{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T12:40:32Z","timestamp":1785415232151,"version":"3.56.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,11,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Burkholderia pseudomallei (Bp<\/jats:italic>\n                      ) is a gram-negative bacterium found in soil and surface water. It is also the pathogen that causes melioidosis disease in humans and animals. This study aimed to obtain the whole genome sequence of the endemic strain of\n                      <jats:italic toggle=\"yes\">Bp<\/jats:italic>\n                      in Hainan, using third-generation sequencing (TGS) technology, and elucidate the genome structure, function, and genetic evolution. Additionally, the study aimed to achieve rapid and specific identification of these endemic strains using specific high-sensitivity enzymatic reporter unlocking (SHERLOCK) detection technology, providing a new strategy for the early diagnosis of melioidosis. Utilizing the PacBio platform for TGS technology, we completed whole genome sequencing of 16\n                      <jats:italic toggle=\"yes\">Bp<\/jats:italic>\n                      strains from Hainan. High-precision and complete genome sequences were obtained through quality control and genome assembly of the sequencing data. Additionally, we established a nucleic acid detection technology platform based on SHERLOCK, which could be completed from nucleic acid extraction to result reading within 1\u22122 hours, demonstrating good sensitivity and specificity (both are 100%). The lateral chromatography strip method does not require special equipment and holds promise as an immediate screening method for the early diagnosis of melioidosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      Melioidosis is a highly pathogenic infectious disease caused by a gram-negative bacterium of\n                      <jats:italic toggle=\"yes\">Burkholderia pseudomallei<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">Bp<\/jats:italic>\n                      ). The traditional gold standard for diagnosing melioidosis is still isolation and culture from clinical samples. Although this method has high specificity, it has low sensitivity and is time-consuming, which often leads to misdiagnosis or missed diagnosis of melioidosis, affecting subsequent treatment. In this study, recombinase polymerase amplification technology and clustered regularly interspaced short palindromic repeats\/Cas13a technology were combined to establish the Specific High-sensitivity Enzymatic Reporter Unlocking detection technology, which can achieve rapid and accurate identification of\n                      <jats:italic toggle=\"yes\">Bp<\/jats:italic>\n                      , providing a new method for the early diagnosis of melioidosis.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.00592-25","type":"journal-article","created":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T13:00:18Z","timestamp":1758546018000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Whole genome study and construction of SHERLOCK detection method for endemic strains of\n                    <i>Burkholderia pseudomallei<\/i>\n                    in Hainan based on third-generation sequencing"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3341-7588","authenticated-orcid":false,"given":"Junjie","family":"Hu","sequence":"first","affiliation":[{"name":"Department of clinical Laboratory, Affiliated Cancer Hospital of Hainan Medical University, Hainan Cancer Hospital","place":["Haikou, People's Republic of China"]},{"id":[{"id":"https:\/\/ror.org\/004eeze55","id-type":"ROR","asserted-by":"publisher"}],"name":"The First Clinical School of Hainan Medical University","place":["Haihou, People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanshan","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of clinical Laboratory, Affiliated Cancer Hospital of Hainan Medical University, Hainan Cancer Hospital","place":["Haikou, People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeng","family":"Zeng","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03p31hk68","id-type":"ROR","asserted-by":"publisher"}],"name":"Sanya Hospital of Traditional Chinese Medicine","place":["Sanya, People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of clinical Laboratory, Affiliated Cancer Hospital of Hainan Medical University, Hainan Cancer Hospital","place":["Haikou, People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weihua","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of clinical Laboratory, Affiliated Cancer Hospital of Hainan Medical University, Hainan Cancer Hospital","place":["Haikou, People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhichao","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of clinical Laboratory, Affiliated Cancer Hospital of Hainan Medical University, Hainan Cancer Hospital","place":["Haikou, People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengmiao","family":"Fu","sequence":"additional","affiliation":[{"name":"Hainan Lvtou Medical Laboratory Center","place":["Haikou, People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linhai","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, The Affiliated Qingyuan Hospital (Qingyuan People\u2019s Hospital), Guangzhou Medical University","place":["Qingyuan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8822-2542","authenticated-orcid":false,"given":"Bin","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, The Affiliated Qingyuan Hospital (Qingyuan People\u2019s Hospital), Guangzhou Medical University","place":["Qingyuan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1193-2890","authenticated-orcid":false,"given":"Xinping","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of clinical Laboratory, Affiliated Cancer Hospital of Hainan Medical University, Hainan Cancer Hospital","place":["Haikou, People's Republic of China"]},{"id":[{"id":"https:\/\/ror.org\/004eeze55","id-type":"ROR","asserted-by":"publisher"}],"name":"The First Clinical School of Hainan Medical University","place":["Haihou, People's Republic of China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2016.263"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2016.07.003"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2712.211756"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2015.8"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000000869"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-019-4627-6"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2024.105654"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13104-024-06717-8"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0403302101"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000659"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.01259-20"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0261382"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01448"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000527"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaq0180"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aam9321"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aas8836"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2017.04.008"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.3466"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.3389\/fpubh.2023.1153352"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0010659"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e3181bf542c"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.7.3312-3316.2003"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.107"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268818002741"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2022.2139063"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2022.105637"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01936-24"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-28843-7"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.980525"}],"container-title":["Microbiology Spectrum"],"original-title":[],"contributor":[{"given":"Narisara","family":"Chantratita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"reviewer"}]}],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00592-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00592-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T14:06:45Z","timestamp":1762265205000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.00592-25"}},"subtitle":[],"editor":[{"given":"Vittal Prakash","family":"Ponraj","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,11,4]]},"references-count":30,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2025,11,4]]}},"alternative-id":["10.1128\/spectrum.00592-25"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.00592-25","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,4]]},"assertion":[{"value":"2025-02-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-31","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00592-25"}}