{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T00:44:12Z","timestamp":1784335452455,"version":"3.55.0"},"reference-count":20,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2022,8,31]],"date-time":"2022-08-31T00:00:00Z","timestamp":1661904000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2022,8,31]],"date-time":"2022-08-31T00:00:00Z","timestamp":1661904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32161133005"],"award-info":[{"award-number":["32161133005"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2022,8,31]]},"abstract":"<jats:p>\n            Emergence of pathogens harboring tigecycline resistance genes incurs great concerns. Wastewater is recognized as the important reservoir of antimicrobial resistance genes. Here we characterized the phenotypes and genotypes of bacteria carrying\n            <jats:italic>tet<\/jats:italic>\n            (X4) from wastewater in Turkey for the first time. Four\n            <jats:italic>tet<\/jats:italic>\n            (X4)-positive\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            isolates were identified and characterized by PCR, Sanger sequencing, antimicrobial susceptibility testing, conjugation assays, Illumina sequencing, nanopore sequencing and bioinformatic analysis. Four\n            <jats:italic>tet<\/jats:italic>\n            (X4)-harboring isolates were multidrug-resistant (MDR) bacteria and the\n            <jats:italic>tet<\/jats:italic>\n            (X4) gene was nontransferable in four isolates. Genetic analysis revealed that\n            <jats:italic>tet<\/jats:italic>\n            (X4) genes in four isolates were located on plasmids co-harboring two replicons IncFIA(HI1) and IncFIB(K). However, none of the four plasmids carried genes associated with horizontal transfer of plasmids. The coexistence of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>SHV-12<\/jats:sub>\n            -bearing IncX3-type plasmid and\n            <jats:italic>tet<\/jats:italic>\n            (X4)-harboring plasmid was also found in one isolate. These findings indicate that continuous surveillance of the\n            <jats:italic>tet<\/jats:italic>\n            (X4)-bearing isolates in different environments worldwide should be strengthened.\n          <\/jats:p>","DOI":"10.1128\/spectrum.00732-22","type":"journal-article","created":{"date-parts":[[2022,7,13]],"date-time":"2022-07-13T13:02:51Z","timestamp":1657717371000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["Emergence and Characterization of Tigecycline Resistance Gene\n            <i>tet<\/i>\n            (X4) in ST609 Escherichia coli Isolates from Wastewater in Turkey"],"prefix":"10.1128","volume":"10","author":[{"given":"Cemil","family":"K\u00fcrekci","sequence":"first","affiliation":[{"name":"Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, Hatay, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1330-5925","authenticated-orcid":true,"given":"Xiaoyu","family":"Lu","sequence":"additional","affiliation":[{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu Province, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00fcsra G\u00fclay","family":"Celil","sequence":"additional","affiliation":[{"name":"Graduate School of Health Sciences, Hatay Mustafa Kemal University, Hatay, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00fcseyin Burak","family":"Disli","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, Hatay, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mashkoor","family":"Mohsin","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, University of Agriculture, Faisalabad, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6486-9600","authenticated-orcid":true,"given":"Zhiqiang","family":"Wang","sequence":"additional","affiliation":[{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu Province, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8333-9891","authenticated-orcid":true,"given":"Ruichao","family":"Li","sequence":"additional","affiliation":[{"name":"Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu Province, P. R. China"},{"name":"Institute of Comparative Medicine, Yangzhou University, Yangzhou, Jiangsu Province, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2018.07.003"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1592\/phco.26.8.1099"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-019-0445-2"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-019-0496-4"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2021.07.019"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2017.09.005"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2021.117233"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msp077"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.01221-20"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00134-20"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkr174"},{"key":"e_1_3_2_14_2","article-title":"Widespread prevalence and molecular epidemiology of tet(X4) and mcr-1 harboring Escherichia coli isolated from chickens in Pakistan","author":"Li R","year":"2021","unstructured":"Li R, Lu X, Munir A, Abdullah S, Liu Y, Xiao X, Wang Z, Mohsin M. 2021. Widespread prevalence and molecular epidemiology of tet(X4) and mcr-1 harboring Escherichia coli isolated from chickens in Pakistan. Sci Total Environ","journal-title":"Sci Total Environ"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1005595"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/gix132"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01629-21"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.14252\/foodsafetyfscj.2017011"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00699-19"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.02002"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2021.02.010"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00732-22","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00732-22","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,31]],"date-time":"2022-08-31T14:18:47Z","timestamp":1661955527000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.00732-22"}},"subtitle":[],"editor":[{"given":"Monica Adriana","family":"Garcia-Solache","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2022,8,31]]},"references-count":20,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,8,31]]}},"alternative-id":["10.1128\/spectrum.00732-22"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.00732-22","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,31]]},"assertion":[{"value":"2022-02-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-06-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00732-22"}}