{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T17:31:57Z","timestamp":1791653517446,"version":"4.3.4"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T00:00:00Z","timestamp":1764633600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T00:00:00Z","timestamp":1764633600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,12,2]]},"abstract":"ABSTRACT<\/jats:title>\n \n \n \n Surgical site infections (SSIs) caused by\n Pseudomonas\n aeruginosa<\/jats:italic>\n pose significant challenges in healthcare,\n particularly in low and middle-income countries (LMICs). Understanding the\n genomic characteristics and antimicrobial resistance profiles of\n P.\n aeruginosa<\/jats:italic>\n isolates is crucial for informing appropriate\n treatment and guiding infection prevention and control strategies.\n P. aeruginosa<\/jats:italic>\n isolates recovered from SSIs were\n analyzed using whole-genome sequencing and antimicrobial susceptibility\n testing. The isolates were identified using matrix assisted laser desorption\n ionization time of flight mass spectrometry (MALDI-ToF MS) system, and their\n antibiotic susceptibilities were determined using the Kirby\u2013Bauer\n disk diffusion method and interpreted with Clinical and Laboratory Standards\n Institute guidelines. Whole-genome sequencing was performed using the\n Illumina MiSeq platform, and sequence data were analyzed using free online\n bioinformatics tools, including CARD-RGI, ResFinder, PAst, VFDB, and MLST.\n Between July 2018 and September 2023, 563 patients were enrolled in a\n surgical site infection study, which led to the recovery of 82\n P<\/jats:italic>\n .\n aeruginosa<\/jats:italic>\n isolates. The isolates\n were resistant to ciprofloxacin (\n n<\/jats:italic>\n = 23, 28.05%),\n gentamicin (\n n<\/jats:italic>\n = 20, 24.39%), piperacillin-tazobactam\n (\n n<\/jats:italic>\n = 13, 15.85%), cefepime (\n n<\/jats:italic>\n = 12,\n 14.63%), ceftazidime (\n n<\/jats:italic>\n = 12, 14.63%), meropenem\n (\n n<\/jats:italic>\n = 10, 12.20%), and amikacin (\n n<\/jats:italic>\n = 8,\n 9.76%). Multidrug resistance was noted in 10 isolates (12.20%), with three\n isolates resistant to all antibiotics tested (\n n<\/jats:italic>\n = 3, 3.7%).\n Sequence data revealed international high-risk clones: ST308 and ST773\n (\n n<\/jats:italic>\n = 5 each, 6.10%), ST244 (\n n<\/jats:italic>\n = 4,\n 4.88%), ST446, ST357, and ST654 (\n n<\/jats:italic>\n = 2 each, 2.44%), and\n ST235 (\n n<\/jats:italic>\n = 1, 1.41%). Newly identified sequence types\n ST4287, ST4288, ST4289, ST4521, ST4522, ST4523, and ST4524 were also\n detected. Serotype O11 (\n n<\/jats:italic>\n = 28, 34.15%) was the most\n prevalent, followed by O5, O6, and O4. Key virulence genes, such as\n algD<\/jats:italic>\n ,\n lasB<\/jats:italic>\n ,\n exoS<\/jats:italic>\n ,\n plcH<\/jats:italic>\n ,\n toxA<\/jats:italic>\n , and\n plcN<\/jats:italic>\n ,\n were also detected. The identification of both high-risk and novel clones,\n coupled with diverse resistance and virulence factors, underscores the need\n for continuous surveillance of circulating strains. Employing advanced\n technologies, such as whole-genome sequencing, is essential for guiding\n infection control and treatment strategies in Ghanaian hospital\n settings.\n <\/jats:p>\n \n IMPORTANCE<\/jats:title>\n \n Surgical site infections (SSIs) caused by multidrug-resistant\n Pseudomonas aeruginosa<\/jats:italic>\n are a major challenge to\n patient care, particularly in low-resource settings. This study reveals\n extensive genomic diversity, antimicrobial resistance, and virulence\n profiles among\n P. aeruginosa<\/jats:italic>\n isolates from SSIs in\n Ghanaian hospitals. The detection of globally recognized high-risk\n clones ST235, ST308, and ST773, alongside several novel sequence types,\n suggests ongoing genomic evolution and the potential for regional\n dissemination. The presence of resistance to last-line antibiotics and\n key virulence factors highlights the therapeutic and public health\n challenges in low and middle-income countries. These findings underscore\n the urgent need for genomic surveillance, strengthened infection\n prevention and control, and targeted antimicrobial stewardship programs\n to inform treatment strategies and health policies across Ghana and the\n broader African region.\n <\/jats:p>\n <\/jats:sec>\n <\/jats:sec>","DOI":"10.1128\/spectrum.00314-25","type":"journal-article","created":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T13:00:16Z","timestamp":1761829216000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Global high-risk clones and multidrug-resistant serotype strains of\n Pseudomonas aeruginosa<\/i>\n from surgical site infections in low-resource settings"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5032-8739","authenticated-orcid":true,"given":"Beverly","family":"Egyir","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01r22mr83","id-type":"ROR","asserted-by":"publisher"}],"name":"Bacteriology Department, Noguchi Memorial Institute for Medical Research, University of Ghana","place":["Accra, Ghana"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4155-3421","authenticated-orcid":true,"given":"Rhodalyn","family":"Tagoe","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01r22mr83","id-type":"ROR","asserted-by":"publisher"}],"name":"Bacteriology Department, Noguchi Memorial Institute for Medical Research, University of Ghana","place":["Accra, Ghana"]},{"name":"U.S. Naval Medical Research Unit EURAFCENT","place":["Accra, Ghana"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4491-6902","authenticated-orcid":false,"given":"Nicholas T. 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