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This study investigated the plasmid content of 329\n                      <jats:italic toggle=\"yes\">S<\/jats:italic>\n                      .\n                      <jats:italic toggle=\"yes\">aureus<\/jats:italic>\n                      isolates from livestock and companion animals collected in France between 2010 and 2021. Plasmids (\n                      <jats:italic toggle=\"yes\">n<\/jats:italic>\n                      = 211) were identified from 139 isolates. The major families identified\u2014\n                      <jats:italic toggle=\"yes\">rep7a<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">rep20<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">rep10<\/jats:italic>\n                      \u2014were associated with specific resistance genes (\n                      <jats:italic toggle=\"yes\">str<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">cat<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">blaZ<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">erm<\/jats:italic>\n                      (C)) and exhibited widespread horizontal transfer across different\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      sequence types (STs) and animal hosts. In temporal analysis, the\n                      <jats:italic toggle=\"yes\">rep7a<\/jats:italic>\n                      \/str and\n                      <jats:italic toggle=\"yes\">rep7a<\/jats:italic>\n                      \/\n                      <jats:italic toggle=\"yes\">cat<\/jats:italic>\n                      plasmids circulating in horses were progressively replaced by a\n                      <jats:italic toggle=\"yes\">rep7a<\/jats:italic>\n                      plasmid carrying both\n                      <jats:italic toggle=\"yes\">str<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">cat<\/jats:italic>\n                      genes. The study also highlighted the presence of mosaic plasmids, which combined elements from different bacterial species\/genera, confirming the broad host range of\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      plasmids and their ability to acquire ARGs from diverse sources. Moreover, the occurrence of hybrid plasmids (carrying multiple\n                      <jats:italic toggle=\"yes\">rep<\/jats:italic>\n                      genes) underscores the plasticity of these vectors of ARGs. This study emphasizes the need to investigate the mechanisms driving the spread and persistence of antibiotic-resistant plasmids in\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      , with a view to developing strategies aimed at combating antibiotic resistance.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      The spread of antibiotic resistance in\n                      <jats:italic toggle=\"yes\">Staphylococcus aureus<\/jats:italic>\n                      is a growing concern, particularly in animals that can serve as reservoirs for resistant strains. This study highlights the crucial role of plasmids in transmitting resistance genes among different animal hosts and\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      lineages. The characterization of 329 isolates collected over 10 years revealed how certain plasmid families are associated with specific resistance genes and how they evolve over time. The occurrence of mosaic and hybrid plasmids further underscores the ability of\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      to acquire resistance from diverse bacterial sources. These findings provide key insights into the mechanisms shaping antibiotic resistance in this pathogen and emphasize the fact that understanding plasmid-driven resistance is essential for developing effective interventions to limit the spread of multidrug-resistant\n                      <jats:italic toggle=\"yes\">S. aureus<\/jats:italic>\n                      in both veterinary and human medicine.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.00772-25","type":"journal-article","created":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T14:56:56Z","timestamp":1758207416000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Comprehensive genomic analysis of antibiotic resistance plasmids in animal-associated\n                    <i>Staphylococcus aureus<\/i>\n                    in 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