{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T13:02:23Z","timestamp":1753880543027,"version":"3.41.2"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T00:00:00Z","timestamp":1722902400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T00:00:00Z","timestamp":1722902400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"name":"HHS | NIH | Fogarty International Center"},{"name":"Ministry of Science and Technology, Government of the People's Republic of Bangladesh"},{"name":"Canadian Government | Global Affairs Canada"},{"DOI":"10.13039\/100004441","name":"Swedish International Development Cooperation Agency","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100004441","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Canadian Government | Canadian Institutes of Health Research"},{"name":"UK Department for International Development"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2024,8,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              <jats:italic>Vibrio cholerae<\/jats:italic>\n              O1 causes the diarrheal disease cholera, and the small intestine is the site of active infection. During cholera, cholera toxin is secreted from\n              <jats:italic>V. cholerae<\/jats:italic>\n              and induces a massive fluid influx into the small intestine, which causes vomiting and diarrhea. Typically,\n              <jats:italic>V. cholerae<\/jats:italic>\n              genomes are sequenced from bacteria passed in stool, but rarely from vomit, a fluid that may more closely represents the site of active infection. We hypothesized that\n              <jats:italic>V. cholerae<\/jats:italic>\n              O1 population bottlenecks along the gastrointestinal tract would result in reduced genetic variation in stool compared to vomit. To test this, we sequenced\n              <jats:italic>V. cholerae<\/jats:italic>\n              genomes from 10 cholera patients with paired vomit and stool samples. Genetic diversity was low in both vomit and stool, consistent with a single infecting population rather than coinfection with divergent\n              <jats:italic>V. cholerae<\/jats:italic>\n              O1 lineages. The amount of single-nucleotide variation decreased from vomit to stool in four patients, increased in two, and remained unchanged in four. The variation in gene presence\/absence decreased between vomit and stool in eight patients and increased in two. Pangenome analysis of assembled short-read sequencing demonstrated that the toxin-coregulated pilus operon more frequently contained deletions in genomes from vomit compared to stool. However, these deletions were not detected by PCR or long-read sequencing, indicating that interpreting gene presence or absence patterns from short-read data alone may be incomplete. Overall, we found that\n              <jats:italic>V. cholerae<\/jats:italic>\n              O1 isolated from stool is genetically similar to\n              <jats:italic>V. cholerae<\/jats:italic>\n              recovered from the upper intestinal tract.\n            <\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              <jats:italic>Vibrio cholerae<\/jats:italic>\n              O1, the bacterium that causes cholera, is ingested in contaminated food or water and then colonizes the upper small intestine and is excreted in stool. Shed\n              <jats:italic>V. cholerae<\/jats:italic>\n              genomes from stool are usually studied, but\n              <jats:italic>V. cholerae<\/jats:italic>\n              isolated from vomit may be more representative of where\n              <jats:italic>V. cholerae<\/jats:italic>\n              colonizes in the upper intestinal epithelium.\n              <jats:italic>V. cholerae<\/jats:italic>\n              may experience bottlenecks, or large reductions in bacterial population sizes and genetic diversity, as it passes through the gut. Passage through the gut may select for distinct\n              <jats:italic>V. cholerae<\/jats:italic>\n              mutants that are adapted for survival and gut colonization. We did not find strong evidence for such adaptive mutations, and instead observed that passage through the gut results in modest reductions in\n              <jats:italic>V. cholerae<\/jats:italic>\n              genetic diversity, and only in some patients. These results fill a gap in our understanding of the\n              <jats:italic>V. cholerae<\/jats:italic>\n              life cycle, transmission, and evolution.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/spectrum.00785-24","type":"journal-article","created":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T13:01:32Z","timestamp":1719320492000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["<i>Vibrio cholerae<\/i>\n            O1 experiences mild bottlenecks through the gastrointestinal tract in some but not all cholera patients"],"prefix":"10.1128","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5550-712X","authenticated-orcid":true,"given":"Patrick","family":"Lypaczewski","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denise","family":"Chac","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Washington, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0000-4282","authenticated-orcid":true,"given":"Chelsea N.","family":"Dunmire","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Washington, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristine M.","family":"Tandoc","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Washington, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7082-0720","authenticated-orcid":true,"given":"Fahima","family":"Chowdhury","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division, International Center for Diarrheal Disease Research, Bangladesh, Dhaka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashraful I.","family":"Khan","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division, International Center for Diarrheal Disease Research, Bangladesh, Dhaka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3755-4763","authenticated-orcid":true,"given":"Taufiqur R.","family":"Bhuiyan","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division, International Center for Diarrheal Disease Research, Bangladesh, Dhaka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason B.","family":"Harris","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Massachusetts General Hospital, Boston, Massachusetts, USA"},{"name":"Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA"},{"name":"Division of Global Health, Massachusetts General Hospital for Children, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Regina C.","family":"LaRocque","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA"},{"name":"Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen B.","family":"Calderwood","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA"},{"name":"Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-0169-394X","authenticated-orcid":true,"given":"Edward T.","family":"Ryan","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA"},{"name":"Harvard Medical School, Boston, Massachusetts, USA"},{"name":"Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Firdausi","family":"Qadri","sequence":"additional","affiliation":[{"name":"Infectious Diseases Division, International Center for Diarrheal Disease Research, Bangladesh, Dhaka"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6819-8699","authenticated-orcid":true,"given":"B. 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