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K202105"],"award-info":[{"award-number":["grant NO. K202105"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,6,4]]},"abstract":"ABSTRACT<\/jats:title>\n \n \n \n Candida albicans<\/jats:italic>\n (\n C. albicans<\/jats:italic>\n ) and\n Lactiplantibacillus plantarum<\/jats:italic>\n subsp.\n plantarum<\/jats:italic>\n (\n L. plantarum<\/jats:italic>\n ) are frequently identified in various niches, but their dual-species interaction, especially with\n C. albicans<\/jats:italic>\n in yeast form, remains unclear. This study aimed to investigate the dual-species interaction of\n L. plantarum<\/jats:italic>\n and\n C. albicans<\/jats:italic>\n , including proliferation, morphology, and transcriptomes examined by selective agar plate counting, microscopy, and polymicrobial RNA-seq, respectively. Maintaining a stable and unchanged growth rate,\n L. plantarum<\/jats:italic>\n inhibited\n C. albicans<\/jats:italic>\n yeast cell proliferation but not hyphal growth. Combining optical microscopy and atomic force microscopy, cell-to-cell direct contact and co-aggregation with\n L. plantarum<\/jats:italic>\n cells surrounding\n C. albicans<\/jats:italic>\n yeast cells were observed during dual-species interaction. Reduced\n C. albicans<\/jats:italic>\n yeast cell proliferation in mixed culture was partially due to\n L. plantarum<\/jats:italic>\n cell-free culture supernatant but not the acidic environment. Upon polymicrobial transcriptomics analysis, interesting changes were identified in both\n L. plantarum<\/jats:italic>\n and\n C. albicans<\/jats:italic>\n gene expression. First, two\n L. plantarum<\/jats:italic>\n quorum-sensing systems showed contrary changes, with the activation of\n lamBDCA<\/jats:italic>\n and repression of\n luxS<\/jats:italic>\n . Second, the upregulation of stress response-related genes and downregulation of cell cycle, cell survival, and cell integrity-related pathways were identified in\n C. albicans<\/jats:italic>\n , possibly connected to the stress posed by\n L. plantarum<\/jats:italic>\n and the reduced yeast cell proliferation. Third, a large scale of pathogenesis and virulence factors were downregulated in\n C. albicans<\/jats:italic>\n , indicating the potential interruption of pathogenic activities by\n L. plantarum<\/jats:italic>\n . Fourth, partial metabolism and transport pathways were changed in\n L. plantarum<\/jats:italic>\n and\n C. albicans<\/jats:italic>\n . The information in this study might aid in understanding the behavior of\n L. plantarum<\/jats:italic>\n and\n C. albicans<\/jats:italic>\n in dual-species interaction.\n <\/jats:p>\n \n IMPORTANCE<\/jats:title>\n \n The anti-\n Candida albicans<\/jats:italic>\n activity of\n Lactiplantibacillus plantarum<\/jats:italic>\n has been explored in the past decades. However, the importance of\n C. albicans<\/jats:italic>\n yeast form and the effect of\n C. albicans<\/jats:italic>\n on\n L. plantarum<\/jats:italic>\n had also been omitted. In this study, the dual-species interaction of\n L. plantarum<\/jats:italic>\n and\n C. albicans<\/jats:italic>\n was investigated with a focus on the transcriptomes. Cell-to-cell direct contact and co-aggregation with\n L. plantarum<\/jats:italic>\n cells surrounding\n C. albicans<\/jats:italic>\n yeast cells were observed. Upon polymicrobial transcriptomics analysis, interesting changes were identified, including contrary changes in two\n L. plantarum<\/jats:italic>\n quorum-sensing systems and reduced cell survival-related pathways and pathogenesis determinants in\n C. albicans<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>\n <\/jats:sec>","DOI":"10.1128\/spectrum.00353-24","type":"journal-article","created":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T13:01:11Z","timestamp":1715173271000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Differential alteration in\n Lactiplantibacillus plantarum<\/i>\n subsp.\n plantarum<\/i>\n quorum-sensing systems and reduced\n Candida albicans<\/i>\n yeast survival and virulence gene expression in dual-species 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South China University of Technology, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aijuan","family":"Xu","sequence":"additional","affiliation":[{"name":"Guangzhou Hybribio Medical Laboratory, Guangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Xue","sequence":"additional","affiliation":[{"name":"Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Engineering Research Center of Starch and Vegetable Protein Processing Ministry of Education, School of Food Science and Engineering, South China University of Technology, Guangzhou, China"},{"name":"Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, China, Guangzhou, Guangdong"},{"name":"Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and 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