{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T14:50:11Z","timestamp":1791557411470,"version":"4.3.4"},"reference-count":60,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T00:00:00Z","timestamp":1719878400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T00:00:00Z","timestamp":1719878400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["R56AI152526"],"award-info":[{"award-number":["R56AI152526"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["RO1AI122747"],"award-info":[{"award-number":["RO1AI122747"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"name":"NIH","award":["F31 AI52286"],"award-info":[{"award-number":["F31 AI52286"]}]},{"name":"NIH","award":["T32 AI 60537"],"award-info":[{"award-number":["T32 AI 60537"]}]},{"name":"NIH","award":["T32 GM007810"],"award-info":[{"award-number":["T32 GM007810"]}]},{"name":"UCSF Discovery Fellows program"},{"name":"NIH","award":["F31 AI133951"],"award-info":[{"award-number":["F31 AI133951"]}]},{"name":"NIH","award":["F32 AI133902"],"award-info":[{"award-number":["F32 AI133902"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,7,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic>Chlamydia trachomatis<\/jats:italic>\n              is the leading cause of bacterial sexually transmitted infections in the USA and of preventable blindness worldwide. This obligate intracellular pathogen replicates within a membrane-bound inclusion, but how it acquires nutrients from the host while avoiding detection by the innate immune system is incompletely understood.\n              <jats:italic>C. trachomatis<\/jats:italic>\n              accomplishes this in part through the translocation of a unique set of effectors into the inclusion membrane, the\n              <jats:underline>inc<\/jats:underline>\n              lusion membrane proteins (Incs). Incs are ideally positioned at the host-pathogen interface to reprogram host signaling by redirecting proteins or organelles to the inclusion. Using a combination of co-affinity purification, immunofluorescence confocal imaging, and proteomics, we characterize the interaction between an early-expressed Inc of unknown function, Tri1, and tumor necrosis factor receptor-associated factor 7 (TRAF7). TRAF7 is a multi-domain protein with a RING finger ubiquitin ligase domain and a C-terminal WD40 domain. TRAF7 regulates several innate immune signaling pathways associated with\n              <jats:italic>C. trachomatis<\/jats:italic>\n              infection and is mutated in a subset of tumors. We demonstrate that Tri1 and TRAF7 specifically interact during infection and that TRAF7 is recruited to the inclusion. We further show that the predicted coiled-coil domain of Tri1 is necessary to interact with the TRAF7 WD40 domain. Finally, we demonstrate that Tri1 displaces the native TRAF7 binding partners, mitogen-activated protein kinase kinase kinase 2 (MEKK2), and MEKK3. Together, our results suggest that by displacing TRAF7 native binding partners, Tri1 has the capacity to alter TRAF7 signaling during\n              <jats:italic>C. trachomatis<\/jats:italic>\n              infection.\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                <jats:italic>Chlamydia trachomatis<\/jats:italic>\n                is the leading cause of bacterial sexually transmitted infections in the USA and preventable blindness worldwide. Although easily treated with antibiotics, the vast majority of infections are asymptomatic and therefore go untreated, leading to infertility and blindness. This obligate intracellular pathogen evades the immune response, which contributes to these outcomes. Here, we characterize the interaction between a\n                <jats:italic>C. trachomatis<\/jats:italic>\n                -secreted effector, Tri1, and a host protein involved in innate immune signaling, TRAF7. We identified host proteins that bind to TRAF7 and demonstrated that Tri1 can displace these proteins upon binding to TRAF7. Remarkably, the region of TRAF7 to which these host proteins bind is often mutated in a subset of human tumors. Our work suggests a mechanism by which Tri1 may alter TRAF7 signaling and has implications not only in the pathogenesis of\n                <jats:italic>C. trachomatis<\/jats:italic>\n                infections but also in understanding the role of TRAF7 in cancer.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.00453-24","type":"journal-article","created":{"date-parts":[[2024,5,30]],"date-time":"2024-05-30T13:03:01Z","timestamp":1717074181000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["The\n            <i>Chlamydia trachomatis<\/i>\n            Inc Tri1 interacts with TRAF7 to displace native TRAF7 interacting partners"],"prefix":"10.1128","volume":"12","author":[{"given":"Clara M.","family":"Herrera","sequence":"first","affiliation":[{"name":"Department of Medicine, University of California San Francisco, San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleanor","family":"McMahon","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California San Francisco, San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle L.","family":"Swaney","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Sherry","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California San Francisco, San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khavong","family":"Pha","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California San Francisco, San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen","family":"Adams-Boone","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California San Francisco, San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey R.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4902-337X","authenticated-orcid":false,"given":"Nevan J.","family":"Krogan","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meredith","family":"Stevers","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of California San Francisco, San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Solomon","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of California San Francisco, San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7702-3938","authenticated-orcid":false,"given":"Cherilyn","family":"Elwell","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California San Francisco, San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2168-9711","authenticated-orcid":false,"given":"Joanne","family":"Engel","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of California San Francisco, San Francisco, California, USA"},{"name":"Department of Microbiology and Immunology, University of California San Francisco, San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","volume-title":"Chlamydia","author":"Mohseni M","year":"2024","unstructured":"Mohseni M, Sung S, Takov V. 2024. Chlamydia. StatPearls Publishing, Treasure Island (FL)."},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00543-16"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2016.30"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/mmbr.00034-23"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/362104"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2000.00029.x"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1286-4579(02)01546-0"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2014.00157"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/femspd\/ftab025"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.22709"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.15698\/mic2019.09.691"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-023-00860-y"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2015.06.004"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.215426"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1086"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C400063200"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virs.2023.04.005"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-023-01675-9"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.02057.x"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01075-15"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.24011"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00104"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/jmcb\/mjad083"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.25676"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2010.04.003"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/sigtrans.2017.30"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.22311"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","unstructured":"Sherry J Dolat L McMahon E Swaney DL Bastidas RJ Johnson JR Valdivia RH Krogan NJ Elwell CA Engel JN. 2022. Chlamydia trachomatis effector Dre1 interacts with dynactin to reposition host organelles during infection. bioRxiv. doi:10.1101\/2022.04.15.488217","DOI":"10.1101\/2022.04.15.488217"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2013.10.023"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e05-08-0731"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","unstructured":"Wang J Zhong X Yin H. 2023. TRAF6 initiates inflammatory signaling via organizing membraneless cytoplasmic condensates. Immunology. doi:10.1101\/2023.06.19.545655","DOI":"10.1101\/2023.06.19.545655"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03819-2"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu700"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fuw027"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2013.12.009"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-018-0271-y"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2008.01178.x"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2016.12.002"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.03190-22"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2009.01290.x"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2023.107474"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.01.006"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3520"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-018-0127-2"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2017.153"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000001997"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.24854"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2214997120"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1233009"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-013-1093-x"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1038\/89769"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22147594"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002198"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10719"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.24.10757-10765.2004"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1511"},{"key":"e_1_3_4_59_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.252.5009.1162"},{"key":"e_1_3_4_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.03.016"},{"key":"e_1_3_4_61_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2839"},{"key":"e_1_3_4_62_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1106"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00453-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00453-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T13:08:34Z","timestamp":1719925714000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.00453-24"}},"subtitle":[],"editor":[{"given":"Brian","family":"Conlon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,7,2]]},"references-count":60,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2024,7,2]]}},"alternative-id":["10.1128\/spectrum.00453-24"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.00453-24","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,2]]},"assertion":[{"value":"2024-02-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00453-24"}}