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DPB is characterized by diffuse pulmonary lesions, inflammation of the respiratory bronchioles, and bacterial infections of the airway. Historically, sputum cultures reveal\n              <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n              in 22% of DPB patients, increasing to 60% after 4 years from disease onset. Although DPB patients have a known susceptibility to respiratory\n              <jats:italic>P. aeruginosa<\/jats:italic>\n              infections, as is observed in other chronic lung diseases such as cystic fibrosis (CF), the characterization of DPB\n              <jats:italic>P. aeruginosa<\/jats:italic>\n              strains is limited. In this study, we characterized 24 strains obtained from a cohort of DPB patients for traits previously associated with virulence, including growth, motility, antibiotic susceptibility, lipopolysaccharide structure, and genomic diversity. Our cohort of DPB\n              <jats:italic>P. aeruginosa<\/jats:italic>\n              strains exhibits considerable genomic variability when compared with isolates from people with cystic fibrosis chronically colonized with\n              <jats:italic>P. aeruginosa<\/jats:italic>\n              and acute\n              <jats:italic>P. aeruginosa<\/jats:italic>\n              infection isolates. Similar to CF, DPB\n              <jats:italic>P. aeruginosa<\/jats:italic>\n              strains produce a diverse array of modified lipid A structures. Antibiotic susceptibility testing revealed increased resistance to erythromycin, a representative agent of the macrolide antibiotics used to manage DPB patients. Differences in the O-antigen type among\n              <jats:italic>P. aeruginosa<\/jats:italic>\n              strains collected from these different backgrounds were also observed. Ultimately, the characterization of DPB\n              <jats:italic>P. aeruginosa<\/jats:italic>\n              strains highlights several unique qualities of\n              <jats:italic>P. aeruginosa<\/jats:italic>\n              strains collected from chronically diseased airways, underscoring the challenges in treating DPB, CF, and other obstructive respiratory disease patients with\n              <jats:italic>P. aeruginosa<\/jats:italic>\n              infections.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Diffuse panbronchiolitis (DPB), a chronic lung disease characterized by persistent\n              <jats:italic>P. aeruginosa<\/jats:italic>\n              infection, serves as an informative comparator to more common chronic lung diseases, such as cystic fibrosis (CF). This study aimed to better address the interplay between\n              <jats:italic>P. aeruginosa<\/jats:italic>\n              and chronically compromised airway environments through the examination of DPB\n              <jats:italic>P. aeruginosa<\/jats:italic>\n              strains, as existing literature regarding DPB is limited to case reports, case series, and clinical treatment guidelines. The evaluation of these features in the context of DPB, in tandem with prevailing knowledge of\n              <jats:italic>P. aeruginosa<\/jats:italic>\n              strains collected from more common chronic lung diseases (e.g., CF), can aid in the development of more effective strategies to combat respiratory\n              <jats:italic>P. aeruginosa<\/jats:italic>\n              infections in patients with chronic lung diseases.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.00530-24","type":"journal-article","created":{"date-parts":[[2024,10,8]],"date-time":"2024-10-08T15:46:54Z","timestamp":1728402414000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Characterization of\n            <i>Pseudomonas aeruginosa<\/i>\n            from subjects with diffuse panbronchiolitis"],"prefix":"10.1128","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-4209-4723","authenticated-orcid":false,"given":"Charles M.","family":"Met","sequence":"first","affiliation":[{"name":"Department of Microbial Pathogenesis, University of Maryland \u2013 Baltimore, Baltimore, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2798-1382","authenticated-orcid":false,"given":"Casey E.","family":"Hofstaedter","sequence":"additional","affiliation":[{"name":"Department of Microbial Pathogenesis, University of Maryland \u2013 Baltimore, Baltimore, Maryland, USA"},{"name":"Medical Scientist Training Program, University of Maryland \u2013 Baltimore, Baltimore, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1151-3242","authenticated-orcid":false,"given":"Ian P.","family":"O'Keefe","sequence":"additional","affiliation":[{"name":"Department of 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USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4918-6179","authenticated-orcid":false,"given":"Matthew E.","family":"Sherman","sequence":"additional","affiliation":[{"name":"Department of Microbial Pathogenesis, University of Maryland \u2013 Baltimore, Baltimore, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9620-2525","authenticated-orcid":true,"given":"Yohei","family":"Doi","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7337-7154","authenticated-orcid":true,"given":"David A.","family":"Rasko","sequence":"additional","affiliation":[{"name":"Department of Microbial Pathogenesis, University of Maryland \u2013 Baltimore, Baltimore, Maryland, USA"},{"name":"Department of 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