{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T21:12:56Z","timestamp":1787778776632,"version":"build-2784847793"},"reference-count":13,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T00:00:00Z","timestamp":1733356800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T00:00:00Z","timestamp":1733356800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,12,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Rapid identification of methicillin-susceptible\n              <jats:italic>Staphylococcus aureus<\/jats:italic>\n              (MSSA) bacteremia may optimize antibiotic use and clinical outcomes. The study objective was to assess the impact of the BioFire\u00ae blood culture identification (BCID) polymerase chain reaction (PCR) panel on antibiotic use and clinical outcomes in patients with MSSA bacteremia. This was a retrospective chart review of adult inpatients with MSSA bacteremia during the pre-PCR (June 2018\u2013December 2019) and post-PCR (June 2020\u2013December 2021) implementation periods. The primary endpoint was the difference between the pre-PCR and post-PCR groups in time to optimal MSSA antibiotic therapy (oxacillin or cefazolin). Secondary endpoints included duration of anti-MRSA antibiotic use, in-hospital mortality, hospital and ICU lengths of stay, duration of bacteremia, and 30-day MSSA-related and all-cause readmissions. Two hundred patients were included in the study (100 per group). Median time to optimal therapy was reduced by 19.9 hours in the post-PCR group (49 vs 29.1 hours, respectively,\n              <jats:italic>P<\/jats:italic>\n              &lt; 0.001). PCR implementation was associated with significant reductions in the initiation of empiric anti-MRSA antibiotic therapy (98% vs 84%,\n              <jats:italic>P<\/jats:italic>\n              = 0.001), duration of empiric anti-MRSA antibiotic use (44.2 vs 20.9 hours,\n              <jats:italic>P<\/jats:italic>\n              &lt; 0.001), and duration of bacteremia (68.6 vs 47.3 hours,\n              <jats:italic>P<\/jats:italic>\n              = 0.015). No significant differences in length of stay, in-hospital mortality, or 30-day MSSA-related or all-cause readmissions were observed. In patients with MSSA bacteremia, BCID PCR panel implementation decreased time to optimal MSSA antibiotic therapy, duration of bacteremia, and duration of empiric anti-MRSA antibiotic therapy.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              In this retrospective study of 200 patients with methicillin-susceptible\n              <jats:italic>Staphylococcus aureus<\/jats:italic>\n              (MSSA) bacteremia, the implementation of the BioFire\u00ae blood culture identification polymerase chain reaction (PCR) panel was associated with a decreased time to optimal MSSA antibiotic therapy and shorter durations of empiric anti-MRSA antibiotic therapy and bacteremia. The findings demonstrate the significant role of rapid PCR testing and routine stewardship review in optimizing antimicrobial therapy and management of MSSA bacteremia.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.00629-24","type":"journal-article","created":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T13:05:53Z","timestamp":1729602353000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Impact of rapid blood culture identification PCR panel on optimal antibiotic use in methicillin-susceptible\n            <i>Staphylococcus aureus<\/i>\n            bacteremia"],"prefix":"10.1128","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-0103-8979","authenticated-orcid":true,"given":"Jahnavi","family":"Yetukuri","sequence":"first","affiliation":[{"name":"Ernest Mario School of Pharmacy, Rutgers University","place":["Piscataway, USA"]},{"name":"Department of Pharmacy, Morristown Medical Center","place":["Morristown, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0629-9950","authenticated-orcid":false,"given":"Dimple","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Morristown Medical Center","place":["Morristown, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5025-6528","authenticated-orcid":false,"given":"Aiman","family":"Bandali","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Overlook Medical Center","place":["Summit, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7027-6987","authenticated-orcid":false,"given":"Pamela","family":"Giordano","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Morristown Medical Center","place":["Morristown, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Roland","sequence":"additional","affiliation":[{"name":"Department of Medicine, Overlook Medical Center","place":["Summit, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason","family":"Kessler","sequence":"additional","affiliation":[{"name":"Department of Medicine, Morristown Medical Center","place":["Morristown, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.06169-11"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofz270"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ308"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofy089"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw118"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/jpids\/piw047"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ447"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2015.12.007"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1086\/656623"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciq146"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2017.07.003"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciz257"},{"key":"e_1_3_3_14_2","first-page":"65","article-title":"Incidence and risk factors for persistent Staphylococcus aureus bacteremia","volume":"9","author":"Jarrell DH","year":"2014","unstructured":"Jarrell DH, Matthias KR, Nix DE. 2014. Incidence and risk factors for persistent Staphylococcus aureus bacteremia. Trends Microbiol 9:65\u201371.","journal-title":"Trends Microbiol"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00629-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00629-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T14:10:59Z","timestamp":1733407859000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.00629-24"}},"subtitle":[],"editor":[{"given":"Kessendri","family":"Reddy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,12,5]]},"references-count":13,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2024,12,5]]}},"alternative-id":["10.1128\/spectrum.00629-24"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.00629-24","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,5]]},"assertion":[{"value":"2024-03-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00629-24"}}