{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T22:08:30Z","timestamp":1780956510346,"version":"3.54.1"},"reference-count":81,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2021,9,3]],"date-time":"2021-09-03T00:00:00Z","timestamp":1630627200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,9,3]],"date-time":"2021-09-03T00:00:00Z","timestamp":1630627200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01AI110708"],"award-info":[{"award-number":["R01AI110708"]}],"id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DGE-1762114"],"award-info":[{"award-number":["DGE-1762114"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2021,9,3]]},"abstract":"<jats:p>\n                    <jats:named-content content-type=\"genus-species\">Giardia lamblia<\/jats:named-content>\n                    is an intestinal parasite that colonizes the small intestine and causes diarrhea, which can lead to dehydration and malnutrition.\n                    <jats:italic>Giardia<\/jats:italic>\n                    actin (\n                    <jats:italic>Gl<\/jats:italic>\n                    Actin) has a conserved role in\n                    <jats:italic>Giardia<\/jats:italic>\n                    cells, despite being a highly divergent protein with none of the conserved regulators found in model organisms. Here, we identify and localize 46 interactors of polymerized actin.\n                  <\/jats:p>","DOI":"10.1128\/spectrum.00558-21","type":"journal-article","created":{"date-parts":[[2021,7,21]],"date-time":"2021-07-21T10:30:17Z","timestamp":1626863417000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Identification of Actin Filament-Associated Proteins in Giardia lamblia"],"prefix":"10.1128","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8143-1352","authenticated-orcid":true,"given":"Melissa C.","family":"Steele-Ogus","sequence":"first","affiliation":[{"name":"Department of Biology, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard S.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Genome Sciences, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"MacCoss","sequence":"additional","affiliation":[{"name":"Department of Genome Sciences, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9298-3264","authenticated-orcid":true,"given":"Alexander R.","family":"Paredez","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a018226"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature07685"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1143837"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.105.070052"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00173163"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1018593108"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1705096114"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2012.11.005"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2006.02.015"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00041-14"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-538-5_9"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2557"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2009.01367.x"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.3.1922-1923.2001"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00248-17"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.22074\/cellj.2015.507"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004571"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw1105"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn631"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.5.4.753-761.2006"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1106904108"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.5776"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2014.07.038"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.215897"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13630-016-0042-4"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1017\/S1431927613013275"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2015.04.017"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/bs.apar.2019.11.001"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0500349102"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-017-0361-y"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2017.10.007"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01003-16"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12054"},{"key":"e_1_3_3_35_2","doi-asserted-by":"crossref","unstructured":"Hardin WR M Alas GC Taparia N Thomas EB Hvorecny KL Halpern AR T\u016fmov\u00e1 P Vaughan JC Sniadecki NJ Paredez AR. 2021. The Giardia lamellipodium-like ventrolateral flange supports attachment and rapid cytokinesis. BioRxiv. doi:10.1101\/2021.01.31.429041.","DOI":"10.1101\/2021.01.31.429041"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.2004.tb00165.x"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0043783"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.3109\/10409238.2010.488217"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.595917"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a040290"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2004.00210.x"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1515\/hsz-2016-0171"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2020.111714"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81633-5"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1080\/19491034.2016.1267093"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2020.11.009"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-062917-012644"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2016.12.006"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2016.08.011"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005756"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-021-07062-6"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20100096"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(02)00422-5"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.095711"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.12.10.2907"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a017004"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2008.00777.x"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1855"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.00675"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2013.06.015"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.103440"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182020002292"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.114.6.1061"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41594-020-00530-0"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.12.007"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.137.3.649"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.04.087"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.5772\/intechopen.70243"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(82)90100-X"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0020-7519(02)00228-x"},{"key":"e_1_3_3_71_2","doi-asserted-by":"crossref","unstructured":"Nosala C Dawson SC. 2017. The ventral disc is a flexible microtubule organelle that depends on domed ultrastructure for functional attachment of Giardia lamblia . bioRxiv. doi:10.1101\/213421.","DOI":"10.1101\/213421"},{"key":"e_1_3_3_72_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-019-3478-8"},{"key":"e_1_3_3_73_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001442"},{"key":"e_1_3_3_74_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.03276"},{"key":"e_1_3_3_75_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201200439"},{"key":"e_1_3_3_76_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac025747h"},{"key":"e_1_3_3_77_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac0341261"},{"key":"e_1_3_3_78_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201000650"},{"key":"e_1_3_3_79_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E19-07-0406"},{"key":"e_1_3_3_80_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.2006.01706.x"},{"key":"e_1_3_3_81_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz984"},{"key":"e_1_3_3_82_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1106"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/Spectrum.00558-21","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/Spectrum.00558-21","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,8]],"date-time":"2022-03-08T03:42:28Z","timestamp":1646710948000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/Spectrum.00558-21"}},"subtitle":[],"editor":[{"given":"Christina A.","family":"Cuomo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,9,3]]},"references-count":81,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,9,3]]}},"alternative-id":["10.1128\/Spectrum.00558-21"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.00558-21","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.05.19.444898","asserted-by":"object"}]},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,3]]},"assertion":[{"value":"2021-06-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-07-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00558-21"}}