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We have previously shown that the antigenomic PE2 (agPE2) in many viruses in the\n                      <jats:italic>Rubulavirinae<\/jats:italic>\n                      subfamily is located within the coding region of the viral RNA polymerase L gene. Sosuga virus (SOSV), belonging to the\n                      <jats:italic>Rubulavirinae<\/jats:italic>\n                      subfamily, is highly pathogenic to humans, thus necessitating high-level containment facilities for infectious virus research. The use of a minigenome system permits studies of viral RNA synthesis at lower biosafety levels. Because minigenomes of negative-strand RNA viruses generally comprise only the untranslated regions, agPE2 within the L coding region\u2014such as those found in\n                      <jats:italic>Rubulavirinae<\/jats:italic>\n                      like SOSV\u2014is typically omitted. However, generating an SOSV minigenome that retains agPE2 led to a pronounced increase in activity, enabling a detailed examination of the role of agPE2 in SOSV replication. In many\n                      <jats:italic>Rubulavirinae<\/jats:italic>\n                      , the agPE2 not only acts as a promoter but also encodes part of the L protein, resulting in a distinct motif at the C-terminus of the L protein. We have further shown that this motif is preserved even in\n                      <jats:italic>Rubulavirinae<\/jats:italic>\n                      that no longer contain the agPE2 within the L gene.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Paramyxoviruses are classified into three major subfamilies:\n                        <jats:italic>Orthoparamyxovirinae<\/jats:italic>\n                        ,\n                        <jats:italic>Avulavirinae<\/jats:italic>\n                        , and\n                        <jats:italic>Rubulavirinae<\/jats:italic>\n                        . All paramyxovirus genomes and antigenomes possess bipartite promoters, comprising two elements: promoter element 1 (PE1) at the 3\u2032 end and promoter element 2 (PE2) located internally. We previously revealed that, in many\n                        <jats:italic>Rubulavirinae<\/jats:italic>\n                        , the antigenomic PE2 lies within the coding region of the viral RNA polymerase L gene. In this study, we used Sosuga virus, a member of the\n                        <jats:italic>Rubulavirinae<\/jats:italic>\n                        subfamily, to elucidate the role of antigenomic PE2 in viral replication. Because the PE2 region encodes part of the L protein, its presence leads to a distinctive motif at the C-terminus of L protein. Notably, this motif is conserved in all\n                        <jats:italic>Rubulavirinae<\/jats:italic>\n                        , including those that do not harbor the antigenomic PE2 within their L gene, indicating its importance in viral propagation.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.00534-25","type":"journal-article","created":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T09:00:25Z","timestamp":1744275625000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Functional analysis of promoter element 2 within the viral polymerase gene of an emerging paramyxovirus, Sosuga virus"],"prefix":"10.1128","volume":"13","author":[{"given":"Lipi","family":"Akter","sequence":"first","affiliation":[{"name":"Transboundary Animal Diseases Research Center, Joint Faculty of Veterinary Medicine, Kagoshima 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