{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:52:33Z","timestamp":1785430353596,"version":"3.56.0"},"reference-count":45,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Recent work has optimized parameters of a real-time reverse transcriptase PCR-based laboratory-developed test on the Panther Fusion system which detects Mycoplasma genitalium-specific macrolide resistance-associated mutations LDT (MRM-LDT) from primary swab and urine specimens. In this study, MRM-LDT was applied to a large multi-demographic study set to further characterize\n                      <jats:italic toggle=\"yes\">M. genitalium<\/jats:italic>\n                      resistance in the United States. A total of 2,145 primary clinical specimens testing positive for\n                      <jats:italic toggle=\"yes\">M. genitalium<\/jats:italic>\n                      16S rRNA by transcription-mediated amplification (TMA) were initially titered by the same assay using serial 10-fold dilutions to determine relative target nucleic acid burden. Specimens were then processed for MRM-LDT. Findings were stratified by men who have sex with men (MSM;\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 3 settings), community care (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 2), and university student (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 3) populations. The mean log\n                      <jats:sub>10<\/jats:sub>\n                      target nucleic acid titer of a TMA-positive specimen was 3.46 (median 3; range 0\u201310). A 43.2% MRM-LDT detection rate was found in specimens derived from community care settings. Analogous assessments of university student and MSM demographics revealed 60.8% and 62.0% detection, respectively (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      \u2264 0.0004 versus community care). Within the university student demographic, differences existed in target nucleic acid titer for two settings (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.01); similar differences were encountered between a local MSM cohort and two that were nationally based (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      \u2264 0.01). Within the university cohort, MRM-LDT detection rate was increased in symptomatic patients (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = 0.005).\n                      <jats:italic toggle=\"yes\">M. genitalium<\/jats:italic>\n                      macrolide resistance rates among multiple demographics, as determined by MRM-LDT, are high in the United States and are consistent with target nucleic acid burden within the primary specimen. However, caveats experienced within subgroupings of these demographics support reflex MRM-LDT on\n                      <jats:italic toggle=\"yes\">M. genitalium<\/jats:italic>\n                      -positive specimens.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      Data generated from a high-throughput, automated system and presented in this report expand upon knowledge of\n                      <jats:italic toggle=\"yes\">Mycoplasma genitalium<\/jats:italic>\n                      -specific macrolide resistance in the United States and may further inform providers on population- or demographic-based considerations for macrolide resistance mutation determination in\n                      <jats:italic toggle=\"yes\">M. genitalium<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.00738-25","type":"journal-article","created":{"date-parts":[[2025,5,22]],"date-time":"2025-05-22T09:00:43Z","timestamp":1747904443000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Automated\n                    <i>Mycoplasma genitalium<\/i>\n                    molecular macrolide resistance detection and nucleic acid target semi-quantitation: patient demographic considerations"],"prefix":"10.1128","volume":"13","author":[{"given":"Josephine","family":"Moore","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04gr4te78","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medical Laboratory Science, Marquette University","place":["Milwaukee, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trinity","family":"Krueger","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04gr4te78","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medical Laboratory Science, Marquette University","place":["Milwaukee, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda","family":"Zapp","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04gr4te78","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medical Laboratory Science, Marquette University","place":["Milwaukee, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen C.","family":"Lavey","sequence":"additional","affiliation":[{"name":"Loyola University Parkinson School of Health Sciences and Public Health","place":["Maywood, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimber L.","family":"Munson","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04gr4te78","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medical Laboratory Science, Marquette University","place":["Milwaukee, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene A.","family":"Stafford","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, McGovern Medical School at UTHealth","place":["Houston, USA"]},{"name":"Division of Maternal Fetal Medicine, McGovern Medical School at UTHealth","place":["Houston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael E.","family":"Newcomb","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Northwestern University","place":["Chicago, USA"]},{"id":[{"id":"https:\/\/ror.org\/000e0be47","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medicine, Northwestern University","place":["Evanston, USA"]},{"id":[{"id":"https:\/\/ror.org\/000e0be47","id-type":"ROR","asserted-by":"publisher"}],"name":"Feinberg School of Medicine, Northwestern University","place":["Evanston, USA"]},{"id":[{"id":"https:\/\/ror.org\/000e0be47","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute for Sexual and Gender Minority Health and Wellbeing, Northwestern University","place":["Chicago, USA"]},{"id":[{"id":"https:\/\/ror.org\/000e0be47","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medical Social Services, Northwestern University","place":["Chicago, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Mustanski","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Northwestern University","place":["Chicago, USA"]},{"id":[{"id":"https:\/\/ror.org\/000e0be47","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medicine, Northwestern University","place":["Evanston, USA"]},{"id":[{"id":"https:\/\/ror.org\/000e0be47","id-type":"ROR","asserted-by":"publisher"}],"name":"Feinberg School of Medicine, Northwestern University","place":["Evanston, USA"]},{"id":[{"id":"https:\/\/ror.org\/000e0be47","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute for Sexual and Gender Minority Health and Wellbeing, Northwestern University","place":["Chicago, USA"]},{"id":[{"id":"https:\/\/ror.org\/000e0be47","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medical Social Services, Northwestern University","place":["Chicago, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5359-6643","authenticated-orcid":true,"given":"Erik","family":"Munson","sequence":"additional","affiliation":[{"name":"Wisconsin Clinical Laboratory Network Laboratory Technical Advisory Group","place":["Madison, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00790-21"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00818-17"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02124-19"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-020-04135-9"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01125-19"},{"key":"e_1_3_2_7_2","unstructured":"Centers for Disease Control and Prevention. Antibiotic resistance threats in the United States 2019. https:\/\/www.cdc.gov\/drugresistance \/pdf\/threats-report\/2019-ar-threats-report-508.pdf."},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciu1162"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0000000000000631"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.rr7004a1"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.03654-22"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.01006-24"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkac281"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2018.02.002"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-937-2_8"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00020-21"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01900-19"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00335-23"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1080\/07448481.2021.1899185"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.3121\/cmr.2024.1871"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2015.03.016"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02463-15"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2021-050475"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.2013.814429"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0000000000005642"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1097\/QAI.0000000000001886"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0000000000001244"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01625-16"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0000000000001911"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmii.2024.05.004"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1097\/LGT.0000000000000828"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.53854\/liim-3202-10"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.25259\/IJDVL_933_2023"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.37201\/req\/015.2024"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.3390\/genes15040470"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2023-056044"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2023-056093"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciad405"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofae407"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa1199"},{"key":"e_1_3_2_41_2","unstructured":"Munson E Zapp A Russell H Moore J Lavey S Munson K Stafford I Mustanski B. 2023. Clinical and epidemiologic correlates of Mycoplasmoides genitalium macrolide resistance and relative specimen nucleic acid target burden in the United States as determined by automated commercial assays. Houston Texas ASM Microbe"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/apm.13477"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00485-24"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00819-21"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2020-054463"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofae444"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00738-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00738-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T09:05:56Z","timestamp":1751360756000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.00738-25"}},"subtitle":[],"editor":[{"given":"S. Wesley","family":"Long","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":45,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1128\/spectrum.00738-25"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.00738-25","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"2025-03-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00738-25"}}