name":"Department of Electrical and Electronic Engineering, University of Bath, Bath, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew C.","family":"Pawlowski","sequence":"additional","affiliation":[{"name":"Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA"},{"name":"Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy M.","family":"Wannier","sequence":"additional","affiliation":[{"name":"Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolaas M.","family":"Angenent-Mari","sequence":"additional","affiliation":[{"name":"Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA"},{"name":"Institute for Medical Engineering and Science, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, USA"},{"name":"Department of Biological Engineering, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Devora","family":"Najjar","sequence":"additional","affiliation":[{"name":"Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA"},{"name":"Institute for Medical Engineering and Science, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, USA"},{"name":"MIT Media Lab, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Yin","sequence":"additional","affiliation":[{"name":"Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA"},{"name":"Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4319-6520","authenticated-orcid":true,"given":"Donald E.","family":"Ingber","sequence":"additional","affiliation":[{"name":"Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA"},{"name":"Vascular Biology Program, Department of Surgery, Boston Children\u2019s Hospital and Harvard Medical School, Boston, Massachusetts, USA"},{"name":"Harvard John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3767-7205","authenticated-orcid":true,"given":"Jenny M.","family":"Tam","sequence":"additional","affiliation":[{"name":"Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA"},{"name":"Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George M.","family":"Church","sequence":"additional","affiliation":[{"name":"Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA"},{"name":"Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, Massachusetts, USA"},{"name":"Harvard-MIT Program in Health Sciences and Technology, Cambridge, Massachusetts, USA"},{"name":"Blavatnik Institute, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41563-020-00906-z"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-020-0761-6"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0159274"},{"key":"e_1_3_2_5_2","unstructured":"Johns Hopkins University Coronavirus Resource Center. 2021. Daily state-by-state testing trends. Johns Hopkins University Baltimore MD. https:\/\/coronavirus.jhu.edu\/testing\/individual-states. Accessed June 28 2021."},{"key":"e_1_3_2_6_2","unstructured":"Pierce H McKinney R Jr. 2020. AAMC recommendations for COVID-19 testing: the current state and the way forward. Association of American Medical Colleges Washington DC. https:\/\/www.aamc.org\/covidroadmap\/testing."},{"key":"e_1_3_2_7_2","unstructured":"Centers for Disease Control and Prevention. 2020. Interim guidance on ending isolation and precautions for adults with COVID-19. Centers for Disease Control and Prevention Atlanta GA. https:\/\/www.cdc.gov\/coronavirus\/2019-ncov\/hcp\/duration-isolation.html. Accessed May 10 2021."},{"key":"e_1_3_2_8_2","unstructured":"Massachusetts Department of Public Health. 2021. Massachusetts COVID-19 interactive dashboard. Massachusetts Department of Public Health Boston MA. https:\/\/www.mass.gov\/info-details\/covid-19-response-reporting#covid-19-interactive-data-dashboard. Accessed June 28 2021."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1003346"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.7326\/M20-6976"},{"key":"e_1_3_2_11_2","unstructured":"Centers for Disease Control and Prevention. 2020. Research use only 2019-novel coronavirus (2019-nCoV) real-time RT-PCR primers and probes. Centers for Disease Control and Prevention Atlanta GA. https:\/\/www.cdc.gov\/coronavirus\/2019-ncov\/lab\/rt-pcr-panel-primer-probes.html."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.crmeth.2021.100005"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00723-21"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00225-18"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.12.e63"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coesh.2020.09.006"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1101\/2020.06.15.20117747"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2020.04.004"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000950"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(90)90145-h"},{"key":"e_1_3_2_21_2","unstructured":"WHO Global Malaria Programme. 2018. Dos and don\u2019ts for molecular testing. World Health Organization Geneva Switzerland. https:\/\/www.who.int\/teams\/global-malaria-programme\/case-management\/diagnosis\/nucleic-acid-amplification-based-diagnostics\/dos-and-don-ts-for-molecular-testing."},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.abf8873"},{"key":"e_1_3_2_23_2","unstructured":"US Food and Drug Administration. 2020. Lucira COVID-19 all-in-one test kit letter of authorization. US Food and Drug Administration Washington DC. https:\/\/www.fda.gov\/media\/143810\/download."}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/Spectrum.00313-21","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/Spectrum.00313-21","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T08:39:26Z","timestamp":1646728766000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/Spectrum.00313-21"}},"subtitle":[],"editor":[{"given":"William","family":"Lainhart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,10,31]]},"references-count":22,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,10,31]]}},"alternative-id":["10.1128\/Spectrum.00313-21"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.00313-21","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,31]]},"assertion":[{"value":"2021-05-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-08-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00313-21"}}