{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T13:58:36Z","timestamp":1785419916605,"version":"3.56.0"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T00:00:00Z","timestamp":1754352000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T00:00:00Z","timestamp":1754352000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"crossref","award":["RS-2025-00553721"],"award-info":[{"award-number":["RS-2025-00553721"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"crossref"}]},{"name":"SNUBH Research Fund","award":["16-2023-0011"],"award-info":[{"award-number":["16-2023-0011"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,8,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Mycobacterium abscessus<\/jats:italic>\n                      presents significant clinical challenges due to its intrinsic and acquired resistance to antibiotics, resulting in prolonged treatments and poor patient outcomes. Addressing the urgent need for novel therapeutics, this study explores the antimicrobial potential of VLX600, originally developed as an anticancer agent, against\n                      <jats:italic toggle=\"yes\">M. abscessus<\/jats:italic>\n                      . Screening a library of 3,200 clinically evaluated compounds identified VLX600 as a potent antimicrobial with minimal cytotoxicity. VLX600 demonstrated inhibitory effects against various strains of\n                      <jats:italic toggle=\"yes\">M. abscessus<\/jats:italic>\n                      with minimum inhibitory concentrations of 4 \u00b5g\/mL\u201316 \u00b5g\/mL. It also remained effective in intracellular\n                      <jats:italic toggle=\"yes\">M. abscessus<\/jats:italic>\n                      in host cells and exhibited broad-spectrum activity against other bacterial species, including\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">Pseudomonas aeruginosa<\/jats:italic>\n                      . The antimicrobial activity of VLX600 was abrogated by supplemental iron, indicating a mechanism dependent on iron chelation. VLX600 significantly reduced bacterial burdens and inflammation in a murine model of pulmonary\n                      <jats:italic toggle=\"yes\">M. abscessus<\/jats:italic>\n                      infection. Additionally, synergistic effects were observed when VLX600 was combined with conventional antibiotics such as amikacin and clarithromycin\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      . These findings highlight VLX600 as a promising candidate for repurposing as an antimicrobial agent against\n                      <jats:italic toggle=\"yes\">M. abscessus<\/jats:italic>\n                      , warranting further clinical investigations.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        <jats:italic toggle=\"yes\">Mycobacterium abscessus<\/jats:italic>\n                        is an opportunistic pathogen that commonly causes pulmonary infections in cystic fibrosis patients. These infections are notoriously difficult to treat due to high levels of antibiotic resistance of\n                        <jats:italic toggle=\"yes\">M. abscessus<\/jats:italic>\n                        , resulting in low cure rates. In this study, we identified a novel antibiotic candidate, VLX600, through high-throughput screening of 3,200 clinical compounds and demonstrated that VLX600 inhibits the growth of\n                        <jats:italic toggle=\"yes\">M. abscessus<\/jats:italic>\n                        by depriving it of ferric and ferrous ions. This study highlights the potential of iron chelators as antimicrobial agents against\n                        <jats:italic toggle=\"yes\">M. abscessus<\/jats:italic>\n                        infections. Since iron is an essential nutrient for the growth of many bacteria, the use of iron chelators could be extended to other infectious diseases. We hope this research will inspire further studies aimed at developing iron chelators as a novel class of antimicrobial agents.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.00719-25","type":"journal-article","created":{"date-parts":[[2025,6,20]],"date-time":"2025-06-20T09:00:57Z","timestamp":1750410057000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["VLX600, an anticancer iron chelator, exerts antimicrobial effects on\n                    <i>Mycobacterium abscessus<\/i>\n                    infections"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-8659-8636","authenticated-orcid":false,"given":"Jaehun","family":"Oh","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04h9pn542","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and 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