{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T16:30:41Z","timestamp":1775147441311,"version":"3.50.1"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T00:00:00Z","timestamp":1730764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,11,5]],"date-time":"2024-11-05T00:00:00Z","timestamp":1730764800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["82150410458"],"award-info":[{"award-number":["82150410458"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,11,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Biofilms formed by\n              <jats:italic>Escherichia coli<\/jats:italic>\n              are composed of amyloid curli and cellulose and have been shown to be linked to pathogenicity, antibiotic resistance, and chronic infections. Guanabenz acetate (GABE), an antihypertensive drug, was identified as a potential strategic repurposing drug due to its biofilm inhibitory properties following an extensive antimicrobial screening assay of 2,202 Food and Drug Administration-approved non-antibiotic agents. The results of this study provide insights into the effectiveness of GABE as a therapeutic alternative against\n              <jats:italic>E. coli<\/jats:italic>\n              biofilm-associated infectious diseases.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Biofilm-associated bacterial infections are one of the major problems in medical settings. There are currently limited biofilm inhibitors available for clinical use. Guanabenz acetate, a drug used to treat high blood pressure, was found to be an effective anti-biofilm agent against\n              <jats:italic>Escherichia coli<\/jats:italic>\n              . Our results show that this drug can inhibit the production of cellulose and curli amyloid protein, which are the two main components of\n              <jats:italic>E. coli<\/jats:italic>\n              biofilms. Our findings highlight the possibility of repurposing a drug to prevent\n              <jats:italic>E. coli<\/jats:italic>\n              biofilm formation.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.00738-24","type":"journal-article","created":{"date-parts":[[2024,9,27]],"date-time":"2024-09-27T05:18:36Z","timestamp":1727414316000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Guanabenz acetate, an antihypertensive drug repurposed as an inhibitor of\n            <i>Escherichia coli<\/i>\n            biofilm"],"prefix":"10.1128","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7270-1461","authenticated-orcid":true,"given":"Arakkaveettil Kabeer","family":"Farha","sequence":"first","affiliation":[{"name":"Biotechnology and Food Engineering Program; and Key Laboratory of Science and Engineering for Health and Medicine of Guangdong Higher Education Institutes, Guangdong Technion-Israel Institute of Technology, Shantou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Habimana","sequence":"additional","affiliation":[{"name":"Biotechnology and Food Engineering Program; and Key Laboratory of Science and Engineering for Health and Medicine of Guangdong Higher Education Institutes, Guangdong Technion-Israel Institute of Technology, Shantou, China"},{"name":"Faculty of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harold","family":"Corke","sequence":"additional","affiliation":[{"name":"Biotechnology and Food Engineering Program; and Key Laboratory of Science and Engineering for Health and Medicine of Guangdong Higher Education Institutes, Guangdong Technion-Israel Institute of Technology, Shantou, China"},{"name":"Faculty of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41522-021-00251-2"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics10010003"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00028-18"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.60.080805.142106"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.15216"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA119.011668"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-198326030-00003"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.4132\/jptm.2019.01.14"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-70689-5"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-021-03570-0"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01113-20"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0089580"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.7.4022-4034.2005"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.3934\/microbiol.2017.4.915"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01616-18"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.7.2178-2186.2001"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-021-03080-2"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-010-9862-4"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2013.12.030"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00510-20"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10061103"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11060816"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/2049-632X.12141"},{"key":"e_1_3_4_25_2","first-page":"539","article-title":"In vitro and in vivo antimycobacterial activity of an antihypertensive agent methyl-L-DOPA","volume":"19","author":"Dutta NK","year":"2005","unstructured":"Dutta NK, Mazumdar K, Dastidar SG, Chakrabarty AN, Shirataki Y, Motohashi N. 2005. In vitro and in vivo antimycobacterial activity of an antihypertensive agent methyl-L-DOPA. 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