{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:02:27Z","timestamp":1792803747156,"version":"4.5.0"},"reference-count":55,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2023,6,15]],"date-time":"2023-06-15T00:00:00Z","timestamp":1686787200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2023,6,15]],"date-time":"2023-06-15T00:00:00Z","timestamp":1686787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2023,6,15]]},"abstract":"<jats:p>The relationship between lung microbiota and cancer has been explored based on DNA sequencing; however, culture-dependent approaches are indispensable for further studies on the lung microbiota. In this study, we applied a comprehensive approach combining culturomics and 16S rRNA gene amplicon sequencing to detect members of the microbiotas in saliva and BALF samples from patients with unilateral lobar masses.<\/jats:p>","DOI":"10.1128\/spectrum.00314-23","type":"journal-article","created":{"date-parts":[[2023,4,24]],"date-time":"2023-04-24T14:03:02Z","timestamp":1682344982000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":67,"title":["Characterization of Lung and Oral Microbiomes in Lung Cancer Patients Using Culturomics and 16S rRNA Gene Sequencing"],"prefix":"10.1128","volume":"11","author":[{"given":"Yifan","family":"Sun","sequence":"first","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuejiao","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianjie","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Thoracic Oncology, Peking University Cancer Hospital, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yafang","family":"Tan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tongtong","family":"An","sequence":"additional","affiliation":[{"name":"Department of Thoracic Oncology, Peking University Cancer Hospital, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minglei","family":"Zhuo","sequence":"additional","affiliation":[{"name":"Department of Thoracic Oncology, Peking University Cancer Hospital, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyuan","family":"Pan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Menglei","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Thoracic Oncology, Peking University Cancer Hospital, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Thoracic Oncology, Peking University Cancer Hospital, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongwei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Thoracic Oncology, Peking University Cancer Hospital, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziping","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Thoracic Oncology, Peking University Cancer Hospital, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruifu","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8309-2259","authenticated-orcid":true,"given":"Yujing","family":"Bi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(96)07492-2"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2019.05.001"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-21-1059"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.679829"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-91195-2"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2016.10.016"},{"key":"e_1_3_3_8_2","first-page":"1775","article-title":"Lung tissue microbial profile in lung cancer is distinct from emphysema","volume":"8","author":"Liu Y","year":"2018","unstructured":"Liu Y, O\u2019Brien JL, Ajami NJ, Scheurer ME, Amirian ES, Armstrong G, Tsavachidis S, Thrift AP, Jiao L, Wong MC, Smith DP, Spitz MR, Bondy ML, Petrosino JF, Kheradmand F. 2018. Lung tissue microbial profile in lung cancer is distinct from emphysema. Am J Cancer Res 8:1775\u20131787.","journal-title":"Am J Cancer Res"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.31098"},{"key":"e_1_3_3_10_2","first-page":"2047","article-title":"The characterization of lung microbiome in lung cancer patients with different clinicopathology","volume":"9","author":"Huang D","year":"2019","unstructured":"Huang D, Su X, Yuan M, Zhang S, He J, Deng Q, Qiu W, Dong H, Cai S. 2019. The characterization of lung microbiome in lung cancer patients with different clinicopathology. Am J Cancer Res 9:2047\u20132063.","journal-title":"Am J Cancer Res"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-49195-w"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2020-216385"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-20-0263"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2018.00520"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-018-0485-5"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-018-0041-0"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.crvi.2018.03.004"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms221910429"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23116138"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2022.07.006"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-016-0215-9"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02284-14"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202203115"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2016.31"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trecan.2019.12.007"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-021115-105238"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2021.1897208"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb-2018-0181"},{"key":"e_1_3_3_29_2","article-title":"Curtanaerobium respiraculi gen. nov., sp. nov., a novel anaerobic bacterium isolated from human bronchoalveolar lavage fluid","author":"Yifan Sun","year":"2023","unstructured":"Yifan Sun, Likun Wang, Dexing Han, Jianjie Li, Yuejiao Liu, Yafang Tan, Zhiyuan Pan, Ruifu Yang, Pinghua Qu, Ziping Wang, Yujing Bi. 2023. Curtanaerobium respiraculi gen. nov., sp. nov., a novel anaerobic bacterium isolated from human bronchoalveolar lavage fluid. Int J Systematic and Evolutionary Microbiology, in press.","journal-title":"Int J Systematic and Evolutionary Microbiology, in press"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1097\/RHU.0000000000001272"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-021-06058-y"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-022-02395-7"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-72132-1"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-022-02572-2"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201710-2118OC"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22063128"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.824042"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-22344-4"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40265-021-01635-6"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201705-1043SO"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/Spectrum.00802-21"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2020.06.001"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00021-19"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiph.2019.01.001"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2015.03.008"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00826"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201504-0779OC"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.12.040"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.01584"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02891"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.002516"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2016.203"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.513305"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00675-18"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00044-16"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.22301"}],"container-title":["Microbiology Spectrum"],"original-title":[],"contributor":[{"given":"Raunak","family":"Das","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"reviewer"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7600-0734","authenticated-orcid":false,"given":"Hassan","family":"Zafar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"reviewer"}]},{"given":"Pierre Le","family":"Bars","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"reviewer"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7105-0940","authenticated-orcid":false,"given":"Pablo Millones","family":"G\u00f3mez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"reviewer"}]}],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00314-23","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00314-23","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,15]],"date-time":"2023-06-15T13:30:21Z","timestamp":1686835821000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.00314-23"}},"subtitle":[],"editor":[{"given":"Diyan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2023,6,15]]},"references-count":55,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,6,15]]}},"alternative-id":["10.1128\/spectrum.00314-23"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.00314-23","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,15]]},"assertion":[{"value":"2023-01-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-04-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00314-23"}}