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To better understand whether this bacterium could be used for malaria control, we quantified\n              <jats:italic>Asaia<\/jats:italic>\n              in\n              <jats:italic>An.\u2009gambiae s.l<\/jats:italic>\n              populations in malaria endemic regions examining co-infection with\n              <jats:italic>Plasmodium falciparum<\/jats:italic>\n              . Adult\n              <jats:italic>Anopheles<\/jats:italic>\n              mosquitoes were collected across two different eco-geographical localities in Cameroon, during both the dry and wet seasons. DNA was extracted from whole individual mosquitoes, and real time-qPCR amplification of the\n              <jats:italic>16S ribosomal<\/jats:italic>\n              RNA was used to quantify\n              <jats:italic>Asaia<\/jats:italic>\n              in both\n              <jats:italic>An. gambiae<\/jats:italic>\n              and\n              <jats:italic>An. coluzzii<\/jats:italic>\n              samples. We also detected and quantified\n              <jats:italic>P. falciparum<\/jats:italic>\n              infection in the same mosquitoes. The density of\n              <jats:italic>Asaia<\/jats:italic>\n              was successfully quantified in a total of 864 field mosquitoes, comprising of 439\n              <jats:italic>An. gambiae<\/jats:italic>\n              from Bankeng and 424\n              <jats:italic>An. coluzii<\/jats:italic>\n              collected from Gounougou. Interestingly, a higher prevalence of\n              <jats:italic>Asaia<\/jats:italic>\n              in\n              <jats:italic>An. gambiae<\/jats:italic>\n              (88.3%) compared to\n              <jats:italic>An. coluzzii<\/jats:italic>\n              (80.9%) was observed. Moreover, the density of\n              <jats:italic>Asaia<\/jats:italic>\n              in both species was significantly affected by seasonal changes in the two localities. Furthermore, a significant difference between the infection densities of\n              <jats:italic>Asaia<\/jats:italic>\n              and the\n              <jats:italic>Plasmodium<\/jats:italic>\n              infection status in the two species was recorded. However, no correlation was observed between the number of\n              <jats:italic>Asaia<\/jats:italic>\n              and\n              <jats:italic>P. falciparum<\/jats:italic>\n              infections. This study provides evidence that naturally occurring\n              <jats:italic>Asaia<\/jats:italic>\n              infection is not correlated to\n              <jats:italic>P. falciparum<\/jats:italic>\n              development within\n              <jats:italic>An. gambiae<\/jats:italic>\n              and\n              <jats:italic>An. coluzzii<\/jats:italic>\n              . Nevertheless, further studies incorporating experimental infections are required to better investigate the correlation between\n              <jats:italic>Anopheles<\/jats:italic>\n              mosquitoes,\n              <jats:italic>Asaia,<\/jats:italic>\n              and\n              <jats:italic>Plasmodium<\/jats:italic>\n              .\n            <\/jats:p>\n            <jats:sec>\n              <jats:title>IMPORTANCE<\/jats:title>\n              <jats:p>\n                The symbiont\n                <jats:italic>Asaia<\/jats:italic>\n                has emerged as a promising candidate for paratransgenic control of malaria, but further analysis of its biology and genetics across Africa is necessary. In this study, we investigated and quantified the influence of\n                <jats:italic>Asaia<\/jats:italic>\n                in naturally infected\n                <jats:italic>An. gambiae s.l<\/jats:italic>\n                . populations with the malaria parasite\n                <jats:italic>Plasmodium falciparum<\/jats:italic>\n                . Genomic DNA was extracted from whole individual mosquitoes collected from two localities, and\n                <jats:italic>Asaia<\/jats:italic>\n                was quantified using real-time qPCR by amplification of the 16S ribosomal RNA gene. We also detected and quantified\n                <jats:italic>Plasmodium falciparum<\/jats:italic>\n                infection in the same mosquitoes and established the correlation between\n                <jats:italic>Asaia<\/jats:italic>\n                and\n                <jats:italic>Plasmodium<\/jats:italic>\n                coinfection. This study provides evidence that naturally occurring\n                <jats:italic>Asaia<\/jats:italic>\n                infection is not correlated with\n                <jats:italic>P. falciparum<\/jats:italic>\n                development within\n                <jats:italic>An. gambiae<\/jats:italic>\n                and\n                <jats:italic>An. coluzzii<\/jats:italic>\n                mosquitoes.\n              <\/jats:p>\n            <\/jats:sec>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.00567-24","type":"journal-article","created":{"date-parts":[[2024,11,12]],"date-time":"2024-11-12T14:00:56Z","timestamp":1731420056000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Contrasting patterns of\n            <i>Asaia<\/i>\n            association with\n            <i>Plasmodium falciparum<\/i>\n            between field-collected\n            <i>Anopheles gambiae<\/i>\n            and\n            <i>Anopheles coluzzii<\/i>\n            from Cameroon"],"prefix":"10.1128","volume":"12","author":[{"given":"Claudine Gr\u00e2ce","family":"Tatsinkou Maffo","sequence":"first","affiliation":[{"name":"Department of Medical Entomology, Centre for Research in Infectious Diseases (CRID), LSTM Research Unit","place":["Yaound\u00e9, Cameroon"]},{"name":"Department of Animal Biology and Physiology, Faculty of Science, University of Yaound\u00e9 1","place":["Yaound\u00e9, Cameroon"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6780-5394","authenticated-orcid":true,"given":"Maurice Marcel","family":"Sandeu","sequence":"additional","affiliation":[{"name":"Department of Medical Entomology, Centre for Research in Infectious Diseases (CRID), LSTM Research Unit","place":["Yaound\u00e9, Cameroon"]},{"name":"Department of Microbiology and Infectious Diseases, School of Veterinary Medicine and Sciences, University of Ngaound\u00e9r\u00e9","place":["Ngaound\u00e9r\u00e9, Cameroon"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micareme","family":"Tchoupo","sequence":"additional","affiliation":[{"name":"Department of Medical Entomology, Centre for Research in Infectious Diseases (CRID), LSTM Research Unit","place":["Yaound\u00e9, Cameroon"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fleuriane Metissa","family":"Dondji Kamga","sequence":"additional","affiliation":[{"name":"Department of Medical Entomology, Centre for Research in Infectious Diseases (CRID), LSTM Research Unit","place":["Yaound\u00e9, Cameroon"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leon M. 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