{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T13:46:19Z","timestamp":1787579179748,"version":"build-2736575974"},"reference-count":77,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,7]],"date-time":"2025-10-07T00:00:00Z","timestamp":1759795200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,10,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Senegal has witnessed the emergence and spread of carbapenem-resistant\n                      <jats:italic toggle=\"yes\">Enterobacterales<\/jats:italic>\n                      (CRE), which often cause deadly infections. Accordingly, this study aimed to determine the antimicrobial susceptibility and prevalence of carbapenemases, as well as to perform a whole-genome sequence analysis of clinical CRE isolates from a university hospital in Dakar, Senegal. MALDI-TOF MS and VITEK2 systems were used for bacterial identification and antimicrobial susceptibility testing (AST). Carbapenemase- and cephalosporinase-encoding genes were screened using simplex end-point polymerase chain reaction. Whole-genome sequencing (WGS) was performed using the Illumina MiSeq platform. The CRE isolates were resistant to almost all the 34 antimicrobials tested. Nevertheless, colistin and amikacin remained active, with susceptibility rates of 96% and 71%, respectively. Only the carbapenemase genes\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>OXA-48<\/jats:sub>\n                      (53.8%; 15\/28) and\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>NDM<\/jats:sub>\n                      (35.7%; 10\/28) and the cephalosporinase gene\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>CMY-1<\/jats:sub>\n                      (25%; 7\/28) were identified. In this context, two extensively drug-resistant\n                      <jats:italic toggle=\"yes\">Enterobacter hormaechei<\/jats:italic>\n                      isolates were subjected to WGS analysis. These isolates were assigned as sequence type (ST) 182 and carried several genes related to antimicrobial resistance (AMR), metal tolerance, and virulence. An IncL\/M plasmid with 61,054 bp in length was identified as carrying the\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>OXA-48<\/jats:sub>\n                      gene, whereas an IncFIB(pECLA)\/IncFII(pECLA)\/IncX3 mutireplicon plasmid with 217,745 bp in length was detected as harboring the\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>NDM-5<\/jats:sub>\n                      gene and other genes related to AMR and metal tolerance. Our study presents the first landscape of clinical CRE circulating in Senegal, along with additional genomic analysis of\n                      <jats:italic toggle=\"yes\">E. hormaechei<\/jats:italic>\n                      ST182 strains, which could be useful for mitigating the burden associated with CRE in this country.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        The investigation of global critical priority CRE isolates has become crucial to reduce morbidity and mortality associated with AMR. This study revealed that colistin and amikacin can be considered good alternatives for treating CRE-associated infections in Dakar. In addition, the genomic approach revealed that the CRE isolates carried both a wide resistome and virulome. Moreover, the abundance of horizontal gene transfer regions in the genomes suggests the great implications of mobile genetic elements in the spread of AMR in Dakar. Furthermore, this study reported the complete sequences of chromosomes and\n                        <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                        <jats:sub>OXA-48<\/jats:sub>\n                        and\n                        <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                        <jats:sub>NDM-5<\/jats:sub>\n                        -carrying plasmids. Our findings are of great importance because complete genome sequences are still rarely characterized in the West African region. Finally, this study highlights the importance of strengthening genomic surveillance of CRE in sub-Saharan African countries to mitigate the burden associated with these pathogens.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.00780-25","type":"journal-article","created":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T13:00:12Z","timestamp":1758027612000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Clinical carbapenem-resistant\n                    <i>Enterobacterales<\/i>\n                    in a University Hospital in Dakar, Senegal: genomic insights into\n                    <i>Enterobacter hormaechei<\/i>\n                    ST182 strains carrying\n                    <i>bla<\/i>\n                    <sub>NDM-5<\/sub>\n                    and\n                    <i>bla<\/i>\n                    <sub>OXA-48<\/sub>\n                    genes"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0967-5314","authenticated-orcid":true,"given":"Komla Mawunyo","family":"Dossouvi","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04fp9fm22","id-type":"ROR","asserted-by":"publisher"}],"name":"Bacteriology-Virology laboratory, National University Hospital Aristide Le Dantec","place":["Dakar, Senegal"]},{"name":"Department of Microbiology, Global Health Research Institute","place":["Lom\u00e9, Togo"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bissoume","family":"Sambe Ba","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01f80g185","id-type":"ROR","asserted-by":"publisher"}],"name":"WHO Country Office Senegal, World Health Organization","place":["Dakar, 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