{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T05:33:13Z","timestamp":1785821593904,"version":"3.56.0"},"reference-count":62,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T00:00:00Z","timestamp":1762214400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Microbiol Spectr"],"published-print":{"date-parts":[[2025,11,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Candida albicans<\/jats:italic>\n                      is the primary pathogen of invasive candidiasis in most regions worldwide, but the therapy options for\n                      <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                      infections are limited, and drug tolerance further exacerbates the treatment challenges. Lysine lactylation (Kla), a recently identified post-translational modification (PTM), is observed in numerous organisms; however, the role of Kla in\n                      <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                      remains unknown. Hence, we report the first proteomic analysis of this specific modification in\n                      <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                      and discuss its potential roles in drug tolerance of\n                      <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                      . Altogether, 7,233 lactylation sites on 1,608 lactylated proteins were identified in\n                      <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                      , with the highest degree of lactylation among the species studied so far. The further bioinformatics analysis revealed that the lactylated proteins were implicated in a variety of cellular functions with diverse subcellular localizations. Additionally, we found a unique survival mode of tolerant cells in the presence of fluconazole, which will be subject to a more thorough investigation in our future studies. This paper is the first report on the lactylome of\n                      <jats:italic toggle=\"yes\">Candida<\/jats:italic>\n                      spp. and provides a reliable foundation for further research on Kla in\n                      <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                      and other human pathogens.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      This is the first report on the lactylome of\n                      <jats:italic toggle=\"yes\">Candida<\/jats:italic>\n                      spp., and it provides some valuable insights for further research on lactylation in\n                      <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                      and other human pathogens. Moreover, the observations in tolerant cells have prompted plausible hypotheses regarding the potential role of lactylation in mediating\n                      <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                      tolerance to fluconazole, thereby offering a conceptual framework for subsequent investigations. Notably, fungal tolerance to azoles, a concept distinct from resistance, represents a critical phenomenon in\n                      <jats:italic toggle=\"yes\">C. albicans<\/jats:italic>\n                      with profound clinical implications, as it directly correlates with therapeutic failure and persistent infections.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/spectrum.00810-25","type":"journal-article","created":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T12:06:51Z","timestamp":1759234011000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Comprehensive profiling of lysine lactylation in\n                    <i>Candida albicans<\/i>\n                    and exploratory analysis of fluconazole tolerance associations"],"prefix":"10.1128","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5700-7145","authenticated-orcid":false,"given":"Yuying","family":"Huang","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02drdmm93","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medical Mycology, Hospital for Skin Diseases, Institute of Dermatology, Chinese Academy of Medical Science & Peking Union Medical College","place":["Nanjing, China"]},{"id":[{"id":"https:\/\/ror.org\/01qh26a66","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Dermatology, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nana","family":"Song","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02drdmm93","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medical Mycology, Hospital for Skin Diseases, Institute 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