{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T03:09:27Z","timestamp":1790392167393,"version":"4.1.0"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T00:00:00Z","timestamp":1727913600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T00:00:00Z","timestamp":1727913600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,10,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              The potential of CRISPR\/Cas systems for nucleic acid detection in novel biosensing applications is remarkable. The current clinical diagnostic detection of\n              <jats:italic>Streptococcus pyogenes<\/jats:italic>\n              (\n              <jats:italic>S. pyogenes<\/jats:italic>\n              ) is based on serological identification, culture, and PCR. We report a rapid, simple, and sensitive method for detecting and screening for\n              <jats:italic>S. pyogenes<\/jats:italic>\n              . This novel method is a promising supplemental test. After 10 min of the sample processing and 10 min of recombinase polymerase amplification, followed by 10 min of Cas12 reaction and 3 min of lateral flow biosensor (LFB) readout, a visible outcome can be observed without the need for magnification within 33 min. This platform is robust, inexpensive, and appropriate for on-site testing. A new technique for detection was created using CRISPR-Cas12a technology, which includes two measurements: a fluorescent-CRISPR-\n              <jats:italic>S. pyogenes<\/jats:italic>\n              test and a LFB-CRISPR-\n              <jats:italic>S. pyogenes<\/jats:italic>\n              test. An approach utilizing CRISPR Cas12a was developed, and the accuracy and precision of this technique were assessed. The LoD for the fluorescence-CRISPR-\n              <jats:italic>S. pyogenes<\/jats:italic>\n              assay was 1 copy\/\u03bcL, and the technique effectively differentiated\n              <jats:italic>S. pyogenes<\/jats:italic>\n              from other microorganisms. Moreover, the detection outcomes were presented in a user-friendly manner using lateral flow biosensor strips. Conclusion: A rapid and sensitive Cas12a\/crRNA assay using recombinase RPA and LFB was developed to detect\n              <jats:italic>S. pyogenes<\/jats:italic>\n              . The Cas12a\/crRNA-based assay exhibited high specificity among different bacteria strains and extremely high sensitivity. The accuracy and rapidity of this method make it a promising tool for\n              <jats:italic>S. pyogenes<\/jats:italic>\n              detection and screening.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Patients may experience a range of symptoms due to\n              <jats:italic>Streptococcus pyogenes<\/jats:italic>\n              infections, including superficial skin infections, pharyngitis, and invasive diseases in subcutaneous tissues like streptococcal toxic shock syndrome. At present, the clinical diagnostic detection of\n              <jats:italic>S. pyogenes<\/jats:italic>\n              is based on serological identification, culture, and PCR. These detection methods are time-consuming and require sophisticated equipment, making these methods challenging for routine laboratories. Thus, there is a need for a detection platform that is capable of quickly and accurately identifying\n              <jats:italic>S. pyogenes<\/jats:italic>\n              . In this study, a rapid and sensitive Cas12a\/crRNA assay using recombinase RPA and LFB was developed to detect\n              <jats:italic>S. pyogenes<\/jats:italic>\n              . The Cas12a\/crRNA-based assay exhibited high specificity among different bacteria strains and extremely high sensitivity. This method probably plays an important role for\n              <jats:italic>S. pyogenes<\/jats:italic>\n              detection and screening.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.00345-24","type":"journal-article","created":{"date-parts":[[2024,9,10]],"date-time":"2024-09-10T11:19:04Z","timestamp":1725967144000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["A specific and ultrasensitive Cas12a\/crRNA assay with recombinase polymerase amplification and lateral flow biosensor technology for the rapid detection of\n            <i>Streptococcus pyogenes<\/i>"],"prefix":"10.1128","volume":"12","author":[{"given":"Yu","family":"Cheng","sequence":"first","affiliation":[{"name":"School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, China"},{"name":"Centre for Student Innovation and Entrepreneurship, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, China"},{"name":"Grade 2022, Stomatology Major, Zhengzhou University, Zhengzhou, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiawen","family":"Lyu","sequence":"additional","affiliation":[{"name":"Medical College, Henan University of Chinese Medicine, Zhengzhou, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangfeng","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine, School of Medicine, Zhengzhou University, Zhengzhou, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Feng","sequence":"additional","affiliation":[{"name":"School of Medicine, Henan University of Chinese Medicine, Zhengzhou, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangmei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Li","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanfeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4679-4849","authenticated-orcid":true,"given":"Wenqiao","family":"Zang","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(05)70267-X"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2312.151146"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2020.104609"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00101-13"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.13.3.470"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1080\/15476286.2019.1582974"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.112408.134123"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00770"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2012.05240.x"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2009.12.004"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.835213"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12828"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1080\/14737159.2022.2109964"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/acssensors.8b01206"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsle.2005.09.032"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1080\/14737159.2022.2109964"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2008.03.026"},{"key":"e_1_3_3_19_2","first-page":"201","article-title":"Who or what is sherlock?","volume":"29","author":"Gronowski AM","year":"2018","unstructured":"Gronowski AM. 2018. 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