{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T11:27:08Z","timestamp":1787657228745,"version":"build-2736575974"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T00:00:00Z","timestamp":1730764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T00:00:00Z","timestamp":1730764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["JP22fk0108132, JP22fk0108604, JP23fk0108604"],"award-info":[{"award-number":["JP22fk0108132, JP22fk0108604, JP23fk0108604"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,11,5]]},"abstract":"ABSTRACT<\/jats:title>\n \n \n \n The extended-spectrum \u03b2-lactamase (ESBL) gene,\n bla<\/jats:italic>\n VEB-1<\/jats:sub>\n , was identified for the first time in an\n Escherichia coli<\/jats:italic>\n clinical isolate, JARB-RN-0061, from blood cultures in a Japanese general hospital in 2021. The isolate exhibited high resistance to broad-spectrum cephalosporins, including ceftazidime (MIC >128 mg\/L) and cefepime (MIC = 16 mg\/L).\n bla<\/jats:italic>\n VEB-1<\/jats:sub>\n was identified during whole-genome sequencing and characterization of the isolate. JARB-RN-0061 belonged to the B2-O2:K1:H7-ST95-\n fimH<\/jats:italic>\n 41 lineage and was classified as presumptive extraintestinal pathogenic\n E. coli<\/jats:italic>\n (ExPEC) and uropathogenic\n E. coli<\/jats:italic>\n (UPEC). Moreover, the strain harbored multiple virulence genes on the chromosome. The Col156\/IncFIB(AP001918)\/IncFII(29)-type plasmid (114,216 bp), with\n clbB<\/jats:italic>\n and\n tcpC<\/jats:italic>\n genes involved in bacteremia, was unique to the\n fimH<\/jats:italic>\n 41 subclone. The\n bla<\/jats:italic>\n VEB-1<\/jats:sub>\n gene was located on a non-typeable and non-conjugative plasmid, pJARB-RN-0061_VEB-1 (17,093 bp). It was embedded in the class 1 integron In1883-like, with multidrug resistance gene cassettes for\n aacA4<\/jats:italic>\n ,\n aadB<\/jats:italic>\n ,\n cmlA5<\/jats:italic>\n ,\n qnrVC4<\/jats:italic>\n , and\n dfrA14<\/jats:italic>\n . Notably, comparative analysis of the complete sequence of plasmid pJARB-RN-0061_VEB-1 revealed that it was highly homologous to the\n bla<\/jats:italic>\n VEB-1<\/jats:sub>\n -harboring plasmid, pMS2H7VEB-1 (100% coverage and 99.99% identity), except for the Tn3 family transposon (4,931 bp) and the plasmid pRHBSTW-00138_5 (97% coverage and 100% identity) harbored by\n Klebsiella quasipneumoniae<\/jats:italic>\n subsp.\n similipneumoniae<\/jats:italic>\n strains from hospital sewage in Japan and wastewater influent in the United Kingdom, respectively. The emergence of a human pathogenic\n E. coli<\/jats:italic>\n clinical isolate with the\n bla<\/jats:italic>\n VEB-1<\/jats:sub>\n -carrying plasmid in the B2-ST95 worldwide pandemic lineage, characterized by the virulence potential of ExPEC or UPEC but a low prevalence of antimicrobial resistance, would raise public health concerns.\n <\/jats:p>\n <\/jats:sec>\n \n IMPORTANCE<\/jats:title>\n \n ESBLs are plasmid-mediated enzymes that confer resistance to clinically significant antimicrobial agents, such as broad-spectrum cephalosporins. Recently, the rapid spread of CTX-M-type ESBL-producing\n E. coli<\/jats:italic>\n has become a global issue, including in Japan, where ESBL production in human pathogenic\n E. coli<\/jats:italic>\n , such as the ExPEC and UPEC lineages, which typically harbor several virulence genes, is a severe public health concern. To date, VEB (Vietnamese extended-spectrum \u03b2-lactamase) producers have been found only in hospital wastewater and rivers in Japan. Thus, we describe the first detection of a very rare human-derived blaVEB-1 gene in the\n E. coli<\/jats:italic>\n B2-ST95 pandemic clonal lineage that is highly associated with ExPEC and UPEC in a Japanese clinical setting. Furthermore, we characterized the genomic features of plasmids harboring the class 1 integron-borne blaVEB-1. Our findings highlight the significance of closely monitoring ESBL-producing\n E. coli<\/jats:italic>\n isolates to prevent the potential dissemination of this resistance determinant in Japan.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1128\/spectrum.00523-24","type":"journal-article","created":{"date-parts":[[2024,9,17]],"date-time":"2024-09-17T17:05:29Z","timestamp":1726592729000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["First detection of VEB-1 extended-spectrum \u03b2-lactamase-producing\n Escherichia coli<\/i>\n clinical isolate in Japan"],"prefix":"10.1128","volume":"12","author":[{"given":"Junko","family":"Shindou","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Tokyo Metropolitan Tama Medical Center, Tokyo, 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