{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:56:37Z","timestamp":1791294997796,"version":"4.1.0"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32302025"],"award-info":[{"award-number":["32302025"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Project of the Industry and Technology System for Modern Agriculture in Hebei Province","award":["HBCT2023040206"],"award-info":[{"award-number":["HBCT2023040206"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,10,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      The health benefits of dietary soy isoflavones are largely mediated by specific microbial metabolites, such as\n                      <jats:italic toggle=\"yes\">O<\/jats:italic>\n                      -desmethylangolensin (\n                      <jats:italic toggle=\"yes\">O<\/jats:italic>\n                      -DMA). However, the diversity and application potential of\n                      <jats:italic toggle=\"yes\">O<\/jats:italic>\n                      -DMA-producing strains remain poorly explored, primarily due to the limited availability of isolated strains, narrow ecological sources, and a lack of practical applications. In this study, an\n                      <jats:italic toggle=\"yes\">O<\/jats:italic>\n                      -DMA-producing bacterium, designated strain FRJF5, was isolated from Chinese stinky tofu under anaerobic conditions and was identified as\n                      <jats:italic toggle=\"yes\">Clostridium beijerinckii<\/jats:italic>\n                      . The biosynthesized\n                      <jats:italic toggle=\"yes\">O<\/jats:italic>\n                      -DMA exhibited an enantiomeric excess (e.e.) of 78.6%. Based on phylogenetic and average nucleotide identity analyses against 235 public\n                      <jats:italic toggle=\"yes\">C. beijerinckii<\/jats:italic>\n                      genomes, the clustering of FRJF5 with strains from diverse habitats\u2014including industrial fermentation settings, animal feces, and soil\u2014highlights the broad ecological diversity within this species. Functional gene mining and intra-species comparative genomics revealed a unique flavonoid metabolism gene cluster in FRJF5. Using apigenin as a representative flavonoid, we confirmed the successful conversion to 3-(4-hydroxyphenyl)-propionic acid. Moreover, the strain was predicted and verified to possess a substantial butyrate-producing capacity. Genomic screening for virulence or antibiotic resistance genes, combined with phenotypic tests (hemolysis, antibiotic susceptibility, and mouse gavage), revealed a favorable safety profile for strain FRJF5. Finally, intervention experiments in a mouse model of colitis supported its potential in alleviating the disease. Collectively, this study identifies\n                      <jats:italic toggle=\"yes\">C. beijerinckii<\/jats:italic>\n                      FRJF5 as a strain capable of simultaneously producing\n                      <jats:italic toggle=\"yes\">O<\/jats:italic>\n                      -DMA and butyrate, highlighting its potential for future applications in functional foods.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Soy isoflavones require gut bacterial conversion into bioactive metabolites\u2014such as the anti-inflammatory compound\n                        <jats:italic toggle=\"yes\">O<\/jats:italic>\n                        -desmethylangolensin (\n                        <jats:italic toggle=\"yes\">O<\/jats:italic>\n                        -DMA)\u2014to exert health benefits. Yet\n                        <jats:italic toggle=\"yes\">O<\/jats:italic>\n                        -DMA-producing strains remain scarce, largely confined to fecal sources, and poorly characterized. Here, we isolated\n                        <jats:italic toggle=\"yes\">Clostridium beijerinckii<\/jats:italic>\n                        FRJF5 from Chinese stinky tofu, an unexplored ecological niche. This strain not only produces enantiomerically enriched\n                        <jats:italic toggle=\"yes\">O<\/jats:italic>\n                        -DMA but also co-produces butyrate, a metabolite known to strengthen gut barrier function. Genomic mining uncovered a unique flavonoid metabolism gene cluster responsible for this dual activity. Combined with favorable safety profiles, FRJF5 emerges as a strong candidate for functional food applications. This work expands the known diversity of\n                        <jats:italic toggle=\"yes\">O<\/jats:italic>\n                        -DMA producers and bridges traditional fermented foods with next-generation probiotic development.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.00632-26","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T13:03:44Z","timestamp":1789650224000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Isolation, genomic characterization, and safety assessment of an\n                    <i>O<\/i>\n                    -desmethylangolensin-producing\n                    <i>Clostridium beijerinckii<\/i>\n                    strain from Chinese Stinky Tofu"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6041-6460","authenticated-orcid":true,"given":"Xia","family":"Guo","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01p884a79","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Life Sciences, Hebei Agricultural University","place":["Baoding, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sifan","family":"Zhang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01p884a79","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Life Sciences, Hebei Agricultural University","place":["Baoding, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyi","family":"Cui","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01p884a79","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Life Sciences, Hebei Agricultural University","place":["Baoding, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01p884a79","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Life Sciences, Hebei Agricultural University","place":["Baoding, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zixuan","family":"Zhao","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01p884a79","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Life Sciences, Hebei Agricultural University","place":["Baoding, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanmian","family":"Wei","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/009fw8j44","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Food Science and Technology, Hebei Agricultural University","place":["Baoding, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8151-3830","authenticated-orcid":true,"given":"Hao","family":"Li","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01p884a79","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Life Sciences, Hebei Agricultural University","place":["Baoding, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2227-8295","authenticated-orcid":true,"given":"Xiu-Ling","family":"Wang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01p884a79","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Life Sciences, Hebei Agricultural University","place":["Baoding, 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