{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T01:15:17Z","timestamp":1788225317075,"version":"build-2803163510"},"reference-count":65,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["U19AI166058"],"award-info":[{"award-number":["U19AI166058"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["U19AI166798"],"award-info":[{"award-number":["U19AI166798"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,2,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Sarocladium<\/jats:italic>\n                      is a fungal genus containing diverse members with roles as pathogens of plants and animals, endophytes, and biocontrol agents. This study characterizes two\n                      <jats:italic toggle=\"yes\">Sarocladium spinificis<\/jats:italic>\n                      strains isolated from patients diagnosed with coccidioidomycosis. Morphological, phylogenetic, and genomic analyses confirmed their classification within the\n                      <jats:italic toggle=\"yes\">Sarocladium<\/jats:italic>\n                      genus, closely related to\n                      <jats:italic toggle=\"yes\">S. spinificis<\/jats:italic>\n                      . Both strains exhibited mycelial growth at 24\u00b0C and 37\u00b0C, with distinct morphological features. Comparative genomic analysis identified unique orthologous clusters and functional gene families associated with diverse carbon utilization and potential pathogenicity. Notably, these strains\u2019 genomes contain many secreted proteins, carbohydrate-activated enzymes, and peptidases, highlighting their metabolic versatility. Co-cultivation experiments revealed that\n                      <jats:italic toggle=\"yes\">S. spinificis<\/jats:italic>\n                      strains CA16 and CA18 both inhibited the growth of\n                      <jats:italic toggle=\"yes\">Coccidioides posadasii<\/jats:italic>\n                      , suggesting antagonistic interactions and potential antifungal properties. These findings underscore the ecological and clinical significance of\n                      <jats:italic toggle=\"yes\">S. spinificis<\/jats:italic>\n                      and its impact on microbial communities, advancing our understanding of its diversity, pathogenic potential, and antifungal capabilities.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      Accurate identification and characterization of emerging fungal pathogens are essential for improving diagnosis and treatment.\n                      <jats:italic toggle=\"yes\">Sarocladium spinificis<\/jats:italic>\n                      , though not widely recognized as a human pathogen, exhibits traits suggesting a potential role in opportunistic infections. This study provides the first detailed morphological, phylogenetic, and genomic characterization of clinical\n                      <jats:italic toggle=\"yes\">S. spinificis<\/jats:italic>\n                      isolates, highlighting its ability to survive at body temperature and its antifungal resistance. Additionally, we demonstrate that these strains inhibit\n                      <jats:italic toggle=\"yes\">Coccidioides posadasii<\/jats:italic>\n                      , the agent of Valley fever, suggesting ecological competition or antifungal properties. These findings contribute to understanding fungal interactions in clinical and environmental settings, with implications for fungal pathogenesis and antifungal strategies. By uncovering new aspects of\n                      <jats:italic toggle=\"yes\">S. spinificis<\/jats:italic>\n                      biology, this work expands knowledge of\n                      <jats:italic toggle=\"yes\">Sarocladium<\/jats:italic>\n                      species in human health and lays the foundation for future research on its ecological role, pathogenic potential, and therapeutic applications.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.00689-25","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T13:00:56Z","timestamp":1767013256000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Morphological and genetic characterization of novel\n                    <i>Sarocladium spinificis<\/i>\n                    strains in association with\n                    <i>Coccidioides 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