{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:52:31Z","timestamp":1786117951224,"version":"build-2736575974"},"reference-count":57,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["RTG 2501"],"award-info":[{"award-number":["RTG 2501"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,8,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Three novel\n                      <jats:italic toggle=\"yes\">Klebsiella<\/jats:italic>\n                      phages, PMBT12, PMBT63, and PMBT64, were isolated and characterized. The phages revealed distinct morphologies, phylogenetic backgrounds, and a high degree of genomic divergence. PMBT12 and PMBT64 exhibited narrow host ranges, whereas PMBT63 demonstrated a broader host range across the tested\n                      <jats:italic toggle=\"yes\">Klebsiella<\/jats:italic>\n                      strains with diverse sequence and capsule types. PMBT63 possesses a large genome that approaches the size of jumbo T4-like phages, encodes 16 tRNAs, and exhibits high thermal stability up to 70\u00b0C. A conserved Hoc-like protein in PMBT63 was identified and structurally characterized, showing a highly conserved genomic context across diverse\n                      <jats:italic toggle=\"yes\">Enterobacteriaceae<\/jats:italic>\n                      phages. Computational analyses predicted putative mucin-binding sites within this protein, which was corroborated experimentally by enhanced phage retention on mucin-coated surfaces.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      These findings identify\n                      <jats:italic toggle=\"yes\">Klebsiella<\/jats:italic>\n                      phage PMBT63 as a promising candidate for therapeutic applications. It also highlights the importance of characterizing phages for potential phage-mucin interactions, which may influence their persistence and function within the gastrointestinal tract.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.00536-26","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T13:01:12Z","timestamp":1782738072000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Characterization of\n                    <i>Klebsiella<\/i>\n                    phages PMBT12, PMBT63, and PMBT64 with functional insights into a Hoc-like, mucin-interacting protein of phage PMBT63"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-0313-7792","authenticated-orcid":true,"given":"Anna","family":"Ewertz","sequence":"first","affiliation":[{"name":"Institute of Nutritional and Food Microbiology, Max Rubner-Institut","place":["Kiel, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1383-0970","authenticated-orcid":true,"given":"Erik","family":"Brinks","sequence":"additional","affiliation":[{"name":"Institute of Nutritional and Food Microbiology, Max Rubner-Institut","place":["Kiel, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3639-6663","authenticated-orcid":true,"given":"Gyu-Sung","family":"Cho","sequence":"additional","affiliation":[{"name":"Institute of Nutritional and Food Microbiology, Max Rubner-Institut","place":["Kiel, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6244-7947","authenticated-orcid":true,"given":"Frank","family":"Hille","sequence":"additional","affiliation":[{"name":"Institute of Nutritional and Food Microbiology, Max Rubner-Institut","place":["Kiel, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9726-1845","authenticated-orcid":true,"given":"Charles M. A. P.","family":"Franz","sequence":"additional","affiliation":[{"name":"Institute of Nutritional and Food Microbiology, Max Rubner-Institut","place":["Kiel, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.07.003"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1501049112"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00078-15"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2022.103998"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00001-19"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-38295-8"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.4292\/wjgpt.v8.i3.162"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics9030135"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2019.01.014"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00066-18"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciy947"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms13333"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2022.101208"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsestwater.3c00844"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1305923110"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-48560-2"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.3390\/v14081598"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btac776"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","unstructured":"Bouras G Grigson SR Mirdita M Heinzinger M Papudeshi B Mallawaarachchi V Green R Kim RS Mihalia V Psaltis AJ Wormald P-J Vreugde S Steinegger M Edwards RA. 2025. Protein structure informed bacteriophage genome annotation with Phold. bioRxiv. doi:10.1101\/2025.08.05.668817","DOI":"10.1101\/2025.08.05.668817"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbac487"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioadv\/vbad101"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-24448-3"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad382"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-022-01488-1"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.3390\/v12111268"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btx157"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.3390\/v8030066"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btab007"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-49516-2"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab688"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2116197119"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.01554"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2018.09.004"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2019.00784"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2023.112048"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2019.00513"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.13.47"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2018.08.006"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciad514"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S330560"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-22239-3"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.3390\/v13010063"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2007-4"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2011.06.009"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-7-355"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2508(03)00069-X"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.27849-0"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.3.649-659.2001"},{"key":"e_1_3_4_50_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.01876"},{"key":"e_1_3_4_51_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00574"},{"key":"e_1_3_4_52_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2024.1455710"},{"key":"e_1_3_4_53_2","doi-asserted-by":"publisher","DOI":"10.1089\/phage.2022.0021"},{"key":"e_1_3_4_54_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07219.x"},{"key":"e_1_3_4_55_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560050923"},{"key":"e_1_3_4_56_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1508355112"},{"key":"e_1_3_4_57_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msp108"},{"key":"e_1_3_4_58_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-025-06322-5"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00536-26","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00536-26","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T14:43:16Z","timestamp":1786027396000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.00536-26"}},"subtitle":[],"editor":[{"given":"Mark William","family":"Pandori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,8,4]]},"references-count":57,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8,4]]}},"alternative-id":["10.1128\/spectrum.00536-26"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.00536-26","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,4]]},"assertion":[{"value":"2026-02-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00536-26"}}