{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T02:02:46Z","timestamp":1791424966645,"version":"4.3.3"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T00:00:00Z","timestamp":1738627200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T00:00:00Z","timestamp":1738627200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,2,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              The gastrointestinal tract is a unique ecological niche with a high abundance of various Enterobacterales in close proximity. This allows the exchange of mobile genetic elements that carry resistance determinants. In the hospital setting, resistant organisms are prevalent and selective antibiotic pressure is high, providing a supportive platform for interspecies dissemination of resistance. Seven New Delhi metallo-beta-lactamase(NDM) positive and one NDM-negative bacteria were sent for WGS followed by bioinformatics analysis. Here, we describe three separate cases of patients simultaneously colonized by two NDM producing-species (\n              <jats:italic>Escherichia coli<\/jats:italic>\n              and\n              <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n              ), in which we documented interspecies dissemination of the\n              <jats:italic>bla<\/jats:italic>\n              <jats:sub>NDM-1<\/jats:sub>\n              by an 87,450 bp IncM2 type multi-drug resistance plasmid (here named pNCICAN_NDM-01_21-il). This plasmid was found to be highly conjugative. Our results underline the risk of interspecies horizontal plasmid dissemination. Such events may cause the emergence of high-risk clones, as well as lead to under-recognized multi-species outbreaks.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>Conjugative, carbapenemase-carrying multidrug-resistant plasmids that can move between species of clinically relevant Enterobacterales pose a great risk to patients\u2019 health, especially when they spread inside a medical institution. Yet, most institutions monitor bacteria according to species and are at risk of missing plasmid-driven outbreaks. Thus, this work indicates that plasmid surveillance is an important tool for infection control.<\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.00399-24","type":"journal-article","created":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T14:47:14Z","timestamp":1734446834000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["<i>In vivo<\/i>\n            interspecies dissemination of IncM2-type\n            <i>bla<\/i>\n            <sub>NDM-1<\/sub>\n            carrying plasmid"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2488-3720","authenticated-orcid":true,"given":"Mor N.","family":"Lurie-Weinberger","sequence":"first","affiliation":[{"name":"National Institute for Antibiotic Resistance and Infection Control, Ministry of Health","place":["Tel Aviv, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-4196-7767","authenticated-orcid":true,"given":"Darya","family":"Bychenko-Banyas","sequence":"additional","affiliation":[{"name":"National Institute for Antibiotic Resistance and Infection Control, Ministry of Health","place":["Tel Aviv, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meirav","family":"Mor","sequence":"additional","affiliation":[{"name":"Schneider Children\u2019s Medical Center of Israel","place":["Petach Tikva, Israel"]},{"name":"Faculty of Medicine, Tel Aviv University","place":["Tel Aviv-Yafo, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sivan","family":"Laviad-Shitrit","sequence":"additional","affiliation":[{"name":"National Institute for Antibiotic Resistance and Infection Control, Ministry of Health","place":["Tel Aviv, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ella","family":"Kaplan","sequence":"additional","affiliation":[{"name":"National Institute for Antibiotic Resistance and Infection Control, Ministry of Health","place":["Tel Aviv, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6051-1177","authenticated-orcid":true,"given":"Nadya","family":"Rakovitsky","sequence":"additional","affiliation":[{"name":"National Institute for Antibiotic Resistance and Infection Control, Ministry of Health","place":["Tel Aviv, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alona","family":"Keren-Paz","sequence":"additional","affiliation":[{"name":"National Institute for Antibiotic Resistance and Infection Control, Ministry of Health","place":["Tel Aviv, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaim","family":"Ben-Zvi","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Tel Aviv University","place":["Tel Aviv-Yafo, Israel"]},{"name":"Microbiology Laboratory, Rabin Medical Center, Beilinson Hospital","place":["Petach Tikva, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yehuda","family":"Carmeli","sequence":"additional","affiliation":[{"name":"National Institute for Antibiotic Resistance and Infection Control, Ministry of Health","place":["Tel Aviv, Israel"]},{"name":"Faculty of Medicine, Tel Aviv University","place":["Tel Aviv-Yafo, Israel"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1306801"},{"key":"e_1_3_3_3_2","volume-title":"Hospital-acquired infectionsStatPearls","author":"Monegro AF","year":"2017","unstructured":"Monegro AF, Muppidi V, Regunath H. 2017. Hospital-acquired infectionsStatPearls. StatPearls."},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2011.00292.x"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10071292"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-021-00879-y"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1606.091671"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/35012500"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1381"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1192243"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0123063"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01707-08"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkab466"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11111641"},{"key":"e_1_3_3_15_2","volume-title":"CLSI document M100 23","author":"Clinical and Laboratory Standards Institute","year":"2023","unstructured":"Clinical and Laboratory Standards Institute. 2023. Performance standards for antimicrobial susceptibility testing. In CLSI document M100 23, 33rd ed","edition":"33"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkaa345"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02412-14"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-017-0844-4"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab902"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad326"},{"key":"e_1_3_3_21_2","volume-title":"Snippy, rapid haploid variant calling and core genome alignment","author":"Seemann T","year":"2015","unstructured":"Seemann T. 2015. Snippy, rapid haploid variant calling and core genome alignment. GitHub repository."},{"key":"e_1_3_3_22_2","volume-title":"E. coli plasmid vectors: methods and applications","author":"Nicola C","year":"2008","unstructured":"NicolaC, Andrew Preston, eds. 2008. E. coli plasmid vectors: methods and applications. 235th ed. Springer Science & Business Media.","edition":"235"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01773-10"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00399-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00399-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T14:06:33Z","timestamp":1738677993000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.00399-24"}},"subtitle":[],"editor":[{"given":"Shannon D.","family":"Manning","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,2,4]]},"references-count":22,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,2,4]]}},"alternative-id":["10.1128\/spectrum.00399-24"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.00399-24","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,4]]},"assertion":[{"value":"2024-03-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00399-24"}}