{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T15:50:42Z","timestamp":1785167442664,"version":"3.55.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              Urinary tract infections (UTIs) are the most common bacterial infections in humans. They are mainly caused by\n              <jats:italic>Escherichia coli<\/jats:italic>\n              and other Enterobacterales for which increasing resistance to antibiotics and in particular to \u03b2-lactams is extensively reported. The detection of \u03b2-lactam resistance phenotypes is currently time-consuming (18 h). Hence, most treatments are given without any results of antibiotic susceptibility testing and may involve broad-spectrum antibiotics. A biochemical diagnostic test has been developed to rapidly evaluate the production of \u03b2-lactamases (and consequently the \u03b2-lactam resistance) from cultures (10\n              <jats:sup>4<\/jats:sup>\n              and 10\n              <jats:sup>5<\/jats:sup>\n              CFU\/mL) of Gram-positive and Gram-negative isolates representative of bacterial species as a source of UTIs (\n              <jats:italic>n<\/jats:italic>\n              = 112). It relies on a centrifugation step after a 90 min preliminary culture and the detection of \u03b2-lactamase activity with nitrocefin as substrate using a special filter. Overall, the test gave a positive result for 87.6% of the tested resistant strains with a bacterial load of 10\n              <jats:sup>5<\/jats:sup>\n              CFU\/mL, and a positive result for 100% of tested extended-spectrum \u03b2-lactamases and for carbapenemase producers at the same load. This cost-effective test can be performed in any laboratory and ultimately shall be tested at the patient side and at the general practitioner. Its turn-around-time to get results is less than 2 h. After further improvements, the results obtained with this proof-of-concept test suggest that its use may contribute to rapidly guide the treatment of non-complicated UTIs and, therefore, limit the use of broad-spectrum antibiotics and the emergence of antibiotic resistance.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>This work reports on a totally novel diagnostic technique, the Rapid Amp NP test for the identification of amoxicillin\/ampicillin resistance in bacteria that are sources of non-complicated urinary tract infections. Those preliminary results obtained with cultured bacteria are promising. We believe its future use may contribute to reconsider aminopenicillins as a first line therapy for treating UTI infections. The corresponding patent of this test obtained both for the United States and Europe may contribute to its further industrialization.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.00782-24","type":"journal-article","created":{"date-parts":[[2025,3,6]],"date-time":"2025-03-06T14:10:04Z","timestamp":1741270204000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Rapid detection of \u03b2-lactamase activity using the rapid Amp NP test"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1343-1622","authenticated-orcid":false,"given":"Patrice","family":"Nordmann","sequence":"first","affiliation":[{"name":"European Institute for Emerging Antibiotic Resistance, University of Fribourg","place":["Fribourg, Switzerland"]},{"name":"Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg","place":["Fribourg, Switzerland"]},{"name":"Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg","place":["Fribourg, Switzerland"]},{"name":"Institute for Microbiology, University Hospital and University of Lausanne","place":["Lausanne, Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8902-0486","authenticated-orcid":true,"given":"Nicolas","family":"Helsens","sequence":"additional","affiliation":[{"name":"European Institute for Emerging Antibiotic Resistance, University of Fribourg","place":["Fribourg, Switzerland"]},{"name":"Clinical Microbiology Unit, Pasteur Institute of Lille","place":["Lille, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Kieffer","sequence":"additional","affiliation":[{"name":"Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg","place":["Fribourg, Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camille","family":"Tinguely","sequence":"additional","affiliation":[{"name":"Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg","place":["Fribourg, Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9529-3317","authenticated-orcid":true,"given":"Gilbert","family":"Greub","sequence":"additional","affiliation":[{"name":"Institute for Microbiology, University Hospital and University of Lausanne","place":["Lausanne, Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5160-5286","authenticated-orcid":true,"given":"Laurent","family":"Poirel","sequence":"additional","affiliation":[{"name":"European Institute for Emerging Antibiotic Resistance, University of Fribourg","place":["Fribourg, Switzerland"]},{"name":"Medical and Molecular Microbiology, Faculty of Science and Medicine, University of Fribourg","place":["Fribourg, Switzerland"]},{"name":"Swiss National Reference Center for Emerging Antibiotic Resistance (NARA), University of Fribourg","place":["Fribourg, Switzerland"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"The Review on Antimicrobial Resistance (Chaired by Jim O\u2019Neill). 2016 Tackling drug- resistant infections globally: final report and recommendations. https:\/\/amr-review.org\/."},{"key":"e_1_3_2_3_2","unstructured":"World Health Organization. 2019. 2019 Antibacterial agents in clinical development: an analysis of the antibacterial clinical development pipeline. Available from: https:\/\/www.who.int\/publications\/i\/item\/9789240000193"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.h6420"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(08)70041-0"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25061340"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciz824"},{"key":"e_1_3_2_8_2","unstructured":"European Center for Disease Prevention and Control. 2019. Surveillance of antimicrobial resistance in Europe 2018. https:\/\/www.ecdc.europa.eu\/en\/publications-273 data\/surveillance-antimicrobial-resistance-europe-2018."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2018.10.013"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.ARBA-0026-2017"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00417"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.3389\/fpubh.2022.888205"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3432"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-017-1080-6"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1080\/14787210.2022.1990040"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13353-020-00542-y"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2019.06.016"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01578-14"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12913-021-06653-4"},{"key":"e_1_3_2_20_2","unstructured":"European Association of Urology. 2022. EAU (European Association of Urology) guidelines on urological infections. https:\/\/uroweb.org\/guidelines\/urological-infections."},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00859-12"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1809.120355"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00932-20"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-015-2560-1"},{"key":"e_1_3_2_25_2","unstructured":"Centers for Disease Control and Prevention. 2024. National healthcare safety network urinary tract infection (Catheter-Associated Urinary Tract Infection [CAUTI] and non catheter-associated urinary tract infection [UTI]) Events"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.1.4.283"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki482"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkab032"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03068-13"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1080\/00325481.2018.1398049"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.22301"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics12061042"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1039\/D1AN02098F"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0017146"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.858777"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac504428t"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2017-3239"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00788-21"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02273-19"}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00782-24","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00782-24","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T13:13:06Z","timestamp":1743513186000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.00782-24"}},"subtitle":[],"editor":[{"given":"Nilton","family":"Lincopan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,4]]},"references-count":38,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1128\/spectrum.00782-24"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.00782-24","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4]]},"assertion":[{"value":"2024-06-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00782-24"}}