{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:50:09Z","timestamp":1786117809305,"version":"build-2736575974"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"INTERBEV","award":["SECU-19-42"],"award-info":[{"award-number":["SECU-19-42"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,8,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Antimicrobial resistance (AMR) is an important One Health issue affecting the three human, animal, and environmental sectors. The occurrence of\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      resistant to extended-spectrum cephalosporins (ESC-R) is considered a relevant marker for the estimation of the AMR burden. In the dairy industry, veal calves present the highest proportions of ESC-R compared to adults. Since little is known in France about the AMR dynamics from birth to the fattening farm, our observational study determined the proportion of ESC-R\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      using selective media, and characterized by whole-genome sequencing. Bacterial isolates identified at the farm level and at the sorting center, before being sent to the fattening farms. The proportion of ESC-R-positive calves coming from farms in which waste milk was given to calves (30.3%) was higher than in the ones where it was discarded (13.1%). Overall, 12.5% of the veal calves were ESC-R carriers in their farm of origin, and 6.2% of the additional animals became carriers during their transport to the sorting center. Over the 176 ESC-R\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      isolates sequenced, 55.1% (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 97) presented an extended-spectrum beta-lactamase phenotype conferred by a\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>CTX-M<\/jats:sub>\n                      gene, while 44.9% (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 79) presented an AmpC phenotype due to chromosomal mutations. A few specific clones (ST8095 and ST117) were identified as responsible for AMR dissemination across farms. Our results showed that AMR in dairy calves results from cumulative effects of all risk factors along the whole production chain, including feeding calves with antibiotic residues-containing waste milk, animal grouping, and insufficient biosecurity measures in dairy farms.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      Despite the implementation of national action plans, the presence of\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      resistant to extended-spectrum cephalosporins (ESC-R), mainly due to the presence of extended-spectrum beta-lactamases or AmpCs, is still high in many veal-producing countries. In France, previous studies reported antimicrobial resistance (AMR) data during the fattening period and at the slaughterhouse, but data are still scarce on the AMR status of veal calves on their farm of origin. This study aimed at determining the proportion of ESC-R\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      and characterizing bacterial isolates identified at the farm level and at the sorting center, just before being sent to the fattening farms. This is of utmost importance since mathematical models showed that minimizing AMR prevalence upon arrival in the fattening farm is an important factor to mitigate ESC-R carriage during the fattening process. In addition, we are providing important WGS data from the veal calf sector, which can be compared to already-existing data.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.00659-26","type":"journal-article","created":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T13:01:04Z","timestamp":1782133264000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Extended-spectrum beta-lactamases from French veal calves: WGS-based data will help build targeted AMR mitigation strategies"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1333-5603","authenticated-orcid":true,"given":"Marisa","family":"Haenni","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01rk35k63","id-type":"ROR","asserted-by":"publisher"}],"name":"ANSES \u2013 Universit\u00e9 de Lyon \u2013 Unit\u00e9 Antibior\u00e9sistance et Virulence Bact\u00e9riennes","place":["Lyon, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e9ronique","family":"M\u00e9tayer","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01rk35k63","id-type":"ROR","asserted-by":"publisher"}],"name":"ANSES \u2013 Universit\u00e9 de Lyon \u2013 Unit\u00e9 Antibior\u00e9sistance et Virulence Bact\u00e9riennes","place":["Lyon, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoine","family":"Drapeau","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01rk35k63","id-type":"ROR","asserted-by":"publisher"}],"name":"ANSES \u2013 Universit\u00e9 de Lyon \u2013 Unit\u00e9 Antibior\u00e9sistance et Virulence Bact\u00e9riennes","place":["Lyon, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"le Go\u00efc","sequence":"additional","affiliation":[{"name":"Clinique AmiVet","place":["Janz\u00e9, France"]},{"name":"Clinique Vitelliance, R\u00e9seau Cristal","place":["La Guerche de Bretagne, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Yves","family":"Madec","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01rk35k63","id-type":"ROR","asserted-by":"publisher"}],"name":"ANSES \u2013 Universit\u00e9 de Lyon \u2013 Unit\u00e9 Antibior\u00e9sistance et Virulence Bact\u00e9riennes","place":["Lyon, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","unstructured":"WHO. 2024. WHO bacterial priority pathogens list 2024: bacterial pathogens of public health importance to guide research development and strategies to prevent and control antimicrobial resistance. Available from: https:\/\/iris.who.int\/bitstream\/handle\/10665\/376776\/9789240093461-eng.pdf?sequence=1"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-019-0590-7"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics13121208"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.3389\/fpubh.2022.918658"},{"key":"e_1_3_4_6_2","unstructured":"Resapath. 2023. French surveillance network for antimicrobial resistance in bacteria from diseased animals. 2023 annual report. ANSES Lyon and Ploufragan-Plouzan\u00e9 France. Available from: https:\/\/resapath.anses.fr\/resapath_uploadfiles\/files\/Documents\/Rapport%20annuel\/2023_Resapath_Annual%20report.pdf"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2022-22362"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-12111-x"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2011.02543.x"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-60461-4"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.2903\/j.efsa.2017.4665"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2025.1552297"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11070940"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.3390\/ani14233383"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2022.01.010"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00792"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01358-21"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0248291"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2021.109049"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2024-26062"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.2903\/j.efsa.2017.4665"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0169589"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2542-5196(19)30130-5"},{"key":"e_1_3_4_24_2","unstructured":"Anses. 2020. Suivi des ventes de m\u00e9dicaments v\u00e9t\u00e9rinaires contenant des antibiotiques en France en 2019. Agence nationale de s\u00e9curit\u00e9 sanitaire de l\u2019alimentation de l\u2019environnement et du travail - Agence nationale du m\u00e9dicament v\u00e9t\u00e9rinaire Foug\u00e8res France"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.epidem.2020.100389"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkv485"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.936843"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00466-24"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02288"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00659-26","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00659-26","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T14:41:30Z","timestamp":1786027290000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.00659-26"}},"subtitle":[],"editor":[{"given":"Sadjia","family":"Bekal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,8,4]]},"references-count":28,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8,4]]}},"alternative-id":["10.1128\/spectrum.00659-26"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.00659-26","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,4]]},"assertion":[{"value":"2026-02-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00659-26"}}