{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T18:21:57Z","timestamp":1790446917323,"version":"4.1.0"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T00:00:00Z","timestamp":1756771200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T00:00:00Z","timestamp":1756771200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,9,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                      is an opportunistic pathogen responsible for 10% of nosocomial urinary tract infections (UTIs). Its large genome and adaptability enable it to cause a wide variety of infections, from respiratory disorders in cystic fibrosis patients to recurrent UTIs. While genomic and phenotypic adaptations of\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      have been well-studied in respiratory infections, few studies have investigated pathoadaptation in recurrent UTIs. Here, we investigated the impact of genomic alterations of sequential urinary isolates collected from three patients on phenotypic responses to environmental stresses, virulence, and motility. In addition, to gain insight on adaptive mechanisms of\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      in urine, proteomic analyses were conducted using pooled human urine compared to a standard Trypticase Soy (TS) medium. Late isolates showed significantly impaired growth and reduced responses to acid and osmotic stresses compared to early isolates, although responses to oxidative stress remained unchanged. Furthermore, the late isolates were significantly less virulent in the\n                      <jats:italic toggle=\"yes\">Galleria mellonella<\/jats:italic>\n                      infection model. Finally, proteomic analyses revealed the accumulation of proteins associated with flagellum and chemotaxis only in early isolates of two out of three patients, regardless of the culture medium. Motility assays confirmed these results, with late isolates being less motile than early ones. Moreover, siderophore-related proteins were significantly less abundant in late isolates when cultured in human urine, a result not observed in TS medium, suggesting a convergent adaptation trajectory in urine. Our results provide an initial insight into the adaptive mechanisms of\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      in the urinary tract.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                        is the third most common pathogen causing healthcare-associated UTIs. Its ability to form biofilms and develop antibiotic resistance often leads to relapses. Here, we investigated the phenotypic characteristics of longitudinal urinary isolates under specific stress conditions and used an\n                        <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                        model to evaluate the virulence of these isolates. Integrating proteomic analysis into this approach allowed us to identify the metabolic and regulatory pathways involved in bacterial adaptation and to establish innovative correlations between genomic, phenotypic, and proteomic data. Altogether, these data enabled us to map the adaptation mechanisms of\n                        <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                        in the urinary environment. These findings provide putative new therapeutic targets and contribute to our understanding of recurrent UTIs caused by this pathogen.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.00456-25","type":"journal-article","created":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T13:02:17Z","timestamp":1753794137000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Insights on pathoadaptation of sequential\n                    <i>Pseudomonas aeruginosa<\/i>\n                    isolates to the urinary tract"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-1678-0734","authenticated-orcid":true,"given":"Caroline","family":"Martin-Duval","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/051kpcy16","id-type":"ROR","asserted-by":"publisher"}],"name":"Univ Rouen Normandie, Universit\u00e9 de Caen Normandie, INSERM, Normandie Univ, DYNAMICURE UMR 1311","place":["Rouen, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4230-1886","authenticated-orcid":false,"given":"Sandrine","family":"Dahyot","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/051kpcy16","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Bacteriology, Univ Rouen Normandie, Universit\u00e9 de Caen Normandie, INSERM, Normandie Univ, DYNAMICURE UMR 1311","place":["Rouen, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"In\u00e8s","family":"Coquisart","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/051kpcy16","id-type":"ROR","asserted-by":"publisher"}],"name":"Universit\u00e9 de Caen Normandie, Univ Rouen Normandie, INSERM, Normandie Univ, DYNAMICURE UMR 1311","place":["Caen, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benoit","family":"Bernay","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/051kpcy16","id-type":"ROR","asserted-by":"publisher"}],"name":"Plateforme Proteogen SFR ICORE 4206, Universit\u00e9 de Caen Normandie","place":["Caen, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martine","family":"Pestel-Caron","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/051kpcy16","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Bacteriology, Univ Rouen Normandie, Universit\u00e9 de Caen Normandie, INSERM, Normandie Univ, DYNAMICURE UMR 1311","place":["Rouen, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8588-2732","authenticated-orcid":true,"given":"Jean-Christophe","family":"Giard","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/051kpcy16","id-type":"ROR","asserted-by":"publisher"}],"name":"Universit\u00e9 de Caen Normandie, Univ Rouen Normandie, INSERM, Normandie Univ, DYNAMICURE UMR 1311","place":["Caen, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells12010199"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsle\/fnx124"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0178178"},{"key":"e_1_3_5_5_2","unstructured":"ECDC. 2024. Point prevalence survey of healthcare-associated infections and antimicrobial use in european acute care hospitals \u2013 2022-2023. Available from: https:\/\/www.ecdc.europa.eu\/en\/publications-data\/PPS-HAI-AMR-acute-care-europe-2022-2023. Retrieved 10 Apr 2025."},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11051345"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2012.02795.x"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02359-20"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M114.043463"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009534"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.611246"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-022-12347-6"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/mmbr.00066-22"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1472-765x.1997.00378.x"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2024.1347466"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-011-0026-y"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0071845"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.02743-22"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00269-18"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb01500.x"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00101-19"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-147-6-1657"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.7.2721-2725.2006"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.59.2.473-478.1993"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.04832-22"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.7.4399-4403.2005"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02340.x"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200812-1943OC"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-23451-y"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.782733"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/2049-632X.12170"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.943346"},{"key":"e_1_3_5_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02171-20"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.81"},{"key":"e_1_3_5_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.03071-22"},{"key":"e_1_3_5_36_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0088368"},{"key":"e_1_3_5_37_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3002781"},{"key":"e_1_3_5_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.00361-23"},{"key":"e_1_3_5_39_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.9b00122"},{"key":"e_1_3_5_40_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.7b00561"},{"key":"e_1_3_5_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msae022"},{"key":"e_1_3_5_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3148"},{"key":"e_1_3_5_43_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00039"},{"key":"e_1_3_5_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2010.10.005"},{"key":"e_1_3_5_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.23.7955-7962.2005"},{"key":"e_1_3_5_46_2","first-page":"295","article-title":"Different mutations in mucA gene of Pseudomonas aeruginosa mucoid strains in cystic fibrosis patients and their effect on algU gene expression","volume":"35","author":"Pulcrano G","year":"2012","unstructured":"Pulcrano G, Iula DV, Raia V, Rossano F, Catania MR. 2012. Different mutations in mucA gene of Pseudomonas aeruginosa mucoid strains in cystic fibrosis patients and their effect on algU gene expression. New Microbiol 35:295\u2013305.","journal-title":"New Microbiol"},{"key":"e_1_3_5_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01900-14"},{"key":"e_1_3_5_48_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep41178"}],"container-title":["Microbiology Spectrum"],"original-title":[],"contributor":[{"given":"S\u00e9bastien","family":"Bontemps-Gallo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"reviewer"}]}],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00456-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00456-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T13:04:56Z","timestamp":1756818296000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.00456-25"}},"subtitle":[],"editor":[{"given":"Estela Maria","family":"Galvan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,9,2]]},"references-count":47,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,9,2]]}},"alternative-id":["10.1128\/spectrum.00456-25"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.00456-25","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,2]]},"assertion":[{"value":"2025-02-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00456-25"}}