{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:31:50Z","timestamp":1785429110909,"version":"3.56.0"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T00:00:00Z","timestamp":1756771200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T00:00:00Z","timestamp":1756771200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100004704","name":"National Research Council of Thailand","doi-asserted-by":"publisher","award":["N42A660897"],"award-info":[{"award-number":["N42A660897"]}],"id":[{"id":"10.13039\/501100004704","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,9,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      The emergence of multidrug-resistant (MDR) plasmids poses a significant global public health threat. This study characterized a novel multi-replicon IncN-R MDR plasmid pCUVET19-1426.1, harboring\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>CTX-M-55<\/jats:sub>\n                      and 12 additional antimicrobial resistance genes (ARGs) in a newly identified\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      sequence type 13037, isolated from a canine prostatic abscess. Using antimicrobial resistance (AMR) profiling and genomic analysis, we elucidated the characteristics, evolution, and transferability of plasmid pCUVET19-1426.1, which contributed to the extensively drug-resistant phenotype of the bacterial strain. The plasmid is genetically related to IncN plasmids found in Enterobacterales from humans and animals in Thailand, suggesting plasmid circulation and evolution. Multiple IS\n                      <jats:italic toggle=\"yes\">26<\/jats:italic>\n                      elements enhanced the plasmid plasticity for co-integration and acquisition of ARGs, underscoring its significant clinical and public health impact. Our findings emphasize the importance of genomic insights in understanding plasmid evolution and its role in AMR development in\n                      <jats:italic toggle=\"yes\">E. coli,<\/jats:italic>\n                      causing opportunistic infections.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        This study highlights the significance of plasmid evolution to generate a new multi-replicon IncN-R plasmid encoding an extended-spectrum \u03b2-lactamase and multidrug resistance (MDR) in a new\n                        <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                        sequence type 13037 from a canine prostatic abscess. We provide insights into the genetic mechanisms facilitating the spread and persistence of resistance genes, driving the emergence of an extensively drug-resistant phenotype. The presence of an MDR plasmid in the clinical\n                        <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                        isolate critically limited antimicrobial options for extraintestinal infections in small animal veterinary medicine. The plasmid\u2019s genetic relatedness underscores the interconnectedness of human and animal health, emphasizing the importance of a One Health approach. These findings emphasize the need to enhance genomic surveillance to monitor the evolution of bacterial plasmids and their role in antimicrobial resistance development in both human and animal health.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.00790-25","type":"journal-article","created":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T13:52:17Z","timestamp":1753883537000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["IncN-R plasmid co-integration contributing to extensive drug resistance in\n                    <i>Escherichia coli<\/i>\n                    isolated from a canine prostatic abscess"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1989-0379","authenticated-orcid":false,"given":"Parinya","family":"Sroithongkham","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03cq4gr50","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Pathobiology, Faculty of Veterinary Medicine, Khon Kaen University","place":["Khon Kaen, Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7835-2191","authenticated-orcid":false,"given":"Chavin","family":"Leelapsawas","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/028wp3y58","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University","place":["Bangkok, Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rusmin","family":"Indra","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/028wp3y58","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University","place":["Bangkok, Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2668-110X","authenticated-orcid":false,"given":"Sunchai","family":"Payungporn","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/028wp3y58","id-type":"ROR","asserted-by":"publisher"}],"name":"Center of Excellence in Systems Microbiology, Department of Biochemistry, Faculty of Medicine, Chulalongkorn University","place":["Bangkok, Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2267-0104","authenticated-orcid":true,"given":"Komkiew","family":"Pinpimai","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/028wp3y58","id-type":"ROR","asserted-by":"publisher"}],"name":"Aquatic Resources Research Institute, Chulalongkorn University","place":["Bangkok, Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suppawiwat","family":"Ponglowhapan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/028wp3y58","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University","place":["Bangkok, Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8979-2440","authenticated-orcid":true,"given":"Pattrarat","family":"Chanchaithong","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/028wp3y58","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University","place":["Bangkok, Thailand"]},{"id":[{"id":"https:\/\/ror.org\/028wp3y58","id-type":"ROR","asserted-by":"publisher"}],"name":"Research Unit in Microbial Food Safety and Antimicrobial Resistance, Chulalongkorn University","place":["Bangkok, Thailand"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines11112937"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2020.00395"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkx146"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01089-23"},{"key":"e_1_3_4_6_2","unstructured":"The European Committee on Antimicrobial Susceptibility Testing. 2017. The EUCAST guideline on detection of resistance mechanisms. Available from: https:\/\/www.eucast.org\/resistance_mechanisms"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1005595"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw569"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00008-15"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1107"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-91456-0"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkaa345"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02412-14"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkaa390"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.02365"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks387"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkr225"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv421"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dks158"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13100-021-00239-x"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00038-16"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00762-15"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2019.06.007"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201489455"},{"key":"e_1_3_4_25_2","volume-title":"Acute bacterial prostatitis","author":"Davis NG","year":"2024","unstructured":"Davis NG, Silberman M. 2024. Acute bacterial prostatitis. StatPearls Publishing, Treasure Island (FL)."}],"container-title":["Microbiology Spectrum"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00790-25","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/spectrum.00790-25","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T13:05:02Z","timestamp":1756818302000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/spectrum.00790-25"}},"subtitle":[],"editor":[{"given":"Gabriele","family":"Arcari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,9,2]]},"references-count":24,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,9,2]]}},"alternative-id":["10.1128\/spectrum.00790-25"],"URL":"https:\/\/doi.org\/10.1128\/spectrum.00790-25","relation":{},"ISSN":["2165-0497"],"issn-type":[{"value":"2165-0497","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,2]]},"assertion":[{"value":"2025-03-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00790-25"}}