{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T18:20:20Z","timestamp":1782238820544,"version":"3.54.5"},"reference-count":16,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T00:00:00Z","timestamp":1717459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T00:00:00Z","timestamp":1717459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"National Science and Technology Council","award":["112-2320-B-A49-045-MY3"],"award-info":[{"award-number":["112-2320-B-A49-045-MY3"]}]},{"DOI":"10.13039\/501100004319","name":"Shin Kong Wu Ho-Su Memorial Hospital","doi-asserted-by":"crossref","award":["2024SKHAND009"],"award-info":[{"award-number":["2024SKHAND009"]}],"id":[{"id":"10.13039\/501100004319","id-type":"DOI","asserted-by":"crossref"}]},{"name":"NTUH | National Taiwan University Hospital Hsin-Chu Branch","award":["113-HCH057"],"award-info":[{"award-number":["113-HCH057"]}]},{"name":"National Science and Technology Council","award":["112-2740-B-A49-002"],"award-info":[{"award-number":["112-2740-B-A49-002"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,6,4]]},"abstract":"ABSTRACT<\/jats:title>\n \n \n \n Integration of carbapenemase gene\n bla<\/jats:italic>\n IMP<\/jats:sub>\n into the chromosome of carbapenem-resistant\n Acinetobacter baumannii<\/jats:italic>\n (CRAB) has not been reported. The aim of this study was to explore the genomic characteristics of CRAB AB322 isolated from a Taiwanese patient diagnosed with bacteremia in 2011, whose chromosome harbors\n bla<\/jats:italic>\n IMP-19<\/jats:sub>\n . Disk diffusion and broth microdilution were employed to analyze the antimicrobial susceptibility of AB322 to 14 antimicrobials. Nanopore whole-genome sequencing platform was utilized for AB322 genome sequencing, and conjugation was further performed to investigate the transferability of\n bla<\/jats:italic>\n IMP-19<\/jats:sub>\n to amikacin-resistant\n A. baumannii<\/jats:italic>\n 218 (AB218) and\n Acinetobacter nosocomialis<\/jats:italic>\n 254 (AN254). The results showed that AB322 was classified as multidrug-resistant\n A. baumannii<\/jats:italic>\n but remained susceptible to ampicillin\/sulbactam, colistin, and tigecycline. Whole-genome sequencing revealed the AB322 genome, consisting of a 4,098,985-bp chromosome, a 71,590-bp conjugative plasmid named pAB322-1, and an 8,726-bp plasmid named pAB322-2. Multilocus sequence typing analysis indicated that AB322 belonged to sequence type 1. AB322 chromosome harbored numerous acquired antimicrobial resistance genes, including\n aph(3\u2032)-Ia<\/jats:italic>\n ,\n aadA1b<\/jats:italic>\n ,\n aadA1<\/jats:italic>\n ,\n aac(6\u2032)-Ib3<\/jats:italic>\n ,\n aac (3)-Ia<\/jats:italic>\n ,\n bla<\/jats:italic>\n ADC-25<\/jats:sub>\n ,\n bla<\/jats:italic>\n OXA-69<\/jats:sub>\n ,\n bla<\/jats:italic>\n IMP-19<\/jats:sub>\n ,\n catA1<\/jats:italic>\n ,\n sul1<\/jats:italic>\n , and\n tet(A<\/jats:italic>\n ), conferring resistance to \u03b2-lactams, aminoglycosides, chloramphenicol, sulfamethoxazole, and tetracyclines. Moreover,\n bla<\/jats:italic>\n IMP-19<\/jats:sub>\n was identified to be situated within class 1 integron In240 and an incomplete PHAGE_Salmon_SJ46_NC_031129 on AB322 chromosome. However, conjugation experiments revealed that\n bla<\/jats:italic>\n IMP-19<\/jats:sub>\n could not be transferred to AB218 and AN254 in our testing conditions. In conclusion, we first report the presence of chromosomal-integrated\n bla<\/jats:italic>\n IMP-19<\/jats:sub>\n in CRAB, possibly mediated by integron. The future dissemination of\n bla<\/jats:italic>\n IMP-19<\/jats:sub>\n among different species, leading to carbapenem resistance dissemination, requires close monitoring.\n <\/jats:p>\n <\/jats:sec>\n \n IMPORTANCE<\/jats:title>\n \n The horizontal transfer of antimicrobial-resistant genes is crucial for the dissemination of resistance, especially as\n Acinetobacter baumannii<\/jats:italic>\n has emerged as a clinically significant pathogen. However, in this study, we first report the integration of the\n bla<\/jats:italic>\n IMP-19<\/jats:sub>\n gene into the chromosome of\n A. baumannii<\/jats:italic>\n , and such horizontal transfer may be associated with integron-phage elements. Additionally, it is possible that these DNA fragments carrying antimicrobial-resistant genes could further spread to other pathogens by moving horizontally onto conjugative plasmids.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1128\/spectrum.00382-24","type":"journal-article","created":{"date-parts":[[2024,4,23]],"date-time":"2024-04-23T13:01:38Z","timestamp":1713877298000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["First report of the chromosomal integration of carbapenemase gene\n bla<\/i>\n IMP-19<\/sub>\n in\n Acinetobacter baumannii<\/i>\n AB322: the legacy of integron in phage-plasmid?"],"prefix":"10.1128","volume":"12","author":[{"given":"Yung-Luen","family":"Shih","sequence":"first","affiliation":[{"name":"School of Medicine, College of Medicine, Fu-Jen Catholic University, New Taipei, Taiwan"},{"name":"Department of Pathology and Laboratory Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl Jay Ballena","family":"Bregente","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Immunology, College of Life Sciences, National Yang Ming Chiao Tung University, Taipei, Taiwan"},{"name":"College of Medical Technology, Southwestern University PHINMA, Cebu, Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pek Kee","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Immunology, College of Life Sciences, National Yang Ming Chiao Tung University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tran Thi Dieu","family":"Thuy","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Immunology, College of Life Sciences, National Yang Ming Chiao Tung University, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Chen","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han-Yueh","family":"Kuo","sequence":"additional","affiliation":[{"name":"National Taiwan University Hospital Hsin-Chu Branch, Hsin-Chu, Taiwan"},{"name":"National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsu-Feng","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, China Medical University Hospital, Taichung, Taiwan"},{"name":"Department of Medical Laboratory Science and Biotechnology, Asia University, Taichung City, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8782-3912","authenticated-orcid":true,"given":"Cheng-Yen","family":"Kao","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Immunology, College of Life Sciences, National Yang Ming Chiao Tung University, Taipei, Taiwan"},{"name":"Health Innovation Center, National Yang Ming Chiao Tung University, Taipei, Taiwan"},{"name":"Microbiota Research Center, National Yang Ming Chiao Tung University, Taipei, 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