{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:42:04Z","timestamp":1785436924957,"version":"3.56.0"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"The Hunan Provincial Key Laboratory of Anti-ResistanceMicrobial Drugs, the Third Hospital of Changsha","award":["2023TP1013"],"award-info":[{"award-number":["2023TP1013"]}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"crossref","award":["2023JJ30301"],"award-info":[{"award-number":["2023JJ30301"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,10,7]]},"abstract":"ABSTRACT<\/jats:title>\n \n \n \n Multi-drug-resistant\n Aeromonas hydrophila<\/jats:italic>\n infections are becoming increasingly threatening, and the development of novel antimicrobial drugs is indispensable. Herein, we demonstrate that this novel peptide is highly active against colistin-resistant\n A. hydrophila<\/jats:italic>\n strain and shows sustained killing efficacy\n in vivo<\/jats:italic>\n . Mechanistic studies showed that D-Q7 interacted with phosphatidylglycerol and lipopolysaccharide in the bacterial cell membrane, with an increase in intracellular ROS as well as a decrease in ATP level, ultimately leading to cell membrane disruption and bacterial death. Importantly, our study identified gene3832 as a potential regulator of membrane permeability, which may act as a potential modulator of bacterial susceptibility to D-Q7. The role of gene3832 was further confirmed by gene knockout and complementation assays. Consistently, we observed that gene3832 was also involved in biofilm formation in the colistin-resistant\n A. hydrophila<\/jats:italic>\n strain. Collectively, our study provides an effective antimicrobial strategy with potential targets for the treatment of drug-resistant\n A. hydrophila<\/jats:italic>\n infection.\n <\/jats:p>\n \n IMPORTANCE<\/jats:title>\n \n As an environmental, zoonotic pathogen,\n Aeromonas hydrophila<\/jats:italic>\n remains a major pathogenic bacterium, bringing large economic losses and eco-environmental pressure during the event of large-scale infection. Currently, the occurrence of colistin-resistant\n A. hydrophila<\/jats:italic>\n poses a threat to public health owing to the lack of effective prevention and therapeutics. D-Q7 is a D-type antimicrobial peptide (AMP) with potent sterilization activity against gram-negative ESKAPE pathogens; it is thus of considerable interest to evaluate whether D-Q7 represents a promising therapeutic candidate against this pathogen. Consequently, we found that D-Q7 was a potent antibacterial agent that killed colistin-resistant\n A. hydrophila 23-c-23 in vitro<\/jats:italic>\n and in a mouse epicutaneous model of\n 23-c-23<\/jats:italic>\n infection. In addition, we found that\n gene3832<\/jats:italic>\n , as a potential transmembrane autotransporter, is related to bacterial resistance to D-Q7. Importantly, our study here will help guide the future design and optimization of novel AMPs to combat colistin-resistant\n A. hydrophila<\/jats:italic>\n .\n <\/jats:p>\n <\/jats:sec>\n <\/jats:sec>","DOI":"10.1128\/spectrum.00590-25","type":"journal-article","created":{"date-parts":[[2025,8,29]],"date-time":"2025-08-29T13:00:32Z","timestamp":1756472432000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Killing of colistin-resistant\n Aeromonas hydrophila<\/i>\n by a synthetic peptide"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0358-1421","authenticated-orcid":true,"given":"Leisheng","family":"Sun","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/053w1zy07","id-type":"ROR","asserted-by":"publisher"}],"name":"Key Laboratory of Study and Discovery of Small Targeted Molecules of Hunan Province, Department of Pharmacy, School of Medicine, Hunan Normal University","place":["Changsha, PR China"]},{"id":[{"id":"https:\/\/ror.org\/04w3qme09","id-type":"ROR","asserted-by":"publisher"}],"name":"Hunan Provincial Key Laboratory of Anti-Resistance Microbial Drugs, The Third Hospital of Changsha","place":["Changsha, PR China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zonghan","family":"Jiang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01dzed356","id-type":"ROR","asserted-by":"publisher"}],"name":"Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University","place":["Changsha, PR China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dingding","family":"Li","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/053w1zy07","id-type":"ROR","asserted-by":"publisher"}],"name":"Key Laboratory of Study and Discovery of Small Targeted Molecules of Hunan Province, Department of Pharmacy, School of Medicine, Hunan Normal University","place":["Changsha, PR China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Tian","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01fj5gf64","id-type":"ROR","asserted-by":"publisher"}],"name":"Hunan Institute of Animal and Veterinary Science, Hunan Academy of Agricultural Sciences","place":["Changsha, PR China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junqi","family":"Liu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01fj5gf64","id-type":"ROR","asserted-by":"publisher"}],"name":"Hunan Institute of Animal and Veterinary Science, Hunan Academy of Agricultural Sciences","place":["Changsha, PR China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6612-6541","authenticated-orcid":true,"given":"Zhiliang","family":"Sun","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01dzed356","id-type":"ROR","asserted-by":"publisher"}],"name":"Hunan Engineering Research Center of Livestock and Poultry Health Care, College of Veterinary Medicine, Hunan Agricultural University","place":["Changsha, PR China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiph.2015.10.006"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2015.1058479"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eimce.2023.02.004"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2024.178247"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222111691"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27092675"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.2174\/0929866526666190822165812"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.4155\/fmc-2021-0134"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1918427117"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsinfecdis.2c00640"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.13020"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00071-21"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2017.26"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2024.116274"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.1032969"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1080\/07391102.2024.2304683"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2008.11.005"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/ecosalplus.ESP-0001-2019"}],"container-title":["Microbiology Spectrum"],"original-title":[],"contributor":[{"given":"Rui","family":"Bao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"reviewer"}]},{"given":"Sattar","family":"Taheri-Araghi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"reviewer"}]}],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00590-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00590-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T13:10:51Z","timestamp":1759842651000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.00590-25"}},"subtitle":[],"editor":[{"given":"Jinshui","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,10,7]]},"references-count":18,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,10,7]]}},"alternative-id":["10.1128\/spectrum.00590-25"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.00590-25","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,7]]},"assertion":[{"value":"2025-02-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00590-25"}}