{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:51:13Z","timestamp":1786117873238,"version":"build-2736575974"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100008771","name":"Animal and Plant Quarantine Agency","doi-asserted-by":"publisher","award":["N-1543081-2017-36-01"],"award-info":[{"award-number":["N-1543081-2017-36-01"]}],"id":[{"id":"10.13039\/501100008771","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2026,8,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Streptococcus uberis<\/jats:italic>\n                      , a major environmental pathogen, is frequently linked to recurrent or persistent intramammary infections, posing a challenge to mastitis control in dairy cattle. In this study, we characterized 112\n                      <jats:italic toggle=\"yes\">S. uberis<\/jats:italic>\n                      isolates recovered from bovine mastitis milk samples collected across South Korea between 2019 and 2024. Multilocus sequence typing revealed substantial genetic diversity, with all sequence types being newly assigned, and no evidence of a dominant clonal lineage. High levels of resistance to tetracycline (81.3%), erythromycin (75.9%), and pirlimycin (75.0%) were observed. Notably, 74.1% of the isolates were classified as multidrug-resistant. The corresponding resistance genes\n                      <jats:italic toggle=\"yes\">tetO<\/jats:italic>\n                      (82.1%) and\n                      <jats:italic toggle=\"yes\">ermB<\/jats:italic>\n                      (79.5%) were commonly detected and showed strong concordance with resistance phenotypes. Virulence-associated genes, including\n                      <jats:italic toggle=\"yes\">sua<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">mtuA<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">oppF<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">skc<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">pauA<\/jats:italic>\n                      , were widely detected (89.3\u2013100%). All isolates were capable of biofilm formation, although the majority demonstrated weak biofilm-formation ability. This study provides important insights into the epidemiology and pathogenic potential of bovine mastitis-associated\n                      <jats:italic toggle=\"yes\">S. uberis<\/jats:italic>\n                      isolates in South Korea and underscores the need for continued surveillance and prudent antimicrobial use to formulate effective mastitis-control strategies.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Streptococcus uberis<\/jats:italic>\n                      is a leading environmental cause of bovine mastitis worldwide, yet region-specific molecular epidemiological data remain limited in South Korea. To our knowledge, this study represents the first nationwide molecular characterization of mastitis-associated\n                      <jats:italic toggle=\"yes\">S. uberis<\/jats:italic>\n                      isolates in South Korea, integrating MLST-based population analysis, antimicrobial susceptibility testing, resistance gene detection, and virulence profiling. The identification of exclusively novel sequence types indicates substantial genetic diversity within the regional population. The high prevalence of tetracycline and MLS-type resistance, closely associated with\n                      <jats:italic toggle=\"yes\">tetO<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">ermB<\/jats:italic>\n                      , highlights the importance of continued monitoring of antimicrobial resistance in the dairy production. Additionally, the widespread detection of core virulence-associated genes and universal biofilm-forming capacity indicates conserved characteristics among mastitis-associated isolates. These findings provide baseline data to support future surveillance and control strategies targeting bovine mastitis-associated\n                      <jats:italic toggle=\"yes\">S. uberis<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.00772-26","type":"journal-article","created":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T13:03:25Z","timestamp":1782133405000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Molecular and phenotypic characterization of\n                    <i>Streptococcus uberis<\/i>\n                    isolates from bovine mastitis milk in South Korea"],"prefix":"10.1128","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4478-0147","authenticated-orcid":false,"given":"Hye Jeong","family":"Kang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04sbe6g90","id-type":"ROR","asserted-by":"publisher"}],"name":"Bacterial Disease Division, Animal and Plant Quarantine Agency","place":["Gimcheon-si, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ju-Yeon","family":"You","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04sbe6g90","id-type":"ROR","asserted-by":"publisher"}],"name":"Bacterial Disease Division, Animal and Plant Quarantine Agency","place":["Gimcheon-si, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung Hoe","family":"Kim","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04sbe6g90","id-type":"ROR","asserted-by":"publisher"}],"name":"Bacterial Disease Division, Animal and Plant Quarantine Agency","place":["Gimcheon-si, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun Sang","family":"Cho","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04sbe6g90","id-type":"ROR","asserted-by":"publisher"}],"name":"Bacterial Disease Division, Animal and Plant Quarantine Agency","place":["Gimcheon-si, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-6337-5002","authenticated-orcid":true,"given":"Hyun-Mi","family":"Kang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04sbe6g90","id-type":"ROR","asserted-by":"publisher"}],"name":"Bacterial Disease Division, Animal and Plant Quarantine Agency","place":["Gimcheon-si, Republic of Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00284-015-0958-8"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.5713\/ajas.20.0156"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.12704"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-015-1512-6"},{"key":"e_1_3_4_6_2","volume-title":"Veterinary microbiology and microbial disease","author":"Quinn PJ","year":"2025","unstructured":"Quinn PJ. 2025. 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